Simultaneous saccharification and fermentation of cassava waste for ethanol production

The efficiency of enzymatic and microbial saccharification of cassava waste for ethanol production was investigated and the effective parameters were optimized. The mixture of amylase and amyloglucosidase (AMG) resulted in a significantly higher rate of saccharification (79.6%) than the amylase alon...

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Main Authors: C. Pothiraj, A. Arun, M. Eyini
Format: Article
Language:English
Published: Alpha Creation Enterprise 2015-03-01
Series:Biofuel Research Journal
Subjects:
Online Access:http://www.biofueljournal.com/pdf_8441_3ffbe8fe808712c0641b1b240122d4d1.html
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author C. Pothiraj
A. Arun
M. Eyini
author_facet C. Pothiraj
A. Arun
M. Eyini
author_sort C. Pothiraj
collection DOAJ
description The efficiency of enzymatic and microbial saccharification of cassava waste for ethanol production was investigated and the effective parameters were optimized. The mixture of amylase and amyloglucosidase (AMG) resulted in a significantly higher rate of saccharification (79.6%) than the amylase alone (68.7%). Simultaneous saccharification and fermentation (SSF) yielded 6.2 g L-1 ethanol representing 64.5% of the theoretical yield. Saccharification and fermentation using pure and co-cultures of fungal isolates including Rhizopus stolonifer, Aspergillus terreus, Saccharomyces diastaticus and Zymomonas mobilis revealed that the co-culture system involving S. diastaticus and Z. mobilis was highly suitable for the bio-conversion of cassava waste into ethanol, resulting in 20.4 g L-1 in 36 h (91.3% of the theoretical yield).
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spelling doaj.art-e0e6e784f97e4ba7a36d89878b5632512024-03-26T15:13:22ZengAlpha Creation EnterpriseBiofuel Research Journal2292-87822292-87822015-03-01211962028441Simultaneous saccharification and fermentation of cassava waste for ethanol productionC. Pothiraj0A. Arun1M. Eyini2Department of Botany, Alagappa Government Arts College, Karaikudi 630003, Tamilnadu, India.Department of Energy Science, Alagappa University, Karaikudi 630004, Tamilnadu, India.Research Centre in Botany, Thiagarajar College, Madurai 625009, Tamilnadu, India.The efficiency of enzymatic and microbial saccharification of cassava waste for ethanol production was investigated and the effective parameters were optimized. The mixture of amylase and amyloglucosidase (AMG) resulted in a significantly higher rate of saccharification (79.6%) than the amylase alone (68.7%). Simultaneous saccharification and fermentation (SSF) yielded 6.2 g L-1 ethanol representing 64.5% of the theoretical yield. Saccharification and fermentation using pure and co-cultures of fungal isolates including Rhizopus stolonifer, Aspergillus terreus, Saccharomyces diastaticus and Zymomonas mobilis revealed that the co-culture system involving S. diastaticus and Z. mobilis was highly suitable for the bio-conversion of cassava waste into ethanol, resulting in 20.4 g L-1 in 36 h (91.3% of the theoretical yield).http://www.biofueljournal.com/pdf_8441_3ffbe8fe808712c0641b1b240122d4d1.htmlCassava wasteEthanolEnzymatic and microbial saccharificationSimultaneous saccharification and fermentation (SSF)
spellingShingle C. Pothiraj
A. Arun
M. Eyini
Simultaneous saccharification and fermentation of cassava waste for ethanol production
Biofuel Research Journal
Cassava waste
Ethanol
Enzymatic and microbial saccharification
Simultaneous saccharification and fermentation (SSF)
title Simultaneous saccharification and fermentation of cassava waste for ethanol production
title_full Simultaneous saccharification and fermentation of cassava waste for ethanol production
title_fullStr Simultaneous saccharification and fermentation of cassava waste for ethanol production
title_full_unstemmed Simultaneous saccharification and fermentation of cassava waste for ethanol production
title_short Simultaneous saccharification and fermentation of cassava waste for ethanol production
title_sort simultaneous saccharification and fermentation of cassava waste for ethanol production
topic Cassava waste
Ethanol
Enzymatic and microbial saccharification
Simultaneous saccharification and fermentation (SSF)
url http://www.biofueljournal.com/pdf_8441_3ffbe8fe808712c0641b1b240122d4d1.html
work_keys_str_mv AT cpothiraj simultaneoussaccharificationandfermentationofcassavawasteforethanolproduction
AT aarun simultaneoussaccharificationandfermentationofcassavawasteforethanolproduction
AT meyini simultaneoussaccharificationandfermentationofcassavawasteforethanolproduction