Diversity and Ecology of Marine Algicolous <i>Arthrinium</i> Species as a Source of Bioactive Natural Products
In our previous study, all <i>Arthrinium</i> isolates from <i>Sargassum</i> sp. showed high bioactivities, but studies on marine <i>Arthrinium</i> spp. are insufficient. In this study, a phylogenetic analysis of 28 <i>Arthrinium</i> isolates from seawe...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-12-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/16/12/508 |
| _version_ | 1798026947533471744 |
|---|---|
| author | Young Mok Heo Kyeongwon Kim Seung Mok Ryu Sun Lul Kwon Min Young Park Ji Eun Kang Joo-Hyun Hong Young Woon Lim Changmu Kim Beom Seok Kim Dongho Lee Jae-Jin Kim |
| author_facet | Young Mok Heo Kyeongwon Kim Seung Mok Ryu Sun Lul Kwon Min Young Park Ji Eun Kang Joo-Hyun Hong Young Woon Lim Changmu Kim Beom Seok Kim Dongho Lee Jae-Jin Kim |
| author_sort | Young Mok Heo |
| collection | DOAJ |
| description | In our previous study, all <i>Arthrinium</i> isolates from <i>Sargassum</i> sp. showed high bioactivities, but studies on marine <i>Arthrinium</i> spp. are insufficient. In this study, a phylogenetic analysis of 28 <i>Arthrinium</i> isolates from seaweeds and egg masses of <i>Arctoscopus japonicus</i> was conducted using internal transcribed spacers, nuclear large subunit rDNA, β-tubulin, and translation elongation factor region sequences, and their bioactivities were investigated. They were analyzed as 15 species, and 11 of them were found to be new species. Most of the extracts exhibited radical-scavenging activity, and some showed antifungal activities, tyrosinase inhibition, and quorum sensing inhibition. It was implied that marine algicolous <i>Arthrinium</i> spp. support the regulation of reactive oxygen species in symbiotic algae and protect against pathogens and bacterial biofilm formation. The antioxidant from <i>Arthrinium</i> sp. 10 KUC21332 was separated by bioassay-guided isolation and identified to be gentisyl alcohol, and the antioxidant of <i>Arthrinium saccharicola</i> KUC21221 was identical. These results demonstrate that many unexploited <i>Arthrinium</i> species still exist in marine environments and that they are a great source of bioactive compounds. |
| first_indexed | 2024-04-11T18:43:31Z |
| format | Article |
| id | doaj.art-e0f3025f59464753bbc6ff6983770f3d |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T18:43:31Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-e0f3025f59464753bbc6ff6983770f3d2022-12-22T04:08:54ZengMDPI AGMarine Drugs1660-33972018-12-01161250810.3390/md16120508md16120508Diversity and Ecology of Marine Algicolous <i>Arthrinium</i> Species as a Source of Bioactive Natural ProductsYoung Mok Heo0Kyeongwon Kim1Seung Mok Ryu2Sun Lul Kwon3Min Young Park4Ji Eun Kang5Joo-Hyun Hong6Young Woon Lim7Changmu Kim8Beom Seok Kim9Dongho Lee10Jae-Jin Kim11Division of Environmental Science & Ecological Engineering, College of Life Sciences & Biotechnology, Korea University, Seoul 02841, KoreaDivision of Environmental Science & Ecological Engineering, College of Life Sciences & Biotechnology, Korea University, Seoul 02841, KoreaDivision of Biotechnology, College of Life Sciences & Biotechnology, Korea University, Seoul 02841, KoreaDivision of Environmental Science & Ecological Engineering, College of Life Sciences & Biotechnology, Korea University, Seoul 02841, KoreaDivision of Biotechnology, College of Life Sciences & Biotechnology, Korea University, Seoul 02841, KoreaDivision of Biotechnology, College of Life Sciences & Biotechnology, Korea University, Seoul 02841, KoreaDivision of Environmental Science & Ecological Engineering, College of Life Sciences & Biotechnology, Korea University, Seoul 02841, KoreaSchool of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 08826, KoreaMicroorganism Resources Division, National Institute of Biological Resources, Incheon 22689, KoreaDivision of Biotechnology, College of Life Sciences & Biotechnology, Korea University, Seoul 02841, KoreaDivision of Biotechnology, College of Life Sciences & Biotechnology, Korea University, Seoul 02841, KoreaDivision of Environmental Science & Ecological