Dual-targeted nano-encapsulation of neonatal porcine islet-like cell clusters with triiodothyronine-loaded bifunctional polymersomes
Abstract There is growing evidence that neonatal porcine islet-like cell clusters (NPCCs) isolated from piglets can be used to treat type 1 diabetes in humans. However, graft rejection is a common complication in humans owing to the prevalence of xenoantigens in porcine. Therefore, researchers have...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2024-02-01
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| Series: | Discover Nano |
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| Online Access: | https://doi.org/10.1186/s11671-024-03964-3 |
| _version_ | 1797273561402966016 |
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| author | Sang Hoon Lee Minse Kim Eun-Jin Lee Sun Mi Ahn Yu-Rim Ahn Jaewon Choi Jung-Taek Kang Hyun-Ouk Kim |
| author_facet | Sang Hoon Lee Minse Kim Eun-Jin Lee Sun Mi Ahn Yu-Rim Ahn Jaewon Choi Jung-Taek Kang Hyun-Ouk Kim |
| author_sort | Sang Hoon Lee |
| collection | DOAJ |
| description | Abstract There is growing evidence that neonatal porcine islet-like cell clusters (NPCCs) isolated from piglets can be used to treat type 1 diabetes in humans. However, graft rejection is a common complication in humans owing to the prevalence of xenoantigens in porcine. Therefore, researchers have investigated various islet encapsulation techniques that could protect against these antigens. To this end, this study presents a robust nano-encapsulation method based on bifunctional polymersomes (PSomes), in which N-hydroxysuccinimide (NHS) and maleimide (Mal) groups conjugated to the PSomes terminal interact with the amine and thiol groups on the surface of NPCCs to induce dual targeting via two covalent bonds. The findings indicate that the ratio of NHS to Mal on PSomes is optimal for dual targeting. Moreover, triiodothyronine (T3) is known to promotes pancreatic islet maturation and differentiation of endocrine cells into beta cells. T3 encapsulated in PSomes is shown to increase the glucose sensitivity of NPCCs and enhance insulin secretion from NPCCs. Furthermore, improvements in the nano-encapsulation efficiency and insulin-secreting capability of NPCCs through dual targeting via dual-Psomes are demonstrated. In conclusion, the proposed nano-encapsulation technique could pave the way for significant advances in islet nano-encapsulation and the imprevement of NPCC immaturity via T3 release. |
| first_indexed | 2024-03-07T14:46:09Z |
| format | Article |
| id | doaj.art-e0ffdc5e4e7e419aa8a69ccf04e2604c |
| institution | Directory Open Access Journal |
| issn | 2731-9229 |
| language | English |
| last_indexed | 2024-03-07T14:46:09Z |
| publishDate | 2024-02-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Nano |
| spelling | doaj.art-e0ffdc5e4e7e419aa8a69ccf04e2604c2024-03-05T20:02:02ZengSpringerDiscover Nano2731-92292024-02-0119111210.1186/s11671-024-03964-3Dual-targeted nano-encapsulation of neonatal porcine islet-like cell clusters with triiodothyronine-loaded bifunctional polymersomesSang Hoon Lee0Minse Kim1Eun-Jin Lee2Sun Mi Ahn3Yu-Rim Ahn4Jaewon Choi5Jung-Taek Kang6Hyun-Ouk Kim7MGENSolutions Biotechnology Research InstituteDepartment of Biotechnology and Bioengineering, Kangwon National UniversityMGENSolutions Biotechnology Research InstituteMGENSolutions Biotechnology Research InstituteDepartment of Biotechnology and Bioengineering, Kangwon National UniversityDepartment of Biotechnology and Bioengineering, Kangwon National UniversityMGENSolutions Biotechnology Research InstituteDepartment of Biotechnology and Bioengineering, Kangwon National UniversityAbstract There is growing evidence that neonatal porcine islet-like cell clusters (NPCCs) isolated from piglets can be used to treat type 1 diabetes in humans. However, graft rejection is a common complication in humans owing to the prevalence of xenoantigens in porcine. Therefore, researchers have investigated various islet encapsulation techniques that could protect against these antigens. To this end, this study presents a robust nano-encapsulation method based on bifunctional polymersomes (PSomes), in which N-hydroxysuccinimide (NHS) and maleimide (Mal) groups conjugated to the PSomes terminal interact with the amine and thiol groups on the surface of NPCCs to induce dual targeting via two covalent bonds. The findings indicate that the ratio of NHS to Mal on PSomes is optimal for dual targeting. Moreover, triiodothyronine (T3) is known to promotes pancreatic islet maturation and differentiation of endocrine cells into beta cells. T3 encapsulated in PSomes is shown to increase the glucose sensitivity of NPCCs and enhance insulin secretion from NPCCs. Furthermore, improvements in the nano-encapsulation efficiency and insulin-secreting capability of NPCCs through dual targeting via dual-Psomes are demonstrated. In conclusion, the proposed nano-encapsulation technique could pave the way for significant advances in islet nano-encapsulation and the imprevement of NPCC immaturity via T3 release.https://doi.org/10.1186/s11671-024-03964-3Drug carrierDual targetingNano-encapsulationNeonatal porcine islet-like cell clusters (NPCCs)Polymersomes |
| spellingShingle | Sang Hoon Lee Minse Kim Eun-Jin Lee Sun Mi Ahn Yu-Rim Ahn Jaewon Choi Jung-Taek Kang Hyun-Ouk Kim Dual-targeted nano-encapsulation of neonatal porcine islet-like cell clusters with triiodothyronine-loaded bifunctional polymersomes Discover Nano Drug carrier Dual targeting Nano-encapsulation Neonatal porcine islet-like cell clusters (NPCCs) Polymersomes |
| title | Dual-targeted nano-encapsulation of neonatal porcine islet-like cell clusters with triiodothyronine-loaded bifunctional polymersomes |
| title_full | Dual-targeted nano-encapsulation of neonatal porcine islet-like cell clusters with triiodothyronine-loaded bifunctional polymersomes |
| title_fullStr | Dual-targeted nano-encapsulation of neonatal porcine islet-like cell clusters with triiodothyronine-loaded bifunctional polymersomes |
| title_full_unstemmed | Dual-targeted nano-encapsulation of neonatal porcine islet-like cell clusters with triiodothyronine-loaded bifunctional polymersomes |
| title_short | Dual-targeted nano-encapsulation of neonatal porcine islet-like cell clusters with triiodothyronine-loaded bifunctional polymersomes |
| title_sort | dual targeted nano encapsulation of neonatal porcine islet like cell clusters with triiodothyronine loaded bifunctional polymersomes |
| topic | Drug carrier Dual targeting Nano-encapsulation Neonatal porcine islet-like cell clusters (NPCCs) Polymersomes |
| url | https://doi.org/10.1186/s11671-024-03964-3 |
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