Effect of Carvacrol on O157 and non-O157 Shiga Toxin Producing Escherichia coli
Shiga toxin Escherichia coli (STEC) strains are important foodborne bacteria linked to diarrhea, enteritis, hemolytic-uremic syndrome and in some cases death. E. coli O157:H7 is the most common strain amongst STECs however non-O157 STECs have been connected with several outbreaks on an international...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-09-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/1061 |
| _version_ | 1797215458152153088 |
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| author | Alexandros Stratakos Filip Sima Mark Linton Carmel Kelly Laurette Pinkerton Nicolae Corcionivoschi |
| author_facet | Alexandros Stratakos Filip Sima Mark Linton Carmel Kelly Laurette Pinkerton Nicolae Corcionivoschi |
| author_sort | Alexandros Stratakos |
| collection | DOAJ |
| description | Shiga toxin Escherichia coli (STEC) strains are important foodborne bacteria linked to diarrhea, enteritis, hemolytic-uremic syndrome and in some cases death. E. coli O157:H7 is the most common strain amongst STECs however non-O157 STECs have been connected with several outbreaks on an international level. The use of natural plant extracts to reduce the risk from foodborne pathogens is gaining increasing importance. Therefore in this study, we tested the antibacterial effect of carvacrol, a major component of oregano essential oil, on E. coli serogroups O157, O26, O45, O103, O111, O121, O145 as well as serogroup O104 responsible for the massive outbreak in Germany in 2011. Carvacrol showed antibacterial effect on all strains tested. The relative electric conductivity was assessed in order to investigate the changes in membrane permeability and thus to investigate the antimicrobial mechanism of carvacrol. Results showed that the relative conductivity increased with increasing concentrations of carvacrol which showed that there was an increasing leakage of electrolytes due to disruption of the cell membrane. The data presented here revealed that carvacrol has the potential to be used as a natural antimicrobial against STECs. |
| first_indexed | 2024-04-24T11:30:23Z |
| format | Article |
| id | doaj.art-e0e90931ceff463aa059f12b89f2b8d2 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:30:23Z |
| publishDate | 2023-09-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-e0e90931ceff463aa059f12b89f2b8d22024-04-10T10:48:43ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-09-015011261261061Effect of Carvacrol on O157 and non-O157 Shiga Toxin Producing Escherichia coliAlexandros Stratakos0Filip Sima1Mark Linton2Carmel Kelly3Laurette Pinkerton4Nicolae Corcionivoschi5Agri-Food and Biosciences Institute, Veterinary Sciences Division, Bacteriology Branch, BT9 5PX-Belfast, Newforge Lane, Northern Ireland, United Kingdom.Agri-Food and Biosciences Institute, Veterinary Sciences Division, Bacteriology Branch, BT9 5PX-Belfast, Newforge Lane, Northern Ireland, United Kingdom.Agri-Food and Biosciences Institute, Veterinary Sciences Division, Bacteriology Branch, BT9 5PX-Belfast, Newforge Lane, Northern Ireland, United Kingdom.Agri-Food and Biosciences Institute, Veterinary Sciences Division, Bacteriology Branch, BT9 5PX-Belfast, Newforge Lane, Northern Ireland, United Kingdom.Agri-Food and Biosciences Institute, Veterinary Sciences Division, Bacteriology Branch, BT9 5PX-Belfast, Newforge Lane, Northern Ireland, United Kingdom.Agri-Food and Biosciences Institute, Veterinary Sciences Division, Bacteriology Branch, BT9 5PX-Belfast, Newforge Lane, Northern Ireland, United KingdomShiga toxin Escherichia coli (STEC) strains are important foodborne bacteria linked to diarrhea, enteritis, hemolytic-uremic syndrome and in some cases death. E. coli O157:H7 is the most common strain amongst STECs however non-O157 STECs have been connected with several outbreaks on an international level. The use of natural plant extracts to reduce the risk from foodborne pathogens is gaining increasing importance. Therefore in this study, we tested the antibacterial effect of carvacrol, a major component of oregano essential oil, on E. coli serogroups O157, O26, O45, O103, O111, O121, O145 as well as serogroup O104 responsible for the massive outbreak in Germany in 2011. Carvacrol showed antibacterial effect on all strains tested. The relative electric conductivity was assessed in order to investigate the changes in membrane permeability and thus to investigate the antimicrobial mechanism of carvacrol. Results showed that the relative conductivity increased with increasing concentrations of carvacrol which showed that there was an increasing leakage of electrolytes due to disruption of the cell membrane. The data presented here revealed that carvacrol has the potential to be used as a natural antimicrobial against STECs.https://spasb.ro/index.php/public_html/article/view/1061antibacterialcarvacrolnon-o157 e. colio 157 e. colipermeability |
| spellingShingle | Alexandros Stratakos Filip Sima Mark Linton Carmel Kelly Laurette Pinkerton Nicolae Corcionivoschi Effect of Carvacrol on O157 and non-O157 Shiga Toxin Producing Escherichia coli Scientific Papers Animal Science and Biotechnologies antibacterial carvacrol non-o157 e. coli o 157 e. coli permeability |
| title | Effect of Carvacrol on O157 and non-O157 Shiga Toxin Producing Escherichia coli |
| title_full | Effect of Carvacrol on O157 and non-O157 Shiga Toxin Producing Escherichia coli |
| title_fullStr | Effect of Carvacrol on O157 and non-O157 Shiga Toxin Producing Escherichia coli |
| title_full_unstemmed | Effect of Carvacrol on O157 and non-O157 Shiga Toxin Producing Escherichia coli |
| title_short | Effect of Carvacrol on O157 and non-O157 Shiga Toxin Producing Escherichia coli |
| title_sort | effect of carvacrol on o157 and non o157 shiga toxin producing escherichia coli |
| topic | antibacterial carvacrol non-o157 e. coli o 157 e. coli permeability |
| url | https://spasb.ro/index.php/public_html/article/view/1061 |
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