Waste not, want not: Microsatellites remain an economical and informative technology for conservation genetics
Abstract Comparisons of microsatellites and single‐nucleotide polymorphisms (SNPs) have found that SNPs outperform microsatellites in population genetic analyses, questioning the continued utility of microsatellites in population and landscape genetics. Yet, highly polymorphic markers may be of valu...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-11-01
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| Series: | Ecology and Evolution |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ece3.8250 |
| _version_ | 1819004724377026560 |
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| author | Samantha S. Hauser Giridhar Athrey Paul L. Leberg |
| author_facet | Samantha S. Hauser Giridhar Athrey Paul L. Leberg |
| author_sort | Samantha S. Hauser |
| collection | DOAJ |
| description | Abstract Comparisons of microsatellites and single‐nucleotide polymorphisms (SNPs) have found that SNPs outperform microsatellites in population genetic analyses, questioning the continued utility of microsatellites in population and landscape genetics. Yet, highly polymorphic markers may be of value in species that have reduced genetic variation. This study repeated previous analyses that used microsatellites with SNPs developed from ddRAD sequencing in the black‐capped vireo source‐sink system. SNPs provided greater resolution of genetic diversity, population differentiation, and migrant detection but could not reconstruct parentage relationships due to insufficient heterozygosities. The biological inferences made by both sets of markers were similar: asymmetrical gene flow from source sites to the remaining sink sites. With the landscape genetic analyses, we found different results between the two molecular markers, but associations of the top environmental features (riparian, open habitat, agriculture, and human development) with dispersal estimates were shared between marker types. Despite the higher precision of SNPs, we find that microsatellites effectively uncover population processes and patterns and are superior for parentage analyses in this species with reduced genetic diversity. This study illustrates the continued applicability and relevance of microsatellites in population genetic research. |
| first_indexed | 2024-12-20T23:41:27Z |
| format | Article |
| id | doaj.art-e109e3dec2474415a623239063c0ac90 |
| institution | Directory Open Access Journal |
| issn | 2045-7758 |
| language | English |
| last_indexed | 2024-12-20T23:41:27Z |
| publishDate | 2021-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj.art-e109e3dec2474415a623239063c0ac902022-12-21T19:23:04ZengWileyEcology and Evolution2045-77582021-11-011122158001581410.1002/ece3.8250Waste not, want not: Microsatellites remain an economical and informative technology for conservation geneticsSamantha S. Hauser0Giridhar Athrey1Paul L. Leberg2Department of Biology University of Louisiana at Lafayette Lafayette Louisiana USAFaculty of Ecology and Evolutionary Biology Texas A&M University College Station Texas USADepartment of Biology University of Louisiana at Lafayette Lafayette Louisiana USAAbstract Comparisons of microsatellites and single‐nucleotide polymorphisms (SNPs) have found that SNPs outperform microsatellites in population genetic analyses, questioning the continued utility of microsatellites in population and landscape genetics. Yet, highly polymorphic markers may be of value in species that have reduced genetic variation. This study repeated previous analyses that used microsatellites with SNPs developed from ddRAD sequencing in the black‐capped vireo source‐sink system. SNPs provided greater resolution of genetic diversity, population differentiation, and migrant detection but could not reconstruct parentage relationships due to insufficient heterozygosities. The biological inferences made by both sets of markers were similar: asymmetrical gene flow from source sites to the remaining sink sites. With the landscape genetic analyses, we found different results between the two molecular markers, but associations of the top environmental features (riparian, open habitat, agriculture, and human development) with dispersal estimates were shared between marker types. Despite the higher precision of SNPs, we find that microsatellites effectively uncover population processes and patterns and are superior for parentage analyses in this species with reduced genetic diversity. This study illustrates the continued applicability and relevance of microsatellites in population genetic research.https://doi.org/10.1002/ece3.8250genomicsmolecular markersnext‐generation sequencingparentagepopulation structureresistance surfaces |
| spellingShingle | Samantha S. Hauser Giridhar Athrey Paul L. Leberg Waste not, want not: Microsatellites remain an economical and informative technology for conservation genetics Ecology and Evolution genomics molecular markers next‐generation sequencing parentage population structure resistance surfaces |
| title | Waste not, want not: Microsatellites remain an economical and informative technology for conservation genetics |
| title_full | Waste not, want not: Microsatellites remain an economical and informative technology for conservation genetics |
| title_fullStr | Waste not, want not: Microsatellites remain an economical and informative technology for conservation genetics |
| title_full_unstemmed | Waste not, want not: Microsatellites remain an economical and informative technology for conservation genetics |
| title_short | Waste not, want not: Microsatellites remain an economical and informative technology for conservation genetics |
| title_sort | waste not want not microsatellites remain an economical and informative technology for conservation genetics |
| topic | genomics molecular markers next‐generation sequencing parentage population structure resistance surfaces |
| url | https://doi.org/10.1002/ece3.8250 |
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