Colchicine did not affect the viability of induced 2n pollen in Populus tomentosa
Colchicine is widely used as a mutagen to induce production of diploid gametes in plants. However, whether colchicine affects induced pollen viability remains unclear. To clarify whether colchicine affected the viability of induced pollen, we induced production of diploid pollen by colchicine, follo...
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| Format: | Article |
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Finnish Society of Forest Science
2019-06-01
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| Series: | Silva Fennica |
| Subjects: |
| _version_ | 1818389134811594752 |
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| author | Yan Liu Yuan Zhang Qing Zhou Jian Wu Pingdong Zhang |
| author_facet | Yan Liu Yuan Zhang Qing Zhou Jian Wu Pingdong Zhang |
| author_sort | Yan Liu |
| collection | DOAJ |
| description | Colchicine is widely used as a mutagen to induce production of diploid gametes in plants. However, whether colchicine affects induced pollen viability remains unclear. To clarify whether colchicine affected the viability of induced pollen, we induced production of diploid pollen by colchicine, followed by pollen germination in vitro and crossing induced pollen with normal gametes to produce triploid in Populus tomentosa Carrière. The results showed that the predominant meiotic stages and the number of colchicine injections had significant effects on the occurrence rates of induced 2n pollen. When the colchicine injection was given at diakinesis, a significant decrease in the pollen production per bud was observed (p < 0.001). The morphology of the colchicine-induced 2n pollen was similar to that of the natural 2n pollen in its ectexine structure. The pollen germination experiments revealed that there was also no significant difference in germination rates between the induced diploid pollen and natural 2n pollen grains, and 68 triploids were created by crossing colchicine-induced pollen. Our findings revealed that colchicine injection could induce P. tomentosa to produce 2n pollen and will not lead to dysfunction of induced diploid pollen. |
| first_indexed | 2024-12-14T04:36:55Z |
| format | Article |
| id | doaj.art-e11b32ffe9534575a8bd771c1e7291c1 |
| institution | Directory Open Access Journal |
| issn