Utility of CD138/syndecan-1 immunohistochemistry for localization of plasmacytes is tissue-dependent in B6 mice
Abstract Objective Inflammation is present in many diseases and identification of immune cell infiltration is a common assessment. CD138 (syndecan-1) is a recommended immunohistochemical marker for human plasmacytes although it is also expressed in various epithelia and tumors. Similarly, CD138 is a...
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| Format: | Article |
| Language: | English |
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BMC
2022-06-01
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| Series: | BMC Research Notes |
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| Online Access: | https://doi.org/10.1186/s13104-022-06100-5 |
| _version_ | 1828315809208860672 |
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| author | David K. Meyerholz Mariah R. Leidinger J. Adam Goeken Thomas R. Businga Allison Akers Sebastian Vizuett Courtney A. Kaemmer Jordan L. Kohlmeyer Rebecca D. Dodd Dawn E. Quelle |
| author_facet | David K. Meyerholz Mariah R. Leidinger J. Adam Goeken Thomas R. Businga Allison Akers Sebastian Vizuett Courtney A. Kaemmer Jordan L. Kohlmeyer Rebecca D. Dodd Dawn E. Quelle |
| author_sort | David K. Meyerholz |
| collection | DOAJ |
| description | Abstract Objective Inflammation is present in many diseases and identification of immune cell infiltration is a common assessment. CD138 (syndecan-1) is a recommended immunohistochemical marker for human plasmacytes although it is also expressed in various epithelia and tumors. Similarly, CD138 is a marker for murine plasmacytes, but its tissue immunostaining is not well-defined. Endogenous CD138 expression is an important confounding factor when evaluating plasmacyte infiltration. We studied two plasmacyte markers (CD138 and Kappa light chains) for endogenous immunostaining in five organs and one tumor from B6 mice. Results Plasmacytes in Peyer’s patches were positive for CD138 and Kappa markers without endogenous immunostaining. Endogenous CD138 immunostaining was widespread in liver, kidney, lung and a malignant peripheral nerve sheath tumor (MPNST) versus regionalized immunostaining in skin and small intestine wall. Endogenous Kappa immunostaining was absent in all tissues except for plasmacytes. Tissues with widespread endogenous CD138 immunostaining were contrasted by absence of endogenous Kappa immunostaining. Here, plasmacytes would not be distinguished by CD138, but would be obvious by Kappa immunostaining. Our study suggests that utility of immunostaining for plasmacytes by CD138 is tissue dependent in mice. Additionally, Kappa immunostaining may be a useful alternative in mouse tissues with confounding endogenous CD138 immunostaining. |
| first_indexed | 2024-04-13T17:05:34Z |
| format | Article |
| id | doaj.art-e118437788d34416b465bf938783c482 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-04-13T17:05:34Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-e118437788d34416b465bf938783c4822022-12-22T02:38:29ZengBMCBMC Research Notes1756-05002022-06-011511710.1186/s13104-022-06100-5Utility of CD138/syndecan-1 immunohistochemistry for localization of plasmacytes is tissue-dependent in B6 miceDavid K. Meyerholz0Mariah R. Leidinger1J. Adam Goeken2Thomas R. Businga3Allison Akers4Sebastian Vizuett5Courtney A. Kaemmer6Jordan L. Kohlmeyer7Rebecca D. Dodd8Dawn E. Quelle9Department of Pathology, University of IowaDepartment of Pathology, University of IowaDepartment of Pathology, University of IowaDepartment of Pathology, University of IowaDepartment of Pathology, University of IowaDepartment of Pathology, University of IowaDepartment of Neuroscience and Pharmacology, University of IowaDepartment of Pathology, University of IowaDepartment of Internal Medicine, University of IowaDepartment of Pathology, University of IowaAbstract Objective Inflammation is present in many diseases and identification of immune cell infiltration is a common assessment. CD138 (syndecan-1) is a recommended immunohistochemical marker for human plasmacytes although it is also expressed in various epithelia and tumors. Similarly, CD138 is a marker for murine plasmacytes, but its tissue immunostaining is not well-defined. Endogenous CD138 expression is an important confounding factor when evaluating plasmacyte infiltration. We studied two plasmacyte markers (CD138 and Kappa light chains) for endogenous immunostaining in five organs and one tumor from B6 mice. Results Plasmacytes in Peyer’s patches were positive for CD138 and Kappa markers without endogenous immunostaining. Endogenous CD138 immunostaining was widespread in liver, kidney, lung and a malignant peripheral nerve sheath tumor (MPNST) versus regionalized immunostaining in skin and small intestine wall. Endogenous Kappa immunostaining was absent in all tissues except for plasmacytes. Tissues with widespread endogenous CD138 immunostaining were contrasted by absence of endogenous Kappa immunostaining. Here, plasmacytes would not be distinguished by CD138, but would be obvious by Kappa immunostaining. Our study suggests that utility of immunostaining for plasmacytes by CD138 is tissue dependent in mice. Additionally, Kappa immunostaining may be a useful alternative in mouse tissues with confounding endogenous CD138 immunostaining.https://doi.org/10.1186/s13104-022-06100-5B-cellsCD138/syndecan-1ImmunohistochemistryKappa light chainPlasmacytes |
| spellingShingle | David K. Meyerholz Mariah R. Leidinger J. Adam Goeken Thomas R. Businga Allison Akers Sebastian Vizuett Courtney A. Kaemmer Jordan L. Kohlmeyer Rebecca D. Dodd Dawn E. Quelle Utility of CD138/syndecan-1 immunohistochemistry for localization of plasmacytes is tissue-dependent in B6 mice BMC Research Notes B-cells CD138/syndecan-1 Immunohistochemistry Kappa light chain Plasmacytes |
| title | Utility of CD138/syndecan-1 immunohistochemistry for localization of plasmacytes is tissue-dependent in B6 mice |
| title_full | Utility of CD138/syndecan-1 immunohistochemistry for localization of plasmacytes is tissue-dependent in B6 mice |
| title_fullStr | Utility of CD138/syndecan-1 immunohistochemistry for localization of plasmacytes is tissue-dependent in B6 mice |
| title_full_unstemmed | Utility of CD138/syndecan-1 immunohistochemistry for localization of plasmacytes is tissue-dependent in B6 mice |
| title_short | Utility of CD138/syndecan-1 immunohistochemistry for localization of plasmacytes is tissue-dependent in B6 mice |
| title_sort | utility of cd138 syndecan 1 immunohistochemistry for localization of plasmacytes is tissue dependent in b6 mice |
| topic | B-cells CD138/syndecan-1 Immunohistochemistry Kappa light chain Plasmacytes |
| url | https://doi.org/10.1186/s13104-022-06100-5 |
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