Dietary Nitrate Protects Against Skin Flap Ischemia-Reperfusion Injury in Rats via Modulation of Antioxidative Action and Reduction of Inflammatory Responses
Dietary nitrate, found abundant in green vegetables, can be absorbed into the blood and be converted to nitric oxide (NO) in the body. Dietary nitrate has been proved to have many positive physiological functions in the body. Here, we evaluated the therapeutic effects of dietary nitrate on skin flap...
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| Format: | Article |
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Frontiers Media S.A.
2020-01-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2019.01605/full |
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| author | Hao Cui Hao Cui Yuanyong Feng Yuanyong Feng Chuanliang Shu Rongtao Yuan Lingxue Bu Lingxue Bu Muyun Jia Muyun Jia Baoxing Pang Baoxing Pang |
| author_facet | Hao Cui Hao Cui Yuanyong Feng Yuanyong Feng Chuanliang Shu Rongtao Yuan Lingxue Bu Lingxue Bu Muyun Jia Muyun Jia Baoxing Pang Baoxing Pang |
| author_sort | Hao Cui |
| collection | DOAJ |
| description | Dietary nitrate, found abundant in green vegetables, can be absorbed into the blood and be converted to nitric oxide (NO) in the body. Dietary nitrate has been proved to have many positive physiological functions in the body. Here, we evaluated the therapeutic effects of dietary nitrate on skin flap recovery following ischemia reperfusion (IR). Wistar rats were pretreated with nitrate from one week prior to ischemia to the end of reperfusion. It was found that oral administration of nitrate increased serum nitrate and nitrite levels, protected cells from apoptosis, and attenuated flap tissue edema. In the meantime, the oxidative stress marker malondialdehyde was reduced, while the activities of antioxidant enzymes were restored after nitrate treatment. Moreover, the macrophage and neutrophil infiltration in the flap was significantly attenuated by nitrate supplementation, as were the pro-inflammatory cytokines. In sum, we found that oral administration of nitrate can attenuate skin flap IR injury through the regulation of oxidative stress and inflammatory response. |
| first_indexed | 2024-04-13T03:10:16Z |
| format | Article |
| id | doaj.art-e10a30fd39f541d4a50912a61c0e7027 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-13T03:10:16Z |
| publishDate | 2020-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-e10a30fd39f541d4a50912a61c0e70272022-12-22T03:05:05ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122020-01-011010.3389/fphar.2019.01605500077Dietary Nitrate Protects Against Skin Flap Ischemia-Reperfusion Injury in Rats via Modulation of Antioxidative Action and Reduction of Inflammatory ResponsesHao Cui0Hao Cui1Yuanyong Feng2Yuanyong Feng3Chuanliang Shu4Rongtao Yuan5Lingxue Bu6Lingxue Bu7Muyun Jia8Muyun Jia9Baoxing Pang10Baoxing Pang11Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, ChinaSchool of Stomatology of Qingdao University, Qingdao, ChinaDepartment of Oral and Maxillofacial Surgery, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, ChinaSchool of Stomatology of Qingdao University, Qingdao, ChinaDepartment of Stomatology, The Affiliated Qingdao Hiser Hospital of Qingdao University, Qingdao, ChinaQingdao Municipal Hospital, Affiliated to Shandong University, Qingdao, ChinaDepartment of Oral and Maxillofacial Surgery, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, ChinaSchool of Stomatology of Qingdao University, Qingdao, ChinaDepartment of Oral and Maxillofacial Surgery, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, ChinaSchool of Stomatology of Qingdao University, Qingdao, ChinaDepartment of Oral and Maxillofacial Surgery, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, ChinaSchool of Stomatology of Qingdao University, Qingdao, ChinaDietary nitrate, found abundant in green vegetables, can be absorbed into the blood and be converted to nitric oxide (NO) in the body. Dietary nitrate has been proved to have many positive physiological functions in the body. Here, we evaluated the therapeutic effects of dietary nitrate on skin flap recovery following ischemia reperfusion (IR). Wistar rats were pretreated with nitrate from one week prior to ischemia to the end of reperfusion. It was found that oral administration of nitrate increased serum nitrate and nitrite levels, protected cells from apoptosis, and attenuated flap tissue edema. In the meantime, the oxidative stress marker malondialdehyde was reduced, while the activities of antioxidant enzymes were restored after nitrate treatment. Moreover, the macrophage and neutrophil infiltration in the flap was significantly attenuated by nitrate supplementation, as were the pro-inflammatory cytokines. In sum, we found that oral administration of nitrate can attenuate skin flap IR injury through the regulation of oxidative stress and inflammatory response.https://www.frontiersin.org/article/10.3389/fphar.2019.01605/fullnitrateischemia-reperfusion injurynitric oxideoxidative stressinflammationapoptosis |
| spellingShingle | Hao Cui Hao Cui Yuanyong Feng Yuanyong Feng Chuanliang Shu Rongtao Yuan Lingxue Bu Lingxue Bu Muyun Jia Muyun Jia Baoxing Pang Baoxing Pang Dietary Nitrate Protects Against Skin Flap Ischemia-Reperfusion Injury in Rats via Modulation of Antioxidative Action and Reduction of Inflammatory Responses Frontiers in Pharmacology nitrate ischemia-reperfusion injury nitric oxide oxidative stress inflammation apoptosis |
| title | Dietary Nitrate Protects Against Skin Flap Ischemia-Reperfusion Injury in Rats via Modulation of Antioxidative Action and Reduction of Inflammatory Responses |
| title_full | Dietary Nitrate Protects Against Skin Flap Ischemia-Reperfusion Injury in Rats via Modulation of Antioxidative Action and Reduction of Inflammatory Responses |
| title_fullStr | Dietary Nitrate Protects Against Skin Flap Ischemia-Reperfusion Injury in Rats via Modulation of Antioxidative Action and Reduction of Inflammatory Responses |
| title_full_unstemmed | Dietary Nitrate Protects Against Skin Flap Ischemia-Reperfusion Injury in Rats via Modulation of Antioxidative Action and Reduction of Inflammatory Responses |
| title_short | Dietary Nitrate Protects Against Skin Flap Ischemia-Reperfusion Injury in Rats via Modulation of Antioxidative Action and Reduction of Inflammatory Responses |
| title_sort | dietary nitrate protects against skin flap ischemia reperfusion injury in rats via modulation of antioxidative action and reduction of inflammatory responses |
| topic | nitrate ischemia-reperfusion injury nitric oxide oxidative stress inflammation apoptosis |
| url | https://www.frontiersin.org/article/10.3389/fphar.2019.01605/full |
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