A Collection of Melon (<i>Cucumis melo</i>) Fruit Cultivars with Varied Skin Appearances Provide Insight to the Contribution of Suberin in Periderm Formation and Reticulation
At times of fruit skin failure, reticulation made of a wound-periderm is formed below the cracked skin in order to seal the damaged tissue. Preceding investigations shed light on the mechanisms underlying the formation of fruit skin reticulation, demonstrating that the walls of periderm cells are he...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/10/1336 |
| _version_ | 1797496407660167168 |
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| author | Ekaterina Manasherova Hagai Cohen |
| author_facet | Ekaterina Manasherova Hagai Cohen |
| author_sort | Ekaterina Manasherova |
| collection | DOAJ |
| description | At times of fruit skin failure, reticulation made of a wound-periderm is formed below the cracked skin in order to seal the damaged tissue. Preceding investigations shed light on the mechanisms underlying the formation of fruit skin reticulation, demonstrating that the walls of periderm cells are heavily suberized and lignified. However, the relative contribution of the suberin pathway to these processes, as well as the association between suberin contents in the periderm tissue and reticulation degree, are largely unknown. To strengthen our understanding on these important physiological and agricultural aspects, we comparatively profiled skin tissues of a collection of smooth- and reticulated-skin melon (<i>Cucumis melo</i>) cultivars for suberin monomer composition via gas chromatography-mass spectrometry (GC-MS). This metabolite profiling approach accompanied by statistical tools highlighted the fundamental chemical differences between the skin of smooth fruit made of a typical cuticle, to the skin of reticulated fruit made of large amounts of archetypal suberin building blocks including hydroxycinnamic acids, very long chain fatty acids, fatty alcohols, α-hydroxyacids, ω-hydroxyacids, and α,ω-diacids. Next, using image analysis we generated ‘reticulation maps’ and calculated the relative densities of reticulation. We then performed correlation assays in order to monitor suberin monomers that specifically correlate well with reticulation degree. Nonetheless, total suberin contents and most suberin building blocks did not show high correlations with reticulation degree, further suggesting that additional factors are likely to influence and regulate these processes. Altogether, the data provided vital information regarding the relative contribution of the suberin pathway to periderm formation and skin reticulation. |
| first_indexed | 2024-03-10T03:03:10Z |
| format | Article |
| id | doaj.art-e11ad0c0ecc74443b3054050507888a8 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T03:03:10Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-e11ad0c0ecc74443b3054050507888a82023-11-23T12:42:31ZengMDPI AGPlants2223-77472022-05-011110133610.3390/plants11101336A Collection of Melon (<i>Cucumis melo</i>) Fruit Cultivars with Varied Skin Appearances Provide Insight to the Contribution of Suberin in Periderm Formation and ReticulationEkaterina Manasherova0Hagai Cohen1Volcani Center, Department of Vegetable and Field Crops, Institute of Plant Sciences, Agricultural Research Organization (ARO), Rishon LeZion 7505101, IsraelVolcani Center, Department of Vegetable and Field Crops, Institute of Plant Sciences, Agricultural Research Organization (ARO), Rishon LeZion 7505101, IsraelAt times of fruit skin failure, reticulation made of a wound-periderm is formed below the cracked skin in order to seal the damaged tissue. Preceding investigations shed light on the mechanisms underlying the formation of fruit skin reticulation, demonstrating that the walls of periderm cells are heavily suberized and lignified. However, the relative contribution of the suberin pathway to these processes, as well as the association between suberin contents in the periderm tissue and reticulation degree, are largely unknown. To strengthen our understanding on these important physiological and agricultural aspects, we comparatively profiled skin tissues of a collection of smooth- and reticulated-skin melon (<i>Cucumis melo</i>) cultivars for suberin monomer composition via gas chromatography-mass spectrometry (GC-MS). This metabolite profiling approach accompanied by statistical tools highlighted the fundamental chemical differences between the skin of smooth fruit made of a typical cuticle, to the skin of reticulated fruit made of large amounts of archetypal suberin building blocks including hydroxycinnamic acids, very long chain fatty acids, fatty alcohols, α-hydroxyacids, ω-hydroxyacids, and α,ω-diacids. Next, using image analysis we generated ‘reticulation maps’ and calculated the relative densities of reticulation. We then performed correlation assays in order to monitor suberin monomers that specifically correlate well with reticulation degree. Nonetheless, total suberin contents and most suberin building blocks did not show high correlations with reticulation degree, further suggesting that additional factors are likely to influence and regulate these processes. Altogether, the data provided vital information regarding the relative contribution of the suberin pathway to periderm formation and skin reticulation.https://www.mdpi.com/2223-7747/11/10/1336melon (<i>Cucumis melo</i>)fruit skin reticulationsuberin pathwayligningas chromatography-mass spectrometry (GC-MS)very long chain fatty acids (VLCFAs) |
| spellingShingle | Ekaterina Manasherova Hagai Cohen A Collection of Melon (<i>Cucumis melo</i>) Fruit Cultivars with Varied Skin Appearances Provide Insight to the Contribution of Suberin in Periderm Formation and Reticulation Plants melon (<i>Cucumis melo</i>) fruit skin reticulation suberin pathway lignin gas chromatography-mass spectrometry (GC-MS) very long chain fatty acids (VLCFAs) |
| title | A Collection of Melon (<i>Cucumis melo</i>) Fruit Cultivars with Varied Skin Appearances Provide Insight to the Contribution of Suberin in Periderm Formation and Reticulation |
| title_full | A Collection of Melon (<i>Cucumis melo</i>) Fruit Cultivars with Varied Skin Appearances Provide Insight to the Contribution of Suberin in Periderm Formation and Reticulation |
| title_fullStr | A Collection of Melon (<i>Cucumis melo</i>) Fruit Cultivars with Varied Skin Appearances Provide Insight to the Contribution of Suberin in Periderm Formation and Reticulation |
| title_full_unstemmed | A Collection of Melon (<i>Cucumis melo</i>) Fruit Cultivars with Varied Skin Appearances Provide Insight to the Contribution of Suberin in Periderm Formation and Reticulation |
| title_short | A Collection of Melon (<i>Cucumis melo</i>) Fruit Cultivars with Varied Skin Appearances Provide Insight to the Contribution of Suberin in Periderm Formation and Reticulation |
| title_sort | collection of melon i cucumis melo i fruit cultivars with varied skin appearances provide insight to the contribution of suberin in periderm formation and reticulation |
| topic | melon (<i>Cucumis melo</i>) fruit skin reticulation suberin pathway lignin gas chromatography-mass spectrometry (GC-MS) very long chain fatty acids (VLCFAs) |
| url | https://www.mdpi.com/2223-7747/11/10/1336 |
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