MicroRNA-140-5p Inhibits the Progression of Colorectal Cancer by Targeting VEGFA
Background: microRNAs (miRNAs) are small non-coding RNAs and have been shown to play a crucial role in the colorectal cancer (CRC) tumorigenesis and progression. The aim of this study was to investigate the clinical significance and prognostic value of miR-140-5p in CRC. The exact functions and the...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2015-09-01
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| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/430237 |
| _version_ | 1819145617399611392 |
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| author | Wenbo Zhang Chen Zou Lei Pan Ying Xu Weidong Qi Gui Ma Yongzhong Hou Pengcheng Jiang |
| author_facet | Wenbo Zhang Chen Zou Lei Pan Ying Xu Weidong Qi Gui Ma Yongzhong Hou Pengcheng Jiang |
| author_sort | Wenbo Zhang |
| collection | DOAJ |
| description | Background: microRNAs (miRNAs) are small non-coding RNAs and have been shown to play a crucial role in the colorectal cancer (CRC) tumorigenesis and progression. The aim of this study was to investigate the clinical significance and prognostic value of miR-140-5p in CRC. The exact functions and the underlying molecular mechanisms of miR-140-5p in CRC was further determined. Methods: miR-140-5p expression was detected in CRC samples, their adjacent nontumor tissues as well as CRC cell lines by RT-qPCR. Cell proliferation was detected using CCK-8, and cell invasion and migration were evaluated using Transwell assay. The direct regulation of VEGFA by miR-140-5p was identified using luciferase reporter assay. Results: miR-140-5p was significantly dowregulated in CRC tissues and cell lines. Downregulation of miR-140-5p was significantly correlated with advanced CRC stage and poorer overall survival. Both gain-of-function and loss of function studies demonstrated that miR-140-5p acted as a tumor suppressor by inhibiting cell proliferation, migration and invasion. Integrated analysis identified VEGFA as a direct and functional target gene of miR-140-5p. Silencing VEGFA by small interfering RNA (siRNA) resembled the phenotype resulting from ectopic miR-140-5p expression, while overexpression of VEGFA attenuated the effect of miR-140-5p on CRC cells. Conclusions: Our results suggested a tumor suppressive role of miR-140-5p in CRC tumorigenesis and progression by targeting VEGFA. |
| first_indexed | 2024-12-22T13:00:53Z |
| format | Article |
| id | doaj.art-e108d099d6164d93a547f74c609b50ce |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-22T13:00:53Z |
| publishDate | 2015-09-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-e108d099d6164d93a547f74c609b50ce2022-12-21T18:25:00ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782015-09-013731123113310.1159/000430237430237MicroRNA-140-5p Inhibits the Progression of Colorectal Cancer by Targeting VEGFAWenbo ZhangChen ZouLei PanYing XuWeidong QiGui MaYongzhong HouPengcheng JiangBackground: microRNAs (miRNAs) are small non-coding RNAs and have been shown to play a crucial role in the colorectal cancer (CRC) tumorigenesis and progression. The aim of this study was to investigate the clinical significance and prognostic value of miR-140-5p in CRC. The exact functions and the underlying molecular mechanisms of miR-140-5p in CRC was further determined. Methods: miR-140-5p expression was detected in CRC samples, their adjacent nontumor tissues as well as CRC cell lines by RT-qPCR. Cell proliferation was detected using CCK-8, and cell invasion and migration were evaluated using Transwell assay. The direct regulation of VEGFA by miR-140-5p was identified using luciferase reporter assay. Results: miR-140-5p was significantly dowregulated in CRC tissues and cell lines. Downregulation of miR-140-5p was significantly correlated with advanced CRC stage and poorer overall survival. Both gain-of-function and loss of function studies demonstrated that miR-140-5p acted as a tumor suppressor by inhibiting cell proliferation, migration and invasion. Integrated analysis identified VEGFA as a direct and functional target gene of miR-140-5p. Silencing VEGFA by small interfering RNA (siRNA) resembled the phenotype resulting from ectopic miR-140-5p expression, while overexpression of VEGFA attenuated the effect of miR-140-5p on CRC cells. Conclusions: Our results suggested a tumor suppressive role of miR-140-5p in CRC tumorigenesis and progression by targeting VEGFA.http://www.karger.com/Article/FullText/430237Colorectal cancerMiR-140-5pVEGFA |
| spellingShingle | Wenbo Zhang Chen Zou Lei Pan Ying Xu Weidong Qi Gui Ma Yongzhong Hou Pengcheng Jiang MicroRNA-140-5p Inhibits the Progression of Colorectal Cancer by Targeting VEGFA Cellular Physiology and Biochemistry Colorectal cancer MiR-140-5p VEGFA |
| title | MicroRNA-140-5p Inhibits the Progression of Colorectal Cancer by Targeting VEGFA |
| title_full | MicroRNA-140-5p Inhibits the Progression of Colorectal Cancer by Targeting VEGFA |
| title_fullStr | MicroRNA-140-5p Inhibits the Progression of Colorectal Cancer by Targeting VEGFA |
| title_full_unstemmed | MicroRNA-140-5p Inhibits the Progression of Colorectal Cancer by Targeting VEGFA |
| title_short | MicroRNA-140-5p Inhibits the Progression of Colorectal Cancer by Targeting VEGFA |
| title_sort | microrna 140 5p inhibits the progression of colorectal cancer by targeting vegfa |
| topic | Colorectal cancer MiR-140-5p VEGFA |
| url | http://www.karger.com/Article/FullText/430237 |
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