Surface charge controlled nucleoli selective staining with nanoscale carbon dots.
Organelle selective imaging can reveal structural and functional characters of cells undergoing external stimuli, and is considered critical in revealing biological fundamentals, designing targeted delivery system, and screening potential drugs and therapeutics. This paper describes the nucleoli tar...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0216230 |
| _version_ | 1818726941001252864 |
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| author | Zhijun Zhu Qingxuan Li Ping Li Xiaojie Xun Liyuan Zheng Dandan Ning Ming Su |
| author_facet | Zhijun Zhu Qingxuan Li Ping Li Xiaojie Xun Liyuan Zheng Dandan Ning Ming Su |
| author_sort | Zhijun Zhu |
| collection | DOAJ |
| description | Organelle selective imaging can reveal structural and functional characters of cells undergoing external stimuli, and is considered critical in revealing biological fundamentals, designing targeted delivery system, and screening potential drugs and therapeutics. This paper describes the nucleoli targeting ability of nanoscale carbon dots (including nanodiamond) that are hydrothermally made with controlled surface charges. The surface charges of carbon dots are controlled in the range of -17.9 to -2.84 mV by changing the molar ratio of two precursors, citric acid (CA) and ethylenediamine (EDA). All carbon dots samples show strong fluorescence under wide excitation wavelength, and samples with both negative and positve charges show strong fluorescent contrast from stained nucleoli. The nucleoli selective imaging of live cell has been confirmed with Hoechst staining and nucleoli specific staining (SYTO RNA-select green), and is explained as surface charge heterogeneity on carbon dots. Carbon dots with both negative and positive charges have better ability to penetrate cell and nucleus membranes, and the charge heterogeneity helps carbon dots to bind preferentially to nucleoli, where the electrostatic environment is favored. |
| first_indexed | 2024-12-17T22:06:12Z |
| format | Article |
| id | doaj.art-e102f7337cc141b5864e8c9ef4101a28 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T22:06:12Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e102f7337cc141b5864e8c9ef4101a282022-12-21T21:30:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01145e021623010.1371/journal.pone.0216230Surface charge controlled nucleoli selective staining with nanoscale carbon dots.Zhijun ZhuQingxuan LiPing LiXiaojie XunLiyuan ZhengDandan NingMing SuOrganelle selective imaging can reveal structural and functional characters of cells undergoing external stimuli, and is considered critical in revealing biological fundamentals, designing targeted delivery system, and screening potential drugs and therapeutics. This paper describes the nucleoli targeting ability of nanoscale carbon dots (including nanodiamond) that are hydrothermally made with controlled surface charges. The surface charges of carbon dots are controlled in the range of -17.9 to -2.84 mV by changing the molar ratio of two precursors, citric acid (CA) and ethylenediamine (EDA). All carbon dots samples show strong fluorescence under wide excitation wavelength, and samples with both negative and positve charges show strong fluorescent contrast from stained nucleoli. The nucleoli selective imaging of live cell has been confirmed with Hoechst staining and nucleoli specific staining (SYTO RNA-select green), and is explained as surface charge heterogeneity on carbon dots. Carbon dots with both negative and positive charges have better ability to penetrate cell and nucleus membranes, and the charge heterogeneity helps carbon dots to bind preferentially to nucleoli, where the electrostatic environment is favored.https://doi.org/10.1371/journal.pone.0216230 |
| spellingShingle | Zhijun Zhu Qingxuan Li Ping Li Xiaojie Xun Liyuan Zheng Dandan Ning Ming Su Surface charge controlled nucleoli selective staining with nanoscale carbon dots. PLoS ONE |
| title | Surface charge controlled nucleoli selective staining with nanoscale carbon dots. |
| title_full | Surface charge controlled nucleoli selective staining with nanoscale carbon dots. |
| title_fullStr | Surface charge controlled nucleoli selective staining with nanoscale carbon dots. |
| title_full_unstemmed | Surface charge controlled nucleoli selective staining with nanoscale carbon dots. |
| title_short | Surface charge controlled nucleoli selective staining with nanoscale carbon dots. |
| title_sort | surface charge controlled nucleoli selective staining with nanoscale carbon dots |
| url | https://doi.org/10.1371/journal.pone.0216230 |
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