Protein factors of capacitation and decapacitation in canine seminal plasma
The aim of the present study was to determine the proteins in canine seminal plasma with direct effect on specific sperm parameters during fertilisation. The influence of seminal plasma proteins was fo-cused on three processes: capacitation, hyperactivation and decapacitation. Size exclusion chroma-...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria
2024-03-01
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| Series: | Bulgarian Journal of Veterinary Medicine |
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| _version_ | 1797351524051976192 |
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| author | Ts. Tsvetkov D. Daskalova |
| author_facet | Ts. Tsvetkov D. Daskalova |
| author_sort | Ts. Tsvetkov |
| collection | DOAJ |
| description | The aim of the present study was to determine the proteins in canine seminal plasma with direct effect on specific sperm parameters during fertilisation. The influence of seminal plasma proteins was fo-cused on three processes: capacitation, hyperactivation and decapacitation. Size exclusion chroma-tographic fractional separation of proteins from seminal plasma based on their molecular weights was performed. In addition, two-dimensional electrophoresis of two seminal protein fractions was done. The effect of protein molecules on the motion and kinetic parameters of the male gametes in in vitro conditions was monitored by computer-assisted sperm analysis. The activity of alkaline phosphatase, creatine kinase and alanine aminotransferase in whole seminal plasma and each chromatography fraction was measured spectrophotometrically using the BA-88A semi-automatic chemistry analyzer. The protein fractions with different molecular weights affected the in vitro motility and kinetic characteristics (curvilinear velocity, straightness, linearity and amplitude of lateral head displacement) of spermatozoa, leading to hyperactivation or initiating decapacitation kinetics changes. The detailed study of the seminal plasma proteome would add fundamental information about the processes associated with physiological changes occurring in spermatozoa before fertilisation. The study of the canine seminal plasma proteome could add relevant information about its effects on the fertilising ability of the male gametes and the changes occurring in them before fertilisation. |
| first_indexed | 2024-03-08T13:00:46Z |
| format | Article |
| id | doaj.art-e1002d10dd1c4579837ea91027dfae43 |
| institution | Directory Open Access Journal |
| issn | 1311-1477 1313-3543 |
| language | English |
| last_indexed | 2024-03-08T13:00:46Z |
| publishDate | 2024-03-01 |
| publisher | Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria |
| record_format | Article |
| series | Bulgarian Journal of Veterinary Medicine |
| spelling | doaj.art-e1002d10dd1c4579837ea91027dfae432024-01-19T07:46:47ZengFaculty of Veterinary Medicine, Trakia University, Stara Zagora, BulgariaBulgarian Journal of Veterinary Medicine1311-14771313-35432024-03-01271152610.15547/bjvm.2021-0130Protein factors of capacitation and decapacitation in canine seminal plasmaTs. Tsvetkov0D. Daskalova1Institute of Biology and Immunology of Reproduction “Acad. Kiril Bratanov”, Bulgarian Academy of Sciences, Sofia, BulgariaInstitute of Biology and Immunology of Reproduction “Acad. Kiril Bratanov”, Bulgarian Academy of Sciences, Sofia, BulgariaThe aim of the present study was to determine the proteins in canine seminal plasma with direct effect on specific sperm parameters during fertilisation. The influence of seminal plasma proteins was fo-cused on three processes: capacitation, hyperactivation and decapacitation. Size exclusion chroma-tographic fractional separation of proteins from seminal plasma based on their molecular weights was performed. In addition, two-dimensional electrophoresis of two seminal protein fractions was done. The effect of protein molecules on the motion and kinetic parameters of the male gametes in in vitro conditions was monitored by computer-assisted sperm analysis. The activity of alkaline phosphatase, creatine kinase and alanine aminotransferase in whole seminal plasma and each chromatography fraction was measured spectrophotometrically using the BA-88A semi-automatic chemistry analyzer. The protein fractions with different molecular weights affected the in vitro motility and kinetic characteristics (curvilinear velocity, straightness, linearity and amplitude of lateral head displacement) of spermatozoa, leading to hyperactivation or initiating decapacitation kinetics changes. The detailed study of the seminal plasma proteome would add fundamental information about the processes associated with physiological changes occurring in spermatozoa before fertilisation. The study of the canine seminal plasma proteome could add relevant information about its effects on the fertilising ability of the male gametes and the changes occurring in them before fertilisation.chromatographyproteinsspermatozoatwo-dimensional electrophoresis |
| spellingShingle | Ts. Tsvetkov D. Daskalova Protein factors of capacitation and decapacitation in canine seminal plasma Bulgarian Journal of Veterinary Medicine chromatography proteins spermatozoa two-dimensional electrophoresis |
| title | Protein factors of capacitation and decapacitation in canine seminal plasma |
| title_full | Protein factors of capacitation and decapacitation in canine seminal plasma |
| title_fullStr | Protein factors of capacitation and decapacitation in canine seminal plasma |
| title_full_unstemmed | Protein factors of capacitation and decapacitation in canine seminal plasma |
| title_short | Protein factors of capacitation and decapacitation in canine seminal plasma |
| title_sort | protein factors of capacitation and decapacitation in canine seminal plasma |
| topic | chromatography proteins spermatozoa two-dimensional electrophoresis |
| work_keys_str_mv | AT tstsvetkov proteinfactorsofcapacitationanddecapacitationincanineseminalplasma AT ddaskalova proteinfactorsofcapacitationanddecapacitationincanineseminalplasma |