Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization
The efficient entry of nanotechnology-based pharmaceuticals into target cells is highly desired to reach high therapeutic efficiency while minimizing the side effects. Despite intensive research, the impact of the surface coating on the mechanism of nanoparticle uptake is not sufficiently understood...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Beilstein-Institut
2021-03-01
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| Series: | Beilstein Journal of Nanotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.3762/bjnano.12.22 |
| _version_ | 1819117754777600000 |
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| author | Barbora Svitkova Vlasta Zavisova Veronika Nemethova Martina Koneracka Miroslava Kretova Filip Razga Monika Ursinyova Alena Gabelova |
| author_facet | Barbora Svitkova Vlasta Zavisova Veronika Nemethova Martina Koneracka Miroslava Kretova Filip Razga Monika Ursinyova Alena Gabelova |
| author_sort | Barbora Svitkova |
| collection | DOAJ |
| description | The efficient entry of nanotechnology-based pharmaceuticals into target cells is highly desired to reach high therapeutic efficiency while minimizing the side effects. Despite intensive research, the impact of the surface coating on the mechanism of nanoparticle uptake is not sufficiently understood yet. Herein, we present a mechanistic study of cellular internalization pathways of two magnetic iron oxide nanoparticles (MNPs) differing in surface chemistry into A549 cells. The MNP uptake was investigated in the presence of different inhibitors of endocytosis and monitored by spectroscopic and imaging techniques. The results revealed that the route of MNP entry into cells strongly depends on the surface chemistry of the MNPs. While serum bovine albumin-coated MNPs entered the cells via clathrin-mediated endocytosis (CME), caveolin-mediated endocytosis (CavME) or lipid rafts were preferentially involved in the internalization of polyethylene glycol-coated MNPs. Our data indicate that surface engineering can contribute to an enhanced delivery efficiency of nanoparticles. |
| first_indexed | 2024-12-22T05:38:01Z |
| format | Article |
| id | doaj.art-e0ee84627c154f03a49004c5b8b2e5ba |
| institution | Directory Open Access Journal |
| issn | 2190-4286 |
| language | English |
| last_indexed | 2024-12-22T05:38:01Z |
| publishDate | 2021-03-01 |
| publisher | Beilstein-Institut |
| record_format | Article |
| series | Beilstein Journal of Nanotechnology |
| spelling | doaj.art-e0ee84627c154f03a49004c5b8b2e5ba2022-12-21T18:37:16ZengBeilstein-InstitutBeilstein Journal of Nanotechnology2190-42862021-03-0112127028110.3762/bjnano.12.222190-4286-12-22Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalizationBarbora Svitkova0Vlasta Zavisova1Veronika Nemethova2Martina Koneracka3Miroslava Kretova4Filip Razga5Monika Ursinyova6Alena Gabelova7Cancer Research Institute, Biomedical Research Center of the Slovak Academy of Sciences, Dubravska cesta 9, 845 05 Bratislava, SlovakiaInstitute of Experimental Physics, Slovak Academy of Sciences, Watsonova 47, 040 01 Kosice, SlovakiaFaculty of Medicine, Comenius University, Spitalska 24, 813 72 Bratislava, SlovakiaInstitute of Experimental Physics, Slovak Academy of Sciences, Watsonova 47, 040 01 Kosice, SlovakiaCancer Research Institute, Biomedical Research Center of the Slovak Academy of Sciences, Dubravska cesta 9, 845 05 Bratislava, SlovakiaFaculty of Medicine, Comenius University, Spitalska 24, 813 72 Bratislava, SlovakiaSlovak Medical University, Limbova 12, 833 03 Bratislava, SlovakiaCancer Research Institute, Biomedical Research Center of the Slovak Academy of Sciences, Dubravska cesta 9, 845 05 Bratislava, SlovakiaThe efficient entry of nanotechnology-based pharmaceuticals into target cells is highly desired to reach high therapeutic efficiency while minimizing the side effects. Despite intensive research, the impact of the surface coating on the mechanism of nanoparticle uptake is not sufficiently understood yet. Herein, we present a mechanistic study of cellular internalization pathways of two magnetic iron oxide nanoparticles (MNPs) differing in surface chemistry into A549 cells. The MNP uptake was investigated in the presence of different inhibitors of endocytosis and monitored by spectroscopic and imaging techniques. The results revealed that the route of MNP entry into cells strongly depends on the surface chemistry of the MNPs. While serum bovine albumin-coated MNPs entered the cells via clathrin-mediated endocytosis (CME), caveolin-mediated endocytosis (CavME) or lipid rafts were preferentially involved in the internalization of polyethylene glycol-coated MNPs. Our data indicate that surface engineering can contribute to an enhanced delivery efficiency of nanoparticles.https://doi.org/10.3762/bjnano.12.22bovine serum albumincellular uptakemagnetic iron oxide nanoparticlespolyethylene glycolsurface coating |
| spellingShingle | Barbora Svitkova Vlasta Zavisova Veronika Nemethova Martina Koneracka Miroslava Kretova Filip Razga Monika Ursinyova Alena Gabelova Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization Beilstein Journal of Nanotechnology bovine serum albumin cellular uptake magnetic iron oxide nanoparticles polyethylene glycol surface coating |
| title | Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization |
| title_full | Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization |
| title_fullStr | Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization |
| title_full_unstemmed | Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization |
| title_short | Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization |
| title_sort | differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization |
| topic | bovine serum albumin cellular uptake magnetic iron oxide nanoparticles polyethylene glycol surface coating |
| url | https://doi.org/10.3762/bjnano.12.22 |
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