Circ-DDX5 inhibits the proliferation and invasion of human breast cancer cell lines by targeting miR-3940
Objective To investigate the expression of circular-RNA DDX5(circ-DDX5) in breast cancer tissues and its relationship with the clinical stage of breast cancer patients, and to analyze the regulatory mechanism of circ-DDX5 on the proliferation and invasion of human breast cancer cell line. Methods Th...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2024-01-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2024-44-1-1.pdf |
| _version_ | 1797366829689077760 |
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| author | LI Jiangli, SUN Jing, TANG Yijun, GUO Junlan, CHEN Bo, GUO Shengnan |
| author_facet | LI Jiangli, SUN Jing, TANG Yijun, GUO Junlan, CHEN Bo, GUO Shengnan |
| author_sort | LI Jiangli, SUN Jing, TANG Yijun, GUO Junlan, CHEN Bo, GUO Shengnan |
| collection | DOAJ |
| description | Objective To investigate the expression of circular-RNA DDX5(circ-DDX5) in breast cancer tissues and its relationship with the clinical stage of breast cancer patients, and to analyze the regulatory mechanism of circ-DDX5 on the proliferation and invasion of human breast cancer cell line. Methods The expression level of circ-DDX5 in breast cancer tissues and its correlation with the clinical stage of breast cancer patients were analyzed by TCGA database. Bioinformatics analysis and dual-luciferase reporter gene experiments verified the targeting relationship between circ-DDX5 and miR-3940. The correlation between circ-DDX5 and miR-3940 expression in breast cancer tissues was analyzed by TCGA database. The expression level of circ-DDX5 in breast cancer SK-BR-3, MDA-MB-231, BT-549, MCF-7, and HCC-1937 cells was detected by RT-qPCR. The circ-DDX5 over-expression plasmid and negative control plasmid were transfected into MDA-MB-231 cells, which were named circ-DDX5 group and NC group, respectively. The proliferation and invasion of MDA-MB-231 cells in the circ-DDX5 group and the NC group were detected by colony formation assay and Transwell assay. The expressions of proliferation phenotype protein and invasion phenotype protein of MDA-MB-231 cells were detected by Western blot. The expression level of miR-3940 in MDA-MB-231 cells of circ-DDX5 group and NC group was detected by RT-qPCR. Results The expression of circ-DDX5 in breast cancer tissues was lower than that in adjacent tissues (P＜0.01) and the expression level of circ-DDX5 was negatively correlated with the clinical stage of breast cancer patients (P＜0.01). There was a targeting relationship between circ-DDX5 and miR-3940 (P＜0.01). The expression of circ-DDX5 and miR-3940 in breast cancer tissue was negatively correlated (P＜0.01). The expression of circ-DDX5 in human breast cancer cell lines was lower than that in immortalized breast epithelial cells MCF-10A (P＜0.05 or P＜0.01). Compared with the NC group, the over-expression of circ-DDX5 could significantly inhibit the proliferation and invasion of MDA-MB-231 cells(P＜0.01), as well as the proliferation phenotype proteins (cyclin C, CDK3) and invasion phenotype proteins (Snail, vimentin) expression (P＜0.01) and miR-3940 expression (P＜0.01). Conclusions The expression of circ-DDX5 in breast cancer tissues and cells is low. circ-DDX5 inhibits the proliferation and invasion of breast cancer MDA-MB-231 cells by targeting the expression of miR-3940. |
| first_indexed | 2024-03-08T17:09:17Z |
| format | Article |
| id | doaj.art-e0ec50a3f5d946d9971da1fd374fbbb4 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T17:09:17Z |