Engineering, College of Life Sciences & Biotechnology, Korea University, Seoul 02841, KoreaIn our previous study, all <i>Arthrinium</i> isolates from <i>Sargassum</i> sp. showed high bioactivities, but studies on marine <i>Arthrinium</i> spp. are insufficient. In this study, a phylogenetic analysis of 28 <i>Arthrinium</i> isolates from seaweeds and egg masses of <i>Arctoscopus japonicus</i> was conducted using internal transcribed spacers, nuclear large subunit rDNA, β-tubulin, and translation elongation factor region sequences, and their bioactivities were investigated. They were analyzed as 15 species, and 11 of them were found to be new species. Most of the extracts exhibited radical-scavenging activity, and some showed antifungal activities, tyrosinase inhibition, and quorum sensing inhibition. It was implied that marine algicolous <i>Arthrinium</i> spp. support the regulation of reactive oxygen species in symbiotic algae and protect against pathogens and bacterial biofilm formation. The antioxidant from <i>Arthrinium</i> sp. 10 KUC21332 was separated by bioassay-guided isolation and identified to be gentisyl alcohol, and the antioxidant of <i>Arthrinium saccharicola</i> KUC21221 was identical. These results demonstrate that many unexploited <i>Arthrinium</i> species still exist in marine environments and that they are a great source of bioactive compounds.https://www.mdpi.com/1660-3397/16/12/508antioxidantbiological controlecological rolegentisyl alcoholmulti-gene phylogenytyrosinase inhibition |
| spellingShingle | Young Mok Heo Kyeongwon Kim Seung Mok Ryu Sun Lul Kwon Min Young Park Ji Eun Kang Joo-Hyun Hong Young Woon Lim Changmu Kim Beom Seok Kim Dongho Lee Jae-Jin Kim Diversity and Ecology of Marine Algicolous <i>Arthrinium</i> Species as a Source of Bioactive Natural Products Marine Drugs antioxidant biological control ecological role gentisyl alcohol multi-gene phylogeny tyrosinase inhibition |
| title | Diversity and Ecology of Marine Algicolous <i>Arthrinium</i> Species as a Source of Bioactive Natural Products |
| title_full | Diversity and Ecology of Marine Algicolous <i>Arthrinium</i> Species as a Source of Bioactive Natural Products |
| title_fullStr | Diversity and Ecology of Marine Algicolous <i>Arthrinium</i> Species as a Source of Bioactive Natural Products |
| title_full_unstemmed | Diversity and Ecology of Marine Algicolous <i>Arthrinium</i> Species as a Source of Bioactive Natural Products |
| title_short | Diversity and Ecology of Marine Algicolous <i>Arthrinium</i> Species as a Source of Bioactive Natural Products |
| title_sort | diversity and ecology of marine algicolous i arthrinium i species as a source of bioactive natural products |
| topic | antioxidant biological control ecological role gentisyl alcohol multi-gene phylogeny tyrosinase inhibition |
| url | https://www.mdpi.com/1660-3397/16/12/508 |
| work_keys_str_mv | AT youngmokheo diversityandecologyofmarinealgicolousiarthriniumispeciesasasourceofbioactivenaturalproducts AT kyeongwonkim diversityandecologyofmarinealgicolousiarthriniumispeciesasasourceofbioactivenaturalproducts AT seungmokryu diversityandecologyofmarinealgicolousiarthriniumispeciesasasourceofbioactivenaturalproducts AT sunlulkwon diversityandecologyofmarinealgicolousiarthriniumispeciesasasourceofbioactivenaturalproducts AT minyoungpark diversityandecologyofmarinealgicolousiarthriniumispeciesasasourceofbioactivenaturalproducts AT jieunkang diversityandecologyofmarinealgicolousiarthriniumispeciesasasourceofbioactivenaturalproducts AT joohyunhong diversityandecologyofmarinealgicolousiarthriniumispeciesasasourceofbioactivenaturalproducts AT youngwoonlim diversityandecologyofmarinealgicolousiarthriniumispeciesasasourceofbioactivenaturalproducts AT changmukim diversityandecologyofmarinealgicolousiarthriniumispeciesasasourceofbioactivenaturalproducts AT beomseokkim diversityandecologyofmarinealgicolousiarthriniumispeciesasasourceofbioactivenaturalproducts AT dongholee diversityandecologyofmarinealgicolousiarthriniumispeciesasasourceofbioactivenaturalproducts AT jaejinkim diversityandecologyofmarinealgicolousiarthriniumispeciesasasourceofbioactivenaturalproducts |