Advancements in Reference Gene Selection for Fruit Trees: A Comprehensive Review
Real-time quantitative polymerase chain reaction (qRT-PCR) has been widely used in gene expression analyses due to its advantages of sensitivity, accuracy and high throughput. The stability of internal reference genes has progressively emerged as a major factor affecting the precision of qRT-PCR res...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/2/1142 |
| _version_ | 1797339778922840064 |
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| author | Shujun Peng Irfan Ali Sabir Xinglong Hu Jiayi Chen Yonghua Qin |
| author_facet | Shujun Peng Irfan Ali Sabir Xinglong Hu Jiayi Chen Yonghua Qin |
| author_sort | Shujun Peng |
| collection | DOAJ |
| description | Real-time quantitative polymerase chain reaction (qRT-PCR) has been widely used in gene expression analyses due to its advantages of sensitivity, accuracy and high throughput. The stability of internal reference genes has progressively emerged as a major factor affecting the precision of qRT-PCR results. However, the stability of the expression of the reference genes needs to be determined further in different cells or organs, physiological and experimental conditions. Methods for evaluating these candidate internal reference genes have also evolved from simple single software evaluation to more reliable and accurate internal reference gene evaluation by combining different software tools in a comprehensive analysis. This study intends to provide a definitive reference for upcoming research that will be conducted on fruit trees. The primary focus of this review is to summarize the research progress in recent years regarding the selection and stability analysis of candidate reference genes for different fruit trees. |
| first_indexed | 2024-03-08T09:53:20Z |
| format | Article |
| id | doaj.art-e0fc98cd6afa4a64bd31e39231746e6b |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T09:53:20Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e0fc98cd6afa4a64bd31e39231746e6b2024-01-29T13:57:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-01-01252114210.3390/ijms25021142Advancements in Reference Gene Selection for Fruit Trees: A Comprehensive ReviewShujun Peng0Irfan Ali Sabir1Xinglong Hu2Jiayi Chen3Yonghua Qin4Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaReal-time quantitative polymerase chain reaction (qRT-PCR) has been widely used in gene expression analyses due to its advantages of sensitivity, accuracy and high throughput. The stability of internal reference genes has progressively emerged as a major factor affecting the precision of qRT-PCR results. However, the stability of the expression of the reference genes needs to be determined further in different cells or organs, physiological and experimental conditions. Methods for evaluating these candidate internal reference genes have also evolved from simple single software evaluation to more reliable and accurate internal reference gene evaluation by combining different software tools in a comprehensive analysis. This study intends to provide a definitive reference for upcoming research that will be conducted on fruit trees. The primary focus of this review is to summarize the research progress in recent years regarding the selection and stability analysis of candidate reference genes for different fruit trees.https://www.mdpi.com/1422-0067/25/2/1142fruit treesreference genesqRT-PCR |
| spellingShingle | Shujun Peng Irfan Ali Sabir Xinglong Hu Jiayi Chen Yonghua Qin Advancements in Reference Gene Selection for Fruit Trees: A Comprehensive Review International Journal of Molecular Sciences fruit trees reference genes qRT-PCR |
| title | Advancements in Reference Gene Selection for Fruit Trees: A Comprehensive Review |
| title_full | Advancements in Reference Gene Selection for Fruit Trees: A Comprehensive Review |
| title_fullStr | Advancements in Reference Gene Selection for Fruit Trees: A Comprehensive Review |
| title_full_unstemmed | Advancements in Reference Gene Selection for Fruit Trees: A Comprehensive Review |
| title_short | Advancements in Reference Gene Selection for Fruit Trees: A Comprehensive Review |
| title_sort | advancements in reference gene selection for fruit trees a comprehensive review |
| topic | fruit trees reference genes qRT-PCR |
| url | https://www.mdpi.com/1422-0067/25/2/1142 |
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