Extraction (DSX) from Erigeron breviscapus modulates outward potassium currents in rat retinal ganglion cells
AIM: To investigate the effect of DSX, an active component extracted from Erigeron breviscapus, on the voltage-gated outward K+ channel currents in rat retinal ganglion cells (RGCs) by using electrophysiological method, and to explore the possible mechanisms of DSX on optic nerve protection. METHOD...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2015-12-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.ijo.cn/en_publish/2015/6/20150604.pdf |
| _version_ | 1818113450790879232 |
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| author | Shuo Yin Zhong-Feng Wang Jun-Guo Duan Lu Ji Xue-Jing Lu |
| author_facet | Shuo Yin Zhong-Feng Wang Jun-Guo Duan Lu Ji Xue-Jing Lu |
| author_sort | Shuo Yin |
| collection | DOAJ |
| description | AIM: To investigate the effect of DSX, an active component extracted from Erigeron breviscapus, on the voltage-gated outward K+ channel currents in rat retinal ganglion cells (RGCs) by using electrophysiological method, and to explore the possible mechanisms of DSX on optic nerve protection.
METHODS: Outward K+ currents were recorded by using whole-cell patch-clamp techniques on acutely isolated rat RGCs. Outward K+ currents were induced by a series of depolarizing voltage pulses from a holding potential of -70 mV to +20 mV in an increment of 10 mV.
RESULTS: Extracellular application of DSX voltage-dependently suppressed both the steady-state and peak current amplitudes of outward K+ currents in rat RGCs. Furthermore, DSX reversibly and dose-dependently inhibited the amplitudes of outward K+ currents of the cells. At +20 mV membrane potential DSX at the concentrations of 0.02 g/L and 0.05 g/L showed no significant effects on the currents. In contrast, DSX at higher concentrations (0.1 g/L, 0.2 g/L and 0.5 g/L) significantly suppressed the current amplitudes.
CONCLUSION: These results suggest that DSX reversibly and dose-dependently suppress outward K+ channel currents in rat RGCs, which may be one of the possible mechanisms underlying Erigeron breviscapus prevents vision loss and RGC damage caused by glaucoma. |
| first_indexed | 2024-12-11T03:35:02Z |
| format | Article |
| id | doaj.art-e11af032fe0e4d39886c0a3b50e1c234 |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-11T03:35:02Z |
| publishDate | 2015-12-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-e11af032fe0e4d39886c0a3b50e1c2342022-12-22T01:22:16ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982015-12-01861101110610.3980/j.issn.2222-3959.2015.06.04Extraction (DSX) from Erigeron breviscapus modulates outward potassium currents in rat retinal ganglion cellsShuo Yin0Zhong-Feng Wang1Jun-Guo Duan2Lu Ji3Xue-Jing Lu4Key Laboratory for Visual Function and Ophthalmopathy, Chengdu University of Traditional Chinese Medicine, Chengdu 610032, Sichuan Province, ChinaInstitutes of Brain Science, Fudan University, Shanghai 200032, ChinaKey Laboratory for Visual Function and Ophthalmopathy, Chengdu University of Traditional Chinese Medicine, Chengdu 610032, Sichuan Province, ChinaKey Laboratory for Visual Function and Ophthalmopathy, Chengdu University of Traditional Chinese Medicine, Chengdu 610032, Sichuan Province, ChinaKey Laboratory for Visual Function and Ophthalmopathy, Chengdu University of Traditional Chinese Medicine, Chengdu 610032, Sichuan Province, ChinaAIM: To investigate the effect of DSX, an active component extracted from Erigeron breviscapus, on the voltage-gated outward K+ channel currents in rat retinal ganglion cells (RGCs) by using electrophysiological method, and to explore the possible mechanisms of DSX on optic nerve protection. METHODS: Outward K+ currents were recorded by using whole-cell patch-clamp techniques on acutely isolated rat RGCs. Outward K+ currents were induced by a series of depolarizing voltage pulses from a holding potential of -70 mV to +20 mV in an increment of 10 mV. RESULTS: Extracellular application of DSX voltage-dependently suppressed both the steady-state and peak current amplitudes of outward K+ currents in rat RGCs. Furthermore, DSX reversibly and dose-dependently inhibited the amplitudes of outward K+ currents of the cells. At +20 mV membrane potential DSX at the concentrations of 0.02 g/L and 0.05 g/L showed no significant effects on the currents. In contrast, DSX at higher concentrations (0.1 g/L, 0.2 g/L and 0.5 g/L) significantly suppressed the current amplitudes. CONCLUSION: These results suggest that DSX reversibly and dose-dependently suppress outward K+ channel currents in rat RGCs, which may be one of the possible mechanisms underlying Erigeron breviscapus prevents vision loss and RGC damage caused by glaucoma.http://www.ijo.cn/en_publish/2015/6/20150604.pdfErigeron breviscapusretinal ganglion cellspotassium channel |
| spellingShingle | Shuo Yin Zhong-Feng Wang Jun-Guo Duan Lu Ji Xue-Jing Lu Extraction (DSX) from Erigeron breviscapus modulates outward potassium currents in rat retinal ganglion cells International Journal of Ophthalmology Erigeron breviscapus retinal ganglion cells potassium channel |
| title | Extraction (DSX) from Erigeron breviscapus modulates outward potassium currents in rat retinal ganglion cells |
| title_full | Extraction (DSX) from Erigeron breviscapus modulates outward potassium currents in rat retinal ganglion cells |
| title_fullStr | Extraction (DSX) from Erigeron breviscapus modulates outward potassium currents in rat retinal ganglion cells |
| title_full_unstemmed | Extraction (DSX) from Erigeron breviscapus modulates outward potassium currents in rat retinal ganglion cells |
| title_short | Extraction (DSX) from Erigeron breviscapus modulates outward potassium currents in rat retinal ganglion cells |
| title_sort | extraction dsx from erigeron breviscapus modulates outward potassium currents in rat retinal ganglion cells |
| topic | Erigeron breviscapus retinal ganglion cells potassium channel |
| url | http://www.ijo.cn/en_publish/2015/6/20150604.pdf |
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