Co-isolation of extracellular vesicles and high-density lipoproteins using density gradient ultracentrifugation
Extracellular vesicles (EVs) facilitate intercellular communication by carrying bioactive molecules such as proteins, messenger RNA, and micro (mi)RNAs. Recently, high-density lipoproteins (HDL) isolated from human plasma were also reported to transport miRNA to other cells. HDL, when isolated from...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-07-01
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| Series: | Journal of Extracellular Vesicles |
| Subjects: | |
| Online Access: | http://www.journalofextracellularvesicles.net/index.php/jev/article/download/23262/34509 |
| _version_ | 1819109464992645120 |
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| author | Yuana Yuana Johannes Levels Anita Grootemaat Auguste Sturk Rienk Nieuwland |
| author_facet | Yuana Yuana Johannes Levels Anita Grootemaat Auguste Sturk Rienk Nieuwland |
| author_sort | Yuana Yuana |
| collection | DOAJ |
| description | Extracellular vesicles (EVs) facilitate intercellular communication by carrying bioactive molecules such as proteins, messenger RNA, and micro (mi)RNAs. Recently, high-density lipoproteins (HDL) isolated from human plasma were also reported to transport miRNA to other cells. HDL, when isolated from human plasma, ranges in density between 1.063 and 1.21 g/mL, which grossly overlap with the reported density of EVs. Consequently, HDL and EV will be co-isolated when using density gradient ultracentrifugation. Thus, more stringent isolation/separation procedures of EV and HDL are essential to know their relative contribution to the pool of circulating bioactive molecules. |
| first_indexed | 2024-12-22T03:26:15Z |
| format | Article |
| id | doaj.art-e0e6636398ba4afc89434d0c26a76554 |
| institution | Directory Open Access Journal |
| issn | 2001-3078 |
| language | English |
| last_indexed | 2024-12-22T03:26:15Z |
| publishDate | 2014-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Extracellular Vesicles |
| spelling | doaj.art-e0e6636398ba4afc89434d0c26a765542022-12-21T18:40:36ZengWileyJournal of Extracellular Vesicles2001-30782014-07-01301510.3402/jev.v3.2326223262Co-isolation of extracellular vesicles and high-density lipoproteins using density gradient ultracentrifugationYuana Yuana0Johannes Levels1Anita Grootemaat2Auguste Sturk3Rienk Nieuwland4 Department of Clinical Chemistry, Academic Medical Centre of the University of Amsterdam, Amsterdam, The Netherlands Department of Experimental Vascular Medicine, Academic Medical Centre of the University of Amsterdam, Amsterdam, The Netherlands Department of Clinical Chemistry, Academic Medical Centre of the University of Amsterdam, Amsterdam, The Netherlands Department of Clinical Chemistry, Academic Medical Centre of the University of Amsterdam, Amsterdam, The Netherlands Department of Clinical Chemistry, Academic Medical Centre of the University of Amsterdam, Amsterdam, The NetherlandsExtracellular vesicles (EVs) facilitate intercellular communication by carrying bioactive molecules such as proteins, messenger RNA, and micro (mi)RNAs. Recently, high-density lipoproteins (HDL) isolated from human plasma were also reported to transport miRNA to other cells. HDL, when isolated from human plasma, ranges in density between 1.063 and 1.21 g/mL, which grossly overlap with the reported density of EVs. Consequently, HDL and EV will be co-isolated when using density gradient ultracentrifugation. Thus, more stringent isolation/separation procedures of EV and HDL are essential to know their relative contribution to the pool of circulating bioactive molecules.http://www.journalofextracellularvesicles.net/index.php/jev/article/download/23262/34509IsolationRNAlipoproteinsextracellular vesicles |
| spellingShingle | Yuana Yuana Johannes Levels Anita Grootemaat Auguste Sturk Rienk Nieuwland Co-isolation of extracellular vesicles and high-density lipoproteins using density gradient ultracentrifugation Journal of Extracellular Vesicles Isolation RNA lipoproteins extracellular vesicles |
| title | Co-isolation of extracellular vesicles and high-density lipoproteins using density gradient ultracentrifugation |
| title_full | Co-isolation of extracellular vesicles and high-density lipoproteins using density gradient ultracentrifugation |
| title_fullStr | Co-isolation of extracellular vesicles and high-density lipoproteins using density gradient ultracentrifugation |
| title_full_unstemmed | Co-isolation of extracellular vesicles and high-density lipoproteins using density gradient ultracentrifugation |
| title_short | Co-isolation of extracellular vesicles and high-density lipoproteins using density gradient ultracentrifugation |
| title_sort | co isolation of extracellular vesicles and high density lipoproteins using density gradient ultracentrifugation |
| topic | Isolation RNA lipoproteins extracellular vesicles |
| url | http://www.journalofextracellularvesicles.net/index.php/jev/article/download/23262/34509 |
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