Clonal dissemination of successful emerging clone mecA-MRSA t304/ST6 among humans and hedgehogs in the Helsinki metropolitan area in Finland
Methicillin-resistant Staphylococcus aureus (MRSA) carrying mecC gene (mecC-MRSA) is frequently reported among European hedgehogs (Europeaus erineaus) due to co-evolutionary adaptation to dermatophyte infection in European hedgehogs. The occurrence of MRSA in European hedgehogs in Finland is unknown...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | One Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352771423000368 |
| _version_ | 1797805858414919680 |
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| author | Johansson Venla Al-Mustapha Ahmad Heljanko Viivi Lindholm Laura Salmenlinna Saara Sainmaa Sanna Heikinheimo Annamari |
| author_facet | Johansson Venla Al-Mustapha Ahmad Heljanko Viivi Lindholm Laura Salmenlinna Saara Sainmaa Sanna Heikinheimo Annamari |
| author_sort | Johansson Venla |
| collection | DOAJ |
| description | Methicillin-resistant Staphylococcus aureus (MRSA) carrying mecC gene (mecC-MRSA) is frequently reported among European hedgehogs (Europeaus erineaus) due to co-evolutionary adaptation to dermatophyte infection in European hedgehogs. The occurrence of MRSA in European hedgehogs in Finland is unknown. Consequently, we investigated the occurrence of MRSA in wild hedgehogs from urban Helsinki metropolitan area in 2020–2021 and applied whole genome sequencing (WGS) to further characterize the studied isolates and compared them with human clinical MRSA isolates. Altogether 115 dead hedgehogs were screened for MRSA using selective cultivation methods. Presumptive MRSA isolates were tested for antimicrobial susceptibility and confirmed MRSA isolates were further characterized by spa-typing and WGS. Hedgehog derived MRSA isolates were compared with clinical human MRSA isolates using core genome multilocus sequence typing (cgMLST). In total MRSA was recovered from 11 out of 115 (10%) hedgehogs. Among these four different spa types (t304; n = 4, t8835; n = 4, t5133; n = 2 and t622; n = 1) and three different sequence types (STs) (ST6; n = 6, ST7663; n = 4 and ST2840; n = 1) were identified. From the studied MRSA isolates seven harboured the mecA gene (mecA-MRSA) and four were identified as mecC-MRSA. All mecA-MRSA isolates carried immune evasion cluster genes, and one isolate was positive for Panton-Valentine leukocidin. cgMLST comparison revealed close genetic relatedness among three hedgehog and two human mecA-MRSA isolates all belonging to t304/ST6. Our results suggest a clonal dissemination of a successful MRSA clone among humans and hedgehogs. Further studies are warranted to investigate the sources and dissemination of such clone in urban environments. We observed a relatively low occurrence of mecC-MRSA in Finnish hedgehogs. |
| first_indexed | 2024-03-13T05:58:27Z |
| format | Article |
| id | doaj.art-e0e9ae7dafa14fbb9562806df82125cb |
| institution | Directory Open Access Journal |
| issn | 2352-7714 |
| language | English |
| last_indexed | 2024-03-13T05:58:27Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | One Health |
| spelling | doaj.art-e0e9ae7dafa14fbb9562806df82125cb2023-06-13T04:12:22ZengElsevierOne Health2352-77142023-06-0116100516Clonal dissemination of successful emerging clone mecA-MRSA t304/ST6 among humans and hedgehogs in the Helsinki metropolitan area in FinlandJohansson Venla0Al-Mustapha Ahmad1Heljanko Viivi2Lindholm Laura3Salmenlinna Saara4Sainmaa Sanna5Heikinheimo Annamari6Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Agnes Sjöberginkatu 2, PO Box 66, 00790, Helsinki, Finland; Corresponding author at: DVM, Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Agnes Sjöberginkatu 2, P.O. Box 66, Helsinki, Finland.Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Agnes Sjöberginkatu 2, PO Box 66, 00790, Helsinki, FinlandDepartment of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Agnes Sjöberginkatu 2, PO Box 66, 00790, Helsinki, FinlandExpert Microbiology Unit, Department of Health Security, Finnish Institute for Health and Welfare, Mannerheimintie 166, PO Box 30, 00271, Helsinki, FinlandExpert Microbiology Unit, Department of Health Security, Finnish Institute for Health and Welfare, Mannerheimintie 166, PO Box 30, 00271, Helsinki, FinlandKorkeasaari Zoo, Mustikkamaanpolku 12, PO Box 1000, 0081 Helsinki, FinlandDepartment of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Agnes Sjöberginkatu 2, PO Box 66, 00790, Helsinki, Finland; Microbiology Unit, Finnish Food Authority, Mustialankatu 3, PO Box 200, 00790 Helsinki, FinlandMethicillin-resistant Staphylococcus aureus (MRSA) carrying mecC gene (mecC-MRSA) is frequently reported among European hedgehogs (Europeaus erineaus) due to co-evolutionary adaptation to dermatophyte infection in European hedgehogs. The occurrence of MRSA in European hedgehogs in Finland is unknown. Consequently, we investigated the occurrence of MRSA in wild hedgehogs from urban Helsinki metropolitan area in 2020–2021 and applied whole genome sequencing (WGS) to further characterize the studied isolates and compared them with human clinical MRSA isolates. Altogether 115 dead hedgehogs were screened for MRSA using selective cultivation methods. Presumptive MRSA isolates were tested for antimicrobial susceptibility and confirmed MRSA isolates were further characterized by spa-typing and WGS. Hedgehog derived MRSA isolates were compared with clinical human MRSA isolates using core genome multilocus sequence typing (cgMLST). In total MRSA was recovered from 11 out of 115 (10%) hedgehogs. Among these four different spa types (t304; n = 4, t8835; n = 4, t5133; n = 2 and t622; n = 1) and three different sequence types (STs) (ST6; n = 6, ST7663; n = 4 and ST2840; n = 1) were identified. From the studied MRSA isolates seven harboured the mecA gene (mecA-MRSA) and four were identified as mecC-MRSA. All mecA-MRSA isolates carried immune evasion cluster genes, and one isolate was positive for Panton-Valentine leukocidin. cgMLST comparison revealed close genetic relatedness among three hedgehog and two human mecA-MRSA isolates all belonging to t304/ST6. Our results suggest a clonal dissemination of a successful MRSA clone among humans and hedgehogs. Further studies are warranted to investigate the sources and dissemination of such clone in urban environments. We observed a relatively low occurrence of mecC-MRSA in Finnish hedgehogs.http://www.sciencedirect.com/science/article/pii/S2352771423000368Methicillin-resistant Staphylococcus aureusWild animalsAntimicrobial resistanceHuman–wildlife interfacet304Hedgehog |
| spellingShingle | Johansson Venla Al-Mustapha Ahmad Heljanko Viivi Lindholm Laura Salmenlinna Saara Sainmaa Sanna Heikinheimo Annamari Clonal dissemination of successful emerging clone mecA-MRSA t304/ST6 among humans and hedgehogs in the Helsinki metropolitan area in Finland One Health Methicillin-resistant Staphylococcus aureus Wild animals Antimicrobial resistance Human–wildlife interface t304 Hedgehog |
| title | Clonal dissemination of successful emerging clone mecA-MRSA t304/ST6 among humans and hedgehogs in the Helsinki metropolitan area in Finland |
| title_full | Clonal dissemination of successful emerging clone mecA-MRSA t304/ST6 among humans and hedgehogs in the Helsinki metropolitan area in Finland |
| title_fullStr | Clonal dissemination of successful emerging clone mecA-MRSA t304/ST6 among humans and hedgehogs in the Helsinki metropolitan area in Finland |
| title_full_unstemmed | Clonal dissemination of successful emerging clone mecA-MRSA t304/ST6 among humans and hedgehogs in the Helsinki metropolitan area in Finland |
| title_short | Clonal dissemination of successful emerging clone mecA-MRSA t304/ST6 among humans and hedgehogs in the Helsinki metropolitan area in Finland |
| title_sort | clonal dissemination of successful emerging clone meca mrsa t304 st6 among humans and hedgehogs in the helsinki metropolitan area in finland |
| topic | Methicillin-resistant Staphylococcus aureus Wild animals Antimicrobial resistance Human–wildlife interface t304 Hedgehog |
| url | http://www.sciencedirect.com/science/article/pii/S2352771423000368 |
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