Development and Validation of a Rapid, Single-Step Reverse Transcriptase Loop-Mediated Isothermal Amplification (RT-LAMP) System Potentially to Be Used for Reliable and High-Throughput Screening of COVID-19

Development and Validation of a Rapid, Single-Step Reverse Transcriptase Loop-Mediated Isothermal Amplification (RT-LAMP) System Potentially to Be Used for Reliable and High-Throughput Screening of COVID-19

Objectives: Development and validation of a single-step and accurate reverse transcriptase loop-mediated isothermal amplification technique (RT-LAMP) for rapid identification of SARS-CoV-2 relative to commercial quantitative reverse transcriptase real-time PCR (qRT-PCR) assays to allow prompt initia...

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Bibliographic Details
Main Authors: Minghua Jiang, Weihua Pan, Amir Arasthfer, Wenjie Fang, Liyan Ling, Hua Fang, Farnaz Daneshnia, Jian Yu, Wanqing Liao, Hao Pei, Xiaojing Li, Cornelia Lass-Flörl
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-06-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
COVID-19
SARS-CoV-2
RT-LAMP
qRT-PCR
diagnostic test
Online Access:https://www.frontiersin.org/article/10.3389/fcimb.2020.00331/full

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