Efficient molasses utilization for low-molecular-weight poly-γ-glutamic acid production using a novel Bacillus subtilis stain
Abstract Background Poly-γ-glutamic acid (γ-PGA) is a biopolymer and has various applications based on its biocompatibility, non-toxicity, and edibility. Low-molecular-weight (Mw)-γ-PGA has promising applications in agriculture and pharmaceuticals. It is traditionally produced by enzymatic hydrolysi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-07-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-022-01867-5 |
| _version_ | 1811293426214240256 |
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| author | Jing Li Shengbao Chen Jiaming Fu Jianchun Xie Jiansong Ju Bo Yu Limin Wang |
| author_facet | Jing Li Shengbao Chen Jiaming Fu Jianchun Xie Jiansong Ju Bo Yu Limin Wang |
| author_sort | Jing Li |
| collection | DOAJ |
| description | Abstract Background Poly-γ-glutamic acid (γ-PGA) is a biopolymer and has various applications based on its biocompatibility, non-toxicity, and edibility. Low-molecular-weight (Mw)-γ-PGA has promising applications in agriculture and pharmaceuticals. It is traditionally produced by enzymatic hydrolysis. Cost-effective bioproduction of low-Mw-γ-PGA is essential for commercial application of γ-PGA. Results Bacillus subtilis 242 is a newly isolated low-Mw-γ-PGA-producing strain. To develop cost-effective production of γ-PGA using this newly isolated strain, cane molasses and corn steep liquor were used to produce γ-PGA. The concentration of cane molasses was optimized and 100 g/L cane molasses resulted in high γ-PGA production. The effects of yeast extract and corn steep liquor on γ-PGA yield were investigated. High concentration of γ-PGA was obtained in the medium with corn steep liquor. A concentration of 32.14 g/L γ-PGA was achieved in fed-batch fermentation, with a productivity of 0.67 g/L/h and a percentage yield (g γ-PGA/g glutamate) of 106.39%. The Mw of γ-PGA was 27.99 kDa. Conclusion This study demonstrated the potential application of B. subtilis 242 for cost-effective production of low-Mw-γ-PGA from cane molasses. |
| first_indexed | 2024-04-13T05:01:13Z |
| format | Article |
| id | doaj.art-e0f61221b26242e599c81ed716693639 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-04-13T05:01:13Z |
| publishDate | 2022-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-e0f61221b26242e599c81ed7166936392022-12-22T03:01:19ZengBMCMicrobial Cell Factories1475-28592022-07-012111910.1186/s12934-022-01867-5Efficient molasses utilization for low-molecular-weight poly-γ-glutamic acid production using a novel Bacillus subtilis stainJing Li0Shengbao Chen1Jiaming Fu2Jianchun Xie3Jiansong Ju4Bo Yu5Limin Wang6Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU)CAS Key Laboratory of Microbial Physiological & Metabolic Engineering, State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of SciencesCollege of Chemical Engineering, Nanjing Forestry UniversityBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU)College of Life Science, Hebei Normal UniversityCAS Key Laboratory of Microbial Physiological & Metabolic Engineering, State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of SciencesBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU)Abstract Background Poly-γ-glutamic acid (γ-PGA) is a biopolymer and has various applications based on its biocompatibility, non-toxicity, and edibility. Low-molecular-weight (Mw)-γ-PGA has promising applications in agriculture and pharmaceuticals. It is traditionally produced by enzymatic hydrolysis. Cost-effective bioproduction of low-Mw-γ-PGA is essential for commercial application of γ-PGA. Results Bacillus subtilis 242 is a newly isolated low-Mw-γ-PGA-producing strain. To develop cost-effective production of γ-PGA using this newly isolated strain, cane molasses and corn steep liquor were used to produce γ-PGA. The concentration of cane molasses was optimized and 100 g/L cane molasses resulted in high γ-PGA production. The effects of yeast extract and corn steep liquor on γ-PGA yield were investigated. High concentration of γ-PGA was obtained in the medium with corn steep liquor. A concentration of 32.14 g/L γ-PGA was achieved in fed-batch fermentation, with a productivity of 0.67 g/L/h and a percentage yield (g γ-PGA/g glutamate) of 106.39%. The Mw of γ-PGA was 27.99 kDa. Conclusion This study demonstrated the potential application of B. subtilis 242 for cost-effective production of low-Mw-γ-PGA from cane molasses.https://doi.org/10.1186/s12934-022-01867-5Poly-γ-glutamic acidBacillus subtilisCane molassesLow molecular weight |
| spellingShingle | Jing Li Shengbao Chen Jiaming Fu Jianchun Xie Jiansong Ju Bo Yu Limin Wang Efficient molasses utilization for low-molecular-weight poly-γ-glutamic acid production using a novel Bacillus subtilis stain Microbial Cell Factories Poly-γ-glutamic acid Bacillus subtilis Cane molasses Low molecular weight |
| title | Efficient molasses utilization for low-molecular-weight poly-γ-glutamic acid production using a novel Bacillus subtilis stain |
| title_full | Efficient molasses utilization for low-molecular-weight poly-γ-glutamic acid production using a novel Bacillus subtilis stain |
| title_fullStr | Efficient molasses utilization for low-molecular-weight poly-γ-glutamic acid production using a novel Bacillus subtilis stain |
| title_full_unstemmed | Efficient molasses utilization for low-molecular-weight poly-γ-glutamic acid production using a novel Bacillus subtilis stain |
| title_short | Efficient molasses utilization for low-molecular-weight poly-γ-glutamic acid production using a novel Bacillus subtilis stain |
| title_sort | efficient molasses utilization for low molecular weight poly γ glutamic acid production using a novel bacillus subtilis stain |
| topic | Poly-γ-glutamic acid Bacillus subtilis Cane molasses Low molecular weight |
| url | https://doi.org/10.1186/s12934-022-01867-5 |
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