Relationship of lipoprotein-associated phospholipase A2 and oxidized low density lipoprotein in carotid atherosclerosis[S]
Plasma levels of lipoprotein-associated phospholipase A2 (Lp-PLA2) and oxidized low density lipoprotein (oxLDL) have been identified as risk factors for cardiovascular disease. Lp-PLA2 is the sole enzyme responsible for the hydrolysis of oxidized phospholipids on LDL particles in atherosclerotic pla...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2009-09-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520307264 |
| _version_ | 1818732464587145216 |
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| author | Kasey C. Vickers Colin T. Maguire Robert Wolfert Alan R. Burns Michael Reardon Richard Geis Paul Holvoet Joel D. Morrisett |
| author_facet | Kasey C. Vickers Colin T. Maguire Robert Wolfert Alan R. Burns Michael Reardon Richard Geis Paul Holvoet Joel D. Morrisett |
| author_sort | Kasey C. Vickers |
| collection | DOAJ |
| description | Plasma levels of lipoprotein-associated phospholipase A2 (Lp-PLA2) and oxidized low density lipoprotein (oxLDL) have been identified as risk factors for cardiovascular disease. Lp-PLA2 is the sole enzyme responsible for the hydrolysis of oxidized phospholipids on LDL particles in atherosclerotic plaques. We have studied the relationship between Lp-PLA2 and oxLDL in carotid endarterectomy (CEA) tissues and in matched plasmas. In extracts from CEA anatomical segments, the levels of oxLDL were significantly associated with the levels of Lp-PLA2 protein (r = 0.497) and activity (r = 0.615). OxLDL and Lp-PLA2 mass/activity were most abundant in the carotid bifurcation and internal segments where plaque was most abundant. In extracts from CEA atheroma, the levels of oxLDL and Lp-PLA2 were significantly correlated (r = 0.634). In matched plasma and atheroma extracts, the levels of Lp-PLA2 were negatively correlated (r = − 0.578). The ratio of Lp-PLA2 to oxLDL was higher in atheromatous tissue (277:1) than in normal tissue (135:1) and plasma (13:1). Immunohistochemical experiments indicated that in plaques, oxLDL and Lp-PLA2 existed in overlapping but distinctly different distribution. Fluorescence microscopy showed both oxLDL and Lp-PLA2 epitopes on the same LDL particle in plasma but not in plaque. These results suggest that the relationship between Lp-PLA2 and oxLDL in the atherosclerotic plaque is different from that in the plasma compartment. |
| first_indexed | 2024-12-17T23:33:59Z |
| format | Article |
| id | doaj.art-e10dd9be43fd4eb3935e724bc0d838a2 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T23:33:59Z |
| publishDate | 2009-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-e10dd9be43fd4eb3935e724bc0d838a22022-12-21T21:28:35ZengElsevierJournal of Lipid Research0022-22752009-09-0150917351743Relationship of lipoprotein-associated phospholipase A2 and oxidized low density lipoprotein in carotid atherosclerosis[S]Kasey C. Vickers0Colin T. Maguire1Robert Wolfert2Alan R. Burns3Michael Reardon4Richard Geis5Paul Holvoet6Joel D. Morrisett7Department of Medicine, Baylor College of Medicine, Houston, TXDepartment of Medicine, Baylor College of Medicine, Houston, TXdiaDexus Inc., South San Francisco, CADepartment of Medicine, Baylor College of Medicine, Houston, TXDepartment of Surgery, Methodist Hospital, Houston, TXDepartment of Surgery, Methodist Hospital, Houston, TXDepartment of Cardiovascular Diseases, Katholieke Universiteit, Leuven, BelgiumTo whom correspondence should be addressed.; Department of Medicine, Baylor College of Medicine, Houston, TX; Department of Biochemistry/Molecular Biology, Baylor College of Medicine, Houston, TXPlasma levels of lipoprotein-associated phospholipase A2 (Lp-PLA2) and oxidized low density lipoprotein (oxLDL) have been identified as risk factors for cardiovascular disease. Lp-PLA2 is the sole enzyme responsible for the hydrolysis of oxidized phospholipids on LDL particles in atherosclerotic plaques. We have studied the relationship between Lp-PLA2 and oxLDL in carotid endarterectomy (CEA) tissues and in matched plasmas. In extracts from CEA anatomical segments, the levels of oxLDL were significantly associated with the levels of Lp-PLA2 protein (r = 0.497) and activity (r = 0.615). OxLDL and Lp-PLA2 mass/activity were most abundant in the carotid bifurcation and internal segments where plaque was most abundant. In extracts from CEA atheroma, the levels of oxLDL and Lp-PLA2 were significantly correlated (r = 0.634). In matched plasma and atheroma extracts, the levels of Lp-PLA2 were negatively correlated (r = − 0.578). The ratio of Lp-PLA2 to oxLDL was higher in atheromatous tissue (277:1) than in normal tissue (135:1) and plasma (13:1). Immunohistochemical experiments indicated that in plaques, oxLDL and Lp-PLA2 existed in overlapping but distinctly different distribution. Fluorescence microscopy showed both oxLDL and Lp-PLA2 epitopes on the same LDL particle in plasma but not in plaque. These results suggest that the relationship between Lp-PLA2 and oxLDL in the atherosclerotic plaque is different from that in the plasma compartment.http://www.sciencedirect.com/science/article/pii/S0022227520307264carotid endarterectomyrisk factorsatherosclerotic plaquelysophosphatidylcholine |
| spellingShingle | Kasey C. Vickers Colin T. Maguire Robert Wolfert Alan R. Burns Michael Reardon Richard Geis Paul Holvoet Joel D. Morrisett Relationship of lipoprotein-associated phospholipase A2 and oxidized low density lipoprotein in carotid atherosclerosis[S] Journal of Lipid Research carotid endarterectomy risk factors atherosclerotic plaque lysophosphatidylcholine |
| title | Relationship of lipoprotein-associated phospholipase A2 and oxidized low density lipoprotein in carotid atherosclerosis[S] |
| title_full | Relationship of lipoprotein-associated phospholipase A2 and oxidized low density lipoprotein in carotid atherosclerosis[S] |
| title_fullStr | Relationship of lipoprotein-associated phospholipase A2 and oxidized low density lipoprotein in carotid atherosclerosis[S] |
| title_full_unstemmed | Relationship of lipoprotein-associated phospholipase A2 and oxidized low density lipoprotein in carotid atherosclerosis[S] |
| title_short | Relationship of lipoprotein-associated phospholipase A2 and oxidized low density lipoprotein in carotid atherosclerosis[S] |
| title_sort | relationship of lipoprotein associated phospholipase a2 and oxidized low density lipoprotein in carotid atherosclerosis s |
| topic | carotid endarterectomy risk factors atherosclerotic plaque lysophosphatidylcholine |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520307264 |
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