20-hydroxyecdysone reprograms amino acid metabolism to support the metamorphic development of Helicoverpa armigera
Summary: Amino acid metabolism is regulated according to nutrient conditions; however, the mechanism is not fully understood. Using the holometabolous insect cotton bollworm (Helicoverpa armigera) as a model, we report that hemolymph metabolites are greatly changed from the feeding larvae to the wan...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124723006551 |
| _version_ | 1797805858636169216 |
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| author | Xiao-Pei Wang Shu-Peng Sun Yan-Xue Li Lin Wang Du-Juan Dong Jin-Xing Wang Xiao-Fan Zhao |
| author_facet | Xiao-Pei Wang Shu-Peng Sun Yan-Xue Li Lin Wang Du-Juan Dong Jin-Xing Wang Xiao-Fan Zhao |
| author_sort | Xiao-Pei Wang |
| collection | DOAJ |
| description | Summary: Amino acid metabolism is regulated according to nutrient conditions; however, the mechanism is not fully understood. Using the holometabolous insect cotton bollworm (Helicoverpa armigera) as a model, we report that hemolymph metabolites are greatly changed from the feeding larvae to the wandering larvae and to pupae. Arginine, alpha-ketoglutarate (α-KG), and glutamate (Glu) are identified as marker metabolites of feeding larvae, wandering larvae, and pupae, respectively. Arginine level is decreased by 20-hydroxyecdysone (20E) regulation via repression of argininosuccinate synthetase (Ass) expression and upregulation of arginase (Arg) expression during metamorphosis. α-KG is transformed from Glu by glutamate dehydrogenase (GDH) in larval midgut, which is repressed by 20E. The α-KG is then transformed to Glu by GDH-like in pupal fat body, which is upregulated by 20E. Thus, 20E reprogrammed amino acid metabolism during metamorphosis by regulating gene expression in a stage- and tissue-specific manner to support insect metamorphic development. |
| first_indexed | 2024-03-13T05:58:27Z |
| format | Article |
| id | doaj.art-e0ff134b795549d5bc4548ca4b03ecf1 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-03-13T05:58:27Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-e0ff134b795549d5bc4548ca4b03ecf12023-06-13T04:12:04ZengElsevierCell Reports2211-12472023-06-0142611264420-hydroxyecdysone reprograms amino acid metabolism to support the metamorphic development of Helicoverpa armigeraXiao-Pei Wang0Shu-Peng Sun1Yan-Xue Li2Lin Wang3Du-Juan Dong4Jin-Xing Wang5Xiao-Fan Zhao6Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao 266237, ChinaShandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao 266237, ChinaShandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao 266237, ChinaShandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao 266237, ChinaShandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao 266237, ChinaShandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao 266237, ChinaShandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao 266237, China; Corresponding authorSummary: Amino acid metabolism is regulated according to nutrient conditions; however, the mechanism is not fully understood. Using the holometabolous insect cotton bollworm (Helicoverpa armigera) as a model, we report that hemolymph metabolites are greatly changed from the feeding larvae to the wandering larvae and to pupae. Arginine, alpha-ketoglutarate (α-KG), and glutamate (Glu) are identified as marker metabolites of feeding larvae, wandering larvae, and pupae, respectively. Arginine level is decreased by 20-hydroxyecdysone (20E) regulation via repression of argininosuccinate synthetase (Ass) expression and upregulation of arginase (Arg) expression during metamorphosis. α-KG is transformed from Glu by glutamate dehydrogenase (GDH) in larval midgut, which is repressed by 20E. The α-KG is then transformed to Glu by GDH-like in pupal fat body, which is upregulated by 20E. Thus, 20E reprogrammed amino acid metabolism during metamorphosis by regulating gene expression in a stage- and tissue-specific manner to support insect metamorphic development.http://www.sciencedirect.com/science/article/pii/S2211124723006551CP: MetabolismCP: Developmental biology |
| spellingShingle | Xiao-Pei Wang Shu-Peng Sun Yan-Xue Li Lin Wang Du-Juan Dong Jin-Xing Wang Xiao-Fan Zhao 20-hydroxyecdysone reprograms amino acid metabolism to support the metamorphic development of Helicoverpa armigera Cell Reports CP: Metabolism CP: Developmental biology |
| title | 20-hydroxyecdysone reprograms amino acid metabolism to support the metamorphic development of Helicoverpa armigera |
| title_full | 20-hydroxyecdysone reprograms amino acid metabolism to support the metamorphic development of Helicoverpa armigera |
| title_fullStr | 20-hydroxyecdysone reprograms amino acid metabolism to support the metamorphic development of Helicoverpa armigera |
| title_full_unstemmed | 20-hydroxyecdysone reprograms amino acid metabolism to support the metamorphic development of Helicoverpa armigera |
| title_short | 20-hydroxyecdysone reprograms amino acid metabolism to support the metamorphic development of Helicoverpa armigera |
| title_sort | 20 hydroxyecdysone reprograms amino acid metabolism to support the metamorphic development of helicoverpa armigera |
| topic | CP: Metabolism CP: Developmental biology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124723006551 |
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