Molecular Analysis of the Enteric Protozoa Associated with Acute Diarrhea in Hospitalized Children
Pediatric diarrhea is a common cause of death among children under 5 years of age. In the current study, we investigated the frequency of intestinal parasites among 580 pediatric patients with chronic diarrhea. Parasitic protozoa (all species combined) were detected by molecular tools in 22.9% of th...
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Frontiers Media S.A.
2017-08-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fcimb.2017.00343/full |
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| author | Sonia Boughattas Jerzy M. Behnke Khalid Al-Ansari Aarti Sharma Wafa Abu-Alainin Asma Al-Thani Marawan A. Abu-Madi |
| author_facet | Sonia Boughattas Jerzy M. Behnke Khalid Al-Ansari Aarti Sharma Wafa Abu-Alainin Asma Al-Thani Marawan A. Abu-Madi |
| author_sort | Sonia Boughattas |
| collection | DOAJ |
| description | Pediatric diarrhea is a common cause of death among children under 5 years of age. In the current study, we investigated the frequency of intestinal parasites among 580 pediatric patients with chronic diarrhea. Parasitic protozoa (all species combined) were detected by molecular tools in 22.9% of the children and the most common parasite was Cryptosporidium spp. (15.1%). Blastocystis hominis was detected in 4.7%, Dientamoeba fragilis in 4%, Giardia duodenalis in 1.7%, and Entamoeba histolytica in 0.17%. Protozoan infections were observed among all regional groups, but prevalence was highest among Qatari subjects and during the winter season. Typing of Cryptosporidium spp. revealed a predominance of Cryptosporidium parvum in 92% of cases with mostly the IIdA20G1 subtype. Subtypes IIdA19G2, IIdA18G2, IIdA18G1, IIdA17G1, IIdA16G1, and IIdA14G1 were also detected. For Cryptosporidium hominis, IbA10G2 and IbA9G3 subtypes were identified. This study provides supplementary information for implementing prevention and control strategies to reduce the burden of these pediatric protozoan infections. Further analyses are required to better understand the local epidemiology and transmission of Cryptosporidium spp. in Qatar. |
| first_indexed | 2024-04-13T02:21:32Z |
| format | Article |
| id | doaj.art-e1113bfadc8645809033dbacb1621b60 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-13T02:21:32Z |
| publishDate | 2017-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-e1113bfadc8645809033dbacb1621b602022-12-22T03:06:57ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882017-08-01710.3389/fcimb.2017.00343265012Molecular Analysis of the Enteric Protozoa Associated with Acute Diarrhea in Hospitalized ChildrenSonia Boughattas0Jerzy M. Behnke1Khalid Al-Ansari2Aarti Sharma3Wafa Abu-Alainin4Asma Al-Thani5Marawan A. Abu-Madi6Department of Biomedical Science, College of Health Sciences, Biomedical Research Center, Qatar UniversityDoha, QatarSchool of Life Sciences, University of Nottingham, University ParkNottingham, United KingdomHamad Medical Corporation, Paediatric Emergency CenterDoha, QatarDepartment of Biomedical Science, College of Health Sciences, Biomedical Research Center, Qatar UniversityDoha, QatarMolecular Genetics Laboratory, Department of Laboratory Medicine and Pathology, Hamad Medical CorporationDoha, QatarDepartment of Biomedical Science, College of Health Sciences, Biomedical Research Center, Qatar UniversityDoha, QatarDepartment of Biomedical Science, College of Health Sciences, Biomedical Research Center, Qatar UniversityDoha, QatarPediatric diarrhea is a common cause of death among children under 5 years of age. In the current study, we investigated the frequency of intestinal parasites among 580 pediatric patients with chronic diarrhea. Parasitic protozoa (all species combined) were detected by molecular tools in 22.9% of the children and the most common parasite was Cryptosporidium spp. (15.1%). Blastocystis hominis was detected in 4.7%, Dientamoeba fragilis in 4%, Giardia duodenalis in 1.7%, and Entamoeba histolytica in 0.17%. Protozoan infections were observed among all regional groups, but prevalence was highest among Qatari subjects and during the winter season. Typing of Cryptosporidium spp. revealed a predominance of Cryptosporidium parvum in 92% of cases with mostly the IIdA20G1 subtype. Subtypes IIdA19G2, IIdA18G2, IIdA18G1, IIdA17G1, IIdA16G1, and IIdA14G1 were also detected. For Cryptosporidium hominis, IbA10G2 and IbA9G3 subtypes were identified. This study provides supplementary information for implementing prevention and control strategies to reduce the burden of these pediatric protozoan infections. Further analyses are required to better understand the local epidemiology and transmission of Cryptosporidium spp. in Qatar.http://journal.frontiersin.org/article/10.3389/fcimb.2017.00343/fullprotozoadiarrheapediatricsQatarRT-PCRstatistical analysis |
| spellingShingle | Sonia Boughattas Jerzy M. Behnke Khalid Al-Ansari Aarti Sharma Wafa Abu-Alainin Asma Al-Thani Marawan A. Abu-Madi Molecular Analysis of the Enteric Protozoa Associated with Acute Diarrhea in Hospitalized Children Frontiers in Cellular and Infection Microbiology protozoa diarrhea pediatrics Qatar RT-PCR statistical analysis |
| title | Molecular Analysis of the Enteric Protozoa Associated with Acute Diarrhea in Hospitalized Children |
| title_full | Molecular Analysis of the Enteric Protozoa Associated with Acute Diarrhea in Hospitalized Children |
| title_fullStr | Molecular Analysis of the Enteric Protozoa Associated with Acute Diarrhea in Hospitalized Children |
| title_full_unstemmed | Molecular Analysis of the Enteric Protozoa Associated with Acute Diarrhea in Hospitalized Children |
| title_short | Molecular Analysis of the Enteric Protozoa Associated with Acute Diarrhea in Hospitalized Children |
| title_sort | molecular analysis of the enteric protozoa associated with acute diarrhea in hospitalized children |
| topic | protozoa diarrhea pediatrics Qatar RT-PCR statistical analysis |
| url | http://journal.frontiersin.org/article/10.3389/fcimb.2017.00343/full |
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