Comparison of two porcine-derived materials for repairing abdominal wall defects in rats.
OBJECTIVE: The purpose of this study was to compare the mechanical properties, host responses and incorporation of porcine small intestine submucosa (PSIS) and porcine acellular dermal matrix (PADM) in a rat model of abdominal wall defect repair. MATERIALS AND METHODS: Prior to implantation, PSIS an...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3102734?pdf=render |
| _version_ | 1818327561336258560 |
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| author | Zhengni Liu Rui Tang Zhiyuan Zhou Zhicheng Song Huichun Wang Yan Gu |
| author_facet | Zhengni Liu Rui Tang Zhiyuan Zhou Zhicheng Song Huichun Wang Yan Gu |
| author_sort | Zhengni Liu |
| collection | DOAJ |
| description | OBJECTIVE: The purpose of this study was to compare the mechanical properties, host responses and incorporation of porcine small intestine submucosa (PSIS) and porcine acellular dermal matrix (PADM) in a rat model of abdominal wall defect repair. MATERIALS AND METHODS: Prior to implantation, PSIS and PADM were prepared and evaluated in terms of structure and mechanical properties. Full-thickness abdominal wall defects were created in 50 Sprague-Dawley rats, and were repaired using either PSIS or PADM. Rats were sacrificed 1, 2, 4, 8 and 12 weeks post-repair and examined for herniation, infection, adhesions, contraction, and changes in the thickness and strength of the tissues incorporated at the defect sites. Histopathology and immunohistochemistry were performed to analyze inflammatory responses, collagen deposition and vascularization. RESULTS: PADM showed more dense collagen deposition and stronger mechanical properties than PSIS prior to implantation (P<0.01). However, the mechanical properties observed after integration with the surrounding native tissues was similar for PADM and PSIS. Both PADM and PSIS showed significant contraction by week 12. However, PADM tissue induced less adhesion and increased in thickness more slowly, and showed less infiltration by foreign giant cells, polymorphonuclear cells, and mononuclear cells. Improved remodeling of host tissue was observed after PSIS implantation, which was apparent from the orientation of bands of fibrous connective tissue, intermixed with newly formed blood vessels by Week 12. CONCLUSION: PSIS showed weaker mechanical properties prior to implantation. However, after implantation PSIS induced more pronounced host responses and showed better incorporation into host tissues than PADM. |
| first_indexed | 2024-12-13T12:18:14Z |
| format | Article |
| id | doaj.art-e0f155ae14704aae8313b0cb607c5434 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T12:18:14Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e0f155ae14704aae8313b0cb607c54342022-12-21T23:46:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0165e2052010.1371/journal.pone.0020520Comparison of two porcine-derived materials for repairing abdominal wall defects in rats.Zhengni LiuRui TangZhiyuan ZhouZhicheng SongHuichun WangYan GuOBJECTIVE: The purpose of this study was to compare the mechanical properties, host responses and incorporation of porcine small intestine submucosa (PSIS) and porcine acellular dermal matrix (PADM) in a rat model of abdominal wall defect repair. MATERIALS AND METHODS: Prior to implantation, PSIS and PADM were prepared and evaluated in terms of structure and mechanical properties. Full-thickness abdominal wall defects were created in 50 Sprague-Dawley rats, and were repaired using either PSIS or PADM. Rats were sacrificed 1, 2, 4, 8 and 12 weeks post-repair and examined for herniation, infection, adhesions, contraction, and changes in the thickness and strength of the tissues incorporated at the defect sites. Histopathology and immunohistochemistry were performed to analyze inflammatory responses, collagen deposition and vascularization. RESULTS: PADM showed more dense collagen deposition and stronger mechanical properties than PSIS prior to implantation (P<0.01). However, the mechanical properties observed after integration with the surrounding native tissues was similar for PADM and PSIS. Both PADM and PSIS showed significant contraction by week 12. However, PADM tissue induced less adhesion and increased in thickness more slowly, and showed less infiltration by foreign giant cells, polymorphonuclear cells, and mononuclear cells. Improved remodeling of host tissue was observed after PSIS implantation, which was apparent from the orientation of bands of fibrous connective tissue, intermixed with newly formed blood vessels by Week 12. CONCLUSION: PSIS showed weaker mechanical properties prior to implantation. However, after implantation PSIS induced more pronounced host responses and showed better incorporation into host tissues than PADM.http://europepmc.org/articles/PMC3102734?pdf=render |
| spellingShingle | Zhengni Liu Rui Tang Zhiyuan Zhou Zhicheng Song Huichun Wang Yan Gu Comparison of two porcine-derived materials for repairing abdominal wall defects in rats. PLoS ONE |
| title | Comparison of two porcine-derived materials for repairing abdominal wall defects in rats. |
| title_full | Comparison of two porcine-derived materials for repairing abdominal wall defects in rats. |
| title_fullStr | Comparison of two porcine-derived materials for repairing abdominal wall defects in rats. |
| title_full_unstemmed | Comparison of two porcine-derived materials for repairing abdominal wall defects in rats. |
| title_short | Comparison of two porcine-derived materials for repairing abdominal wall defects in rats. |
| title_sort | comparison of two porcine derived materials for repairing abdominal wall defects in rats |
| url | http://europepmc.org/articles/PMC3102734?pdf=render |
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