Genome Sequencing and Comparative Analysis of Stenotrophomonas acidaminiphila Reveal Evolutionary Insights Into Sulfamethoxazole Resistance
Stenotrophomonas acidaminiphila is an aerobic, glucose non-fermentative, Gram-negative bacterium that been isolated from various environmental sources, particularly aquatic ecosystems. Although resistance to multiple antimicrobial agents has been reported in S. acidaminiphila, the mechanisms are lar...
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Frontiers Media S.A.
2018-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2018.01013/full |
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| author | Yao-Ting Huang Jia-Min Chen Bing-Ching Ho Zong-Yen Wu Zong-Yen Wu Rita C. Kuo Po-Yu Liu Po-Yu Liu Po-Yu Liu |
| author_facet | Yao-Ting Huang Jia-Min Chen Bing-Ching Ho Zong-Yen Wu Zong-Yen Wu Rita C. Kuo Po-Yu Liu Po-Yu Liu Po-Yu Liu |
| author_sort | Yao-Ting Huang |
| collection | DOAJ |
| description | Stenotrophomonas acidaminiphila is an aerobic, glucose non-fermentative, Gram-negative bacterium that been isolated from various environmental sources, particularly aquatic ecosystems. Although resistance to multiple antimicrobial agents has been reported in S. acidaminiphila, the mechanisms are largely unknown. Here, for the first time, we report the complete genome and antimicrobial resistome analysis of a clinical isolate S. acidaminiphila SUNEO which is resistant to sulfamethoxazole. Comparative analysis among closely related strains identified common and strain-specific genes. In particular, comparison with a sulfamethoxazole-sensitive strain identified a mutation within the sulfonamide-binding site of folP in SUNEO, which may reduce the binding affinity of sulfamethoxazole. Selection pressure analysis indicated folP in SUNEO is under purifying selection, which may be owing to long-term administration of sulfonamide against Stenotrophomonas. |
| first_indexed | 2024-12-19T03:00:45Z |
| format | Article |
| id | doaj.art-e0ee9c9913194b7cb12cf07427a68836 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-19T03:00:45Z |
| publishDate | 2018-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-e0ee9c9913194b7cb12cf07427a688362022-12-21T20:38:13ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-05-01910.3389/fmicb.2018.01013368992Genome Sequencing and Comparative Analysis of Stenotrophomonas acidaminiphila Reveal Evolutionary Insights Into Sulfamethoxazole ResistanceYao-Ting Huang0Jia-Min Chen1Bing-Ching Ho2Zong-Yen Wu3Zong-Yen Wu4Rita C. Kuo5Po-Yu Liu6Po-Yu Liu7Po-Yu Liu8Department of Computer Science and Information Engineering, National Chung Cheng University, Chiayi, TaiwanDepartment of Computer Science and Information Engineering, National Chung Cheng University, Chiayi, TaiwanDepartment of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University Hospital, Taipei, TaiwanDOE Joint Genome Institute, Walnut Creek, CA, United StatesDepartment of Veterinary Medicine, National Chung Hsing University, Taichung, TaiwanDOE Joint Genome Institute, Walnut Creek, CA, United StatesThe Department of Nursing, Shu-Zen Junior College of Medicine and Management, Kaohsiung, TaiwanRong Hsing Research Center for Translational Medicine, College of Life Sciences, National Chung Hsing University, Taichung, TaiwanDivision of Infectious Diseases, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung, TaiwanStenotrophomonas acidaminiphila is an aerobic, glucose non-fermentative, Gram-negative bacterium that been isolated from various environmental sources, particularly aquatic ecosystems. Although resistance to multiple antimicrobial agents has been reported in S. acidaminiphila, the mechanisms are largely unknown. Here, for the first time, we report the complete genome and antimicrobial resistome analysis of a clinical isolate S. acidaminiphila SUNEO which is resistant to sulfamethoxazole. Comparative analysis among closely related strains identified common and strain-specific genes. In particular, comparison with a sulfamethoxazole-sensitive strain identified a mutation within the sulfonamide-binding site of folP in SUNEO, which may reduce the binding affinity of sulfamethoxazole. Selection pressure analysis indicated folP in SUNEO is under purifying selection, which may be owing to long-term administration of sulfonamide against Stenotrophomonas.http://journal.frontiersin.org/article/10.3389/fmicb.2018.01013/fullgenome sequencingStenotrophomonas acidaminiphilasulfamethoxazole resistanceStenotrophomonascomparative genomicsdihydropteroate synthase |
| spellingShingle | Yao-Ting Huang Jia-Min Chen Bing-Ching Ho Zong-Yen Wu Zong-Yen Wu Rita C. Kuo Po-Yu Liu Po-Yu Liu Po-Yu Liu Genome Sequencing and Comparative Analysis of Stenotrophomonas acidaminiphila Reveal Evolutionary Insights Into Sulfamethoxazole Resistance Frontiers in Microbiology genome sequencing Stenotrophomonas acidaminiphila sulfamethoxazole resistance Stenotrophomonas comparative genomics dihydropteroate synthase |
| title | Genome Sequencing and Comparative Analysis of Stenotrophomonas acidaminiphila Reveal Evolutionary Insights Into Sulfamethoxazole Resistance |
| title_full | Genome Sequencing and Comparative Analysis of Stenotrophomonas acidaminiphila Reveal Evolutionary Insights Into Sulfamethoxazole Resistance |
| title_fullStr | Genome Sequencing and Comparative Analysis of Stenotrophomonas acidaminiphila Reveal Evolutionary Insights Into Sulfamethoxazole Resistance |
| title_full_unstemmed | Genome Sequencing and Comparative Analysis of Stenotrophomonas acidaminiphila Reveal Evolutionary Insights Into Sulfamethoxazole Resistance |
| title_short | Genome Sequencing and Comparative Analysis of Stenotrophomonas acidaminiphila Reveal Evolutionary Insights Into Sulfamethoxazole Resistance |
| title_sort | genome sequencing and comparative analysis of stenotrophomonas acidaminiphila reveal evolutionary insights into sulfamethoxazole resistance |
| topic | genome sequencing Stenotrophomonas acidaminiphila sulfamethoxazole resistance Stenotrophomonas comparative genomics dihydropteroate synthase |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2018.01013/full |
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