| publishDate | 2024-01-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-e0ec50a3f5d946d9971da1fd374fbbb42024-01-04T01:09:47ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252024-01-014411710.16352/j.issn.1001-6325.2024.01.0001Circ-DDX5 inhibits the proliferation and invasion of human breast cancer cell lines by targeting miR-3940LI Jiangli, SUN Jing, TANG Yijun, GUO Junlan, CHEN Bo, GUO Shengnan01. Department of Medical Oncology, Anyang Cancer Hospital Affiliated to Henan University of Science and Technology, Anyang 455000;;2. Department of Oncology, Guangdong Provincial People's Hospital Affiliated to Southern Medical University, Guangzhou 519041, ChinaObjective To investigate the expression of circular-RNA DDX5(circ-DDX5) in breast cancer tissues and its relationship with the clinical stage of breast cancer patients, and to analyze the regulatory mechanism of circ-DDX5 on the proliferation and invasion of human breast cancer cell line. Methods The expression level of circ-DDX5 in breast cancer tissues and its correlation with the clinical stage of breast cancer patients were analyzed by TCGA database. Bioinformatics analysis and dual-luciferase reporter gene experiments verified the targeting relationship between circ-DDX5 and miR-3940. The correlation between circ-DDX5 and miR-3940 expression in breast cancer tissues was analyzed by TCGA database. The expression level of circ-DDX5 in breast cancer SK-BR-3, MDA-MB-231, BT-549, MCF-7, and HCC-1937 cells was detected by RT-qPCR. The circ-DDX5 over-expression plasmid and negative control plasmid were transfected into MDA-MB-231 cells, which were named circ-DDX5 group and NC group, respectively. The proliferation and invasion of MDA-MB-231 cells in the circ-DDX5 group and the NC group were detected by colony formation assay and Transwell assay. The expressions of proliferation phenotype protein and invasion phenotype protein of MDA-MB-231 cells were detected by Western blot. The expression level of miR-3940 in MDA-MB-231 cells of circ-DDX5 group and NC group was detected by RT-qPCR. Results The expression of circ-DDX5 in breast cancer tissues was lower than that in adjacent tissues (P＜0.01) and the expression level of circ-DDX5 was negatively correlated with the clinical stage of breast cancer patients (P＜0.01). There was a targeting relationship between circ-DDX5 and miR-3940 (P＜0.01). The expression of circ-DDX5 and miR-3940 in breast cancer tissue was negatively correlated (P＜0.01). The expression of circ-DDX5 in human breast cancer cell lines was lower than that in immortalized breast epithelial cells MCF-10A (P＜0.05 or P＜0.01). Compared with the NC group, the over-expression of circ-DDX5 could significantly inhibit the proliferation and invasion of MDA-MB-231 cells(P＜0.01), as well as the proliferation phenotype proteins (cyclin C, CDK3) and invasion phenotype proteins (Snail, vimentin) expression (P＜0.01) and miR-3940 expression (P＜0.01). Conclusions The expression of circ-DDX5 in breast cancer tissues and cells is low. circ-DDX5 inhibits the proliferation and invasion of breast cancer MDA-MB-231 cells by targeting the expression of miR-3940.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2024-44-1-1.pdfbreast cancer|circular-rna ddx5|mir-3940|proliferation|invasion |
| spellingShingle | LI Jiangli, SUN Jing, TANG Yijun, GUO Junlan, CHEN Bo, GUO Shengnan Circ-DDX5 inhibits the proliferation and invasion of human breast cancer cell lines by targeting miR-3940 Jichu yixue yu linchuang breast cancer|circular-rna ddx5|mir-3940|proliferation|invasion |
| title | Circ-DDX5 inhibits the proliferation and invasion of human breast cancer cell lines by targeting miR-3940 |
| title_full | Circ-DDX5 inhibits the proliferation and invasion of human breast cancer cell lines by targeting miR-3940 |
| title_fullStr | Circ-DDX5 inhibits the proliferation and invasion of human breast cancer cell lines by targeting miR-3940 |
| title_full_unstemmed | Circ-DDX5 inhibits the proliferation and invasion of human breast cancer cell lines by targeting miR-3940 |
| title_short | Circ-DDX5 inhibits the proliferation and invasion of human breast cancer cell lines by targeting miR-3940 |
| title_sort | circ ddx5 inhibits the proliferation and invasion of human breast cancer cell lines by targeting mir 3940 |
| topic | breast cancer|circular-rna ddx5|mir-3940|proliferation|invasion |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2024-44-1-1.pdf |
| work_keys_str_mv | AT lijianglisunjingtangyijunguojunlanchenboguoshengnan circddx5inhibitstheproliferationandinvasionofhumanbreastcancercelllinesbytargetingmir3940 |