The morphological characteristics and modification of the oviducal glands adapted for long-term sperm storage and fertilization in Amphioctopus fangsiao
Sperm storage is an important reproductive strategy in Amphioctopus fangsiao. Sperm can be stored in the oviducal glands up to eight months before fertilization. However, studies on the underlying storage mechanisms remain limited. In this study, the morphological structures of the oviductal glands...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S235251342300011X |
| _version_ | 1797862143373082624 |
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| author | Yan Li Yu Jiang Xiaojie Sun Xiaokai Bao Xiaohui Xu Weijun Wang Zan Li Xiumei Liu Guohua Sun Bin Li Yanwei Feng Jianmin Yang |
| author_facet | Yan Li Yu Jiang Xiaojie Sun Xiaokai Bao Xiaohui Xu Weijun Wang Zan Li Xiumei Liu Guohua Sun Bin Li Yanwei Feng Jianmin Yang |
| author_sort | Yan Li |
| collection | DOAJ |
| description | Sperm storage is an important reproductive strategy in Amphioctopus fangsiao. Sperm can be stored in the oviducal glands up to eight months before fertilization. However, studies on the underlying storage mechanisms remain limited. In this study, the morphological structures of the oviductal glands and the changes adapted for sperm storage and fertilization were investigated by Hematoxylin eosin (HE) staining, transmission electron microscopy (TEM), and Alcian blue periodic acid Schiff (AB-PAS) staining. Results revealed that the nucleo-cytoplasmic ratio of the peripheral gland cells decreased and the eosinophilic secretory granules (glycoproteins) appeared in the cytoplasm and lumen during sperm storage. The cytoplasm of the central gland appeared vacuolated and AB pH 2.5 (+) secretions (acid mucins) were dispersed in the cells. These secretions may pass through the central cavity into the oviducts and be involved in zygote packaging. The spermathecae are composed of columnar epithelial ciliated cells with secretory functions. PAS (+) glycoproteins were found in the lumen, providing a microenvironment conducive to sperm survival. Cilia in the oviducts, central cavity, and spermathecae may help with sperm delivery, storage, and release, and egg transport. This study enhances our understanding of the biological reproduction process in A. fangsiao. |
| first_indexed | 2024-04-09T22:15:36Z |
| format | Article |
| id | doaj.art-e0e1c6ccde8a40749d74101c61c63ff4 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-04-09T22:15:36Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-e0e1c6ccde8a40749d74101c61c63ff42023-03-23T04:36:14ZengElsevierAquaculture Reports2352-51342023-04-0129101472The morphological characteristics and modification of the oviducal glands adapted for long-term sperm storage and fertilization in Amphioctopus fangsiaoYan Li0Yu Jiang1Xiaojie Sun2Xiaokai Bao3Xiaohui Xu4Weijun Wang5Zan Li6Xiumei Liu7Guohua Sun8Bin Li9Yanwei Feng10Jianmin Yang11School of Agriculture, Ludong University, Yantai 264025, ChinaSchool of Agriculture, Ludong University, Yantai 264025, ChinaSchool of Agriculture, Ludong University, Yantai 264025, ChinaSchool of Agriculture, Ludong University, Yantai 264025, ChinaSchool of Agriculture, Ludong University, Yantai 264025, ChinaSchool of Agriculture, Ludong University, Yantai 264025, China; Jiangsu Baoyuan Biotechnology Co. Ltd., Lianyungang 222100, ChinaSchool of Agriculture, Ludong University, Yantai 264025, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaSchool of Agriculture, Ludong University, Yantai 264025, ChinaYantai Haiyu Marine Science and Technology Co. Ltd., Yantai 264004, ChinaSchool of Agriculture, Ludong University, Yantai 264025, China; Corresponding authors.School of Agriculture, Ludong University, Yantai 264025, China; Corresponding authors.Sperm storage is an important reproductive strategy in Amphioctopus fangsiao. Sperm can be stored in the oviducal glands up to eight months before fertilization. However, studies on the underlying storage mechanisms remain limited. In this study, the morphological structures of the oviductal glands and the changes adapted for sperm storage and fertilization were investigated by Hematoxylin eosin (HE) staining, transmission electron microscopy (TEM), and Alcian blue periodic acid Schiff (AB-PAS) staining. Results revealed that the nucleo-cytoplasmic ratio of the peripheral gland cells decreased and the eosinophilic secretory granules (glycoproteins) appeared in the cytoplasm and lumen during sperm storage. The cytoplasm of the central gland appeared vacuolated and AB pH 2.5 (+) secretions (acid mucins) were dispersed in the cells. These secretions may pass through the central cavity into the oviducts and be involved in zygote packaging. The spermathecae are composed of columnar epithelial ciliated cells with secretory functions. PAS (+) glycoproteins were found in the lumen, providing a microenvironment conducive to sperm survival. Cilia in the oviducts, central cavity, and spermathecae may help with sperm delivery, storage, and release, and egg transport. This study enhances our understanding of the biological reproduction process in A. fangsiao.http://www.sciencedirect.com/science/article/pii/S235251342300011XAmphiOctopus fangsiaoOviducal glandsSperm storageFertilizationMorphological modification |
| spellingShingle | Yan Li Yu Jiang Xiaojie Sun Xiaokai Bao Xiaohui Xu Weijun Wang Zan Li Xiumei Liu Guohua Sun Bin Li Yanwei Feng Jianmin Yang The morphological characteristics and modification of the oviducal glands adapted for long-term sperm storage and fertilization in Amphioctopus fangsiao Aquaculture Reports AmphiOctopus fangsiao Oviducal glands Sperm storage Fertilization Morphological modification |
| title | The morphological characteristics and modification of the oviducal glands adapted for long-term sperm storage and fertilization in Amphioctopus fangsiao |
| title_full | The morphological characteristics and modification of the oviducal glands adapted for long-term sperm storage and fertilization in Amphioctopus fangsiao |
| title_fullStr | The morphological characteristics and modification of the oviducal glands adapted for long-term sperm storage and fertilization in Amphioctopus fangsiao |
| title_full_unstemmed | The morphological characteristics and modification of the oviducal glands adapted for long-term sperm storage and fertilization in Amphioctopus fangsiao |
| title_short | The morphological characteristics and modification of the oviducal glands adapted for long-term sperm storage and fertilization in Amphioctopus fangsiao |
| title_sort | morphological characteristics and modification of the oviducal glands adapted for long term sperm storage and fertilization in amphioctopus fangsiao |
| topic | AmphiOctopus fangsiao Oviducal glands Sperm storage Fertilization Morphological modification |
| url | http://www.sciencedirect.com/science/article/pii/S235251342300011X |
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