A novel tissue microarray instrumentation:The HT-1 tissue microarrayer
Background: Tissue microarray (TMA) is a novel and useful tool to efficiently analyze gene expression in histological tissues. Aim: Cost-efficient and easy to use automated tissue arrayers will provide a better instrumentation to generate TMAs. Thus, we designed and produced our tissue microarrayer...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2012-01-01
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| Series: | Indian Journal of Pathology and Microbiology |
| Subjects: | |
| Online Access: | http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2012;volume=55;issue=3;spage=314;epage=318;aulast=Yang |
| _version_ | 1818502756862787584 |
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| author | Jun Yang Mingjuan Zhang Baoshan Su XiaoLi Chen AnJing Kang |
| author_facet | Jun Yang Mingjuan Zhang Baoshan Su XiaoLi Chen AnJing Kang |
| author_sort | Jun Yang |
| collection | DOAJ |
| description | Background: Tissue microarray (TMA) is a novel and useful tool to efficiently analyze gene expression in histological tissues. Aim: Cost-efficient and easy to use automated tissue arrayers will provide a better instrumentation to generate TMAs. Thus, we designed and produced our tissue microarrayer to meet these needs. Materials and Methods: The HT-1 tissue microarrayer we designed and manufactured consists primarily of four parts, including an instrument to make array pores for the recipient paraffin blocks, a punch needle, an instrument for negative-pressure embedding, and a special manipulator. By using the HT-1, 14 different TMAs were made to accommodate 312 cases of tissues and TMA sections were tested by hematoxylin-eosin (H&E) staining, in situ hybridization, and immunohistochemistry. Results: Expand: Hematoxylin and eosin staining showed that the tissue cylinders were similar, even, and in order on the slides. Most importantly, the HT-1 microarrayer can make array pores in the recipient paraffin block with a single application in seconds. The HT-1 also contains a unique negative pressure system for embedding TMA blocks. In addition, HT-1 can make tissue cylinders with the same levels and depth for equally embedded and sectioning. Conclusions: The HT-1 tissue microarrayer is a device that is simple, economical and easy to use. |
| first_indexed | 2024-12-10T21:14:42Z |
| format | Article |
| id | doaj.art-e1041a4b13b3437d8c3f7bf07d2d9c5d |
| institution | Directory Open Access Journal |
| issn | 0377-4929 |
| language | English |
| last_indexed | 2024-12-10T21:14:42Z |
| publishDate | 2012-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Pathology and Microbiology |
| spelling | doaj.art-e1041a4b13b3437d8c3f7bf07d2d9c5d2022-12-22T01:33:21ZengWolters Kluwer Medknow PublicationsIndian Journal of Pathology and Microbiology0377-49292012-01-0155331431810.4103/0377-4929.101736A novel tissue microarray instrumentation:The HT-1 tissue microarrayerJun YangMingjuan ZhangBaoshan SuXiaoLi ChenAnJing KangBackground: Tissue microarray (TMA) is a novel and useful tool to efficiently analyze gene expression in histological tissues. Aim: Cost-efficient and easy to use automated tissue arrayers will provide a better instrumentation to generate TMAs. Thus, we designed and produced our tissue microarrayer to meet these needs. Materials and Methods: The HT-1 tissue microarrayer we designed and manufactured consists primarily of four parts, including an instrument to make array pores for the recipient paraffin blocks, a punch needle, an instrument for negative-pressure embedding, and a special manipulator. By using the HT-1, 14 different TMAs were made to accommodate 312 cases of tissues and TMA sections were tested by hematoxylin-eosin (H&E) staining, in situ hybridization, and immunohistochemistry. Results: Expand: Hematoxylin and eosin staining showed that the tissue cylinders were similar, even, and in order on the slides. Most importantly, the HT-1 microarrayer can make array pores in the recipient paraffin block with a single application in seconds. The HT-1 also contains a unique negative pressure system for embedding TMA blocks. In addition, HT-1 can make tissue cylinders with the same levels and depth for equally embedded and sectioning. Conclusions: The HT-1 tissue microarrayer is a device that is simple, economical and easy to use.http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2012;volume=55;issue=3;spage=314;epage=318;aulast=YangTissue microarrayertissue microarraystissue microarray blocks |
| spellingShingle | Jun Yang Mingjuan Zhang Baoshan Su XiaoLi Chen AnJing Kang A novel tissue microarray instrumentation:The HT-1 tissue microarrayer Indian Journal of Pathology and Microbiology Tissue microarrayer tissue microarrays tissue microarray blocks |
| title | A novel tissue microarray instrumentation:The HT-1 tissue microarrayer |
| title_full | A novel tissue microarray instrumentation:The HT-1 tissue microarrayer |
| title_fullStr | A novel tissue microarray instrumentation:The HT-1 tissue microarrayer |
| title_full_unstemmed | A novel tissue microarray instrumentation:The HT-1 tissue microarrayer |
| title_short | A novel tissue microarray instrumentation:The HT-1 tissue microarrayer |
| title_sort | novel tissue microarray instrumentation the ht 1 tissue microarrayer |
| topic | Tissue microarrayer tissue microarrays tissue microarray blocks |
| url | http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2012;volume=55;issue=3;spage=314;epage=318;aulast=Yang |
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