Correlation of the ALA-PDT Treatment Efficacy and the HPV Genotype Profile of Genital Warts after Cryotherapy Failure and Podophyllotoxin Therapy in Male Patients
Background: Genital warts are the manifestation of the human papillomavirus (HPV) infection, which may last for weeks or months before the clinical presentation. The primary aim of the study was the correlation of the DNA HPV genotypes eradication with the treatment response in male patients with pe...
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| Format: | Article |
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MDPI AG
2021-02-01
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| Online Access: | https://www.mdpi.com/2075-1729/11/2/146 |
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| author | Witold Owczarek Monika Slowinska Irena Walecka Magdalena Ciazynska Dorota Nowicka Leszek Walczak Elwira Paluchowska |
| author_facet | Witold Owczarek Monika Slowinska Irena Walecka Magdalena Ciazynska Dorota Nowicka Leszek Walczak Elwira Paluchowska |
| author_sort | Witold Owczarek |
| collection | DOAJ |
| description | Background: Genital warts are the manifestation of the human papillomavirus (HPV) infection, which may last for weeks or months before the clinical presentation. The primary aim of the study was the correlation of the DNA HPV genotypes eradication with the treatment response in male patients with persistent genital warts. Methods: Twenty-one male patients (age range: 22–58) after failure of cryotherapy and podophyllotoxin treatment were enrolled in the study. Genetic tests (Real Time - PCR method) analyzed the presence of DNA-HPV before and 6 months after four sessions (4 weeks apart) of photodynamic therapy with 5-aminolaevulinic acid (ALA-PDT). The treatment efficacy was evaluated before each PDT session and at the end of the study. Results: The single HPV DNA type was present in 15/21 of the patients (13/15 HPV6). The high-risk HPV types were found in 8/21 subjects, of which 6/8 had several types. Six months after four sessions of PDT, complete response was found in 16/21 (76.19%; <i>p</i> = 0.0007) of patients, and DNA HPV clearance was found in 66.67% (<i>p</i> = 0.03). The eradication rate differed among patients with primary low-risk and high-risk HPV types—76.92% (10/13; <i>p</i> = 0.0003) and 50% (4/8; <i>p</i> = 0.05) respectively. Conclusion: ALA-PDT is an effective treatment even after the failure of previous modalities. The persistence of clinical lesions and high oncological risk HPV types should be an indication for treatment prolongation. |
| first_indexed | 2024-03-09T00:53:59Z |
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| id | doaj.art-e0e4aa6459bb4364ba7a3ecdc8d119cf |
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| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T00:53:59Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-e0e4aa6459bb4364ba7a3ecdc8d119cf2023-12-11T17:03:41ZengMDPI AGLife2075-17292021-02-0111214610.3390/life11020146Correlation of the ALA-PDT Treatment Efficacy and the HPV Genotype Profile of Genital Warts after Cryotherapy Failure and Podophyllotoxin Therapy in Male PatientsWitold Owczarek0Monika Slowinska1Irena Walecka2Magdalena Ciazynska3Dorota Nowicka4Leszek Walczak5Elwira Paluchowska6Department of Dermatology, Military Institute of Medicine, 01-755 Warsaw, PolandDepartment of Dermatology, Military Institute of Medicine, 01-755 Warsaw, PolandClinic of Dermatology, Centre of Postgraduate Medical Education, Central Clinical Hospital of the Ministry of the Interior, 02-097 Warsaw, PolandDepartment of Proliferative Diseases, Nicolaus Copernicus Multidisciplinary Centre for Oncology and Traumatology, 87-100 Torun, PolandDepartment of Dermatology and Venereology, Medical University of Warsaw, 02-091 Warsaw, PolandDepartment of Dermatology, Military Institute of Medicine, 01-755 Warsaw, PolandDepartment of Dermatology, Military Institute of Medicine, 01-755 Warsaw, PolandBackground: Genital warts are the manifestation of the human papillomavirus (HPV) infection, which may last for weeks or months before the clinical presentation. The primary aim of the study was the correlation of the DNA HPV genotypes eradication with the treatment response in male patients with persistent genital warts. Methods: Twenty-one male patients (age range: 22–58) after failure of cryotherapy and podophyllotoxin treatment were enrolled in the study. Genetic tests (Real Time - PCR method) analyzed the presence of DNA-HPV before and 6 months after four sessions (4 weeks apart) of photodynamic therapy with 5-aminolaevulinic acid (ALA-PDT). The treatment efficacy was evaluated before each PDT session and at the end of the study. Results: The single HPV DNA type was present in 15/21 of the patients (13/15 HPV6). The high-risk HPV types were found in 8/21 subjects, of which 6/8 had several types. Six months after four sessions of PDT, complete response was found in 16/21 (76.19%; <i>p</i> = 0.0007) of patients, and DNA HPV clearance was found in 66.67% (<i>p</i> = 0.03). The eradication rate differed among patients with primary low-risk and high-risk HPV types—76.92% (10/13; <i>p</i> = 0.0003) and 50% (4/8; <i>p</i> = 0.05) respectively. Conclusion: ALA-PDT is an effective treatment even after the failure of previous modalities. The persistence of clinical lesions and high oncological risk HPV types should be an indication for treatment prolongation.https://www.mdpi.com/2075-1729/11/2/146genital wartsALA-PDTDNA HPVreal-time PCRtreatment efficacy |
| spellingShingle | Witold Owczarek Monika Slowinska Irena Walecka Magdalena Ciazynska Dorota Nowicka Leszek Walczak Elwira Paluchowska Correlation of the ALA-PDT Treatment Efficacy and the HPV Genotype Profile of Genital Warts after Cryotherapy Failure and Podophyllotoxin Therapy in Male Patients Life genital warts ALA-PDT DNA HPV real-time PCR treatment efficacy |
| title | Correlation of the ALA-PDT Treatment Efficacy and the HPV Genotype Profile of Genital Warts after Cryotherapy Failure and Podophyllotoxin Therapy in Male Patients |
| title_full | Correlation of the ALA-PDT Treatment Efficacy and the HPV Genotype Profile of Genital Warts after Cryotherapy Failure and Podophyllotoxin Therapy in Male Patients |
| title_fullStr | Correlation of the ALA-PDT Treatment Efficacy and the HPV Genotype Profile of Genital Warts after Cryotherapy Failure and Podophyllotoxin Therapy in Male Patients |
| title_full_unstemmed | Correlation of the ALA-PDT Treatment Efficacy and the HPV Genotype Profile of Genital Warts after Cryotherapy Failure and Podophyllotoxin Therapy in Male Patients |
| title_short | Correlation of the ALA-PDT Treatment Efficacy and the HPV Genotype Profile of Genital Warts after Cryotherapy Failure and Podophyllotoxin Therapy in Male Patients |
| title_sort | correlation of the ala pdt treatment efficacy and the hpv genotype profile of genital warts after cryotherapy failure and podophyllotoxin therapy in male patients |
| topic | genital warts ALA-PDT DNA HPV real-time PCR treatment efficacy |
| url | https://www.mdpi.com/2075-1729/11/2/146 |
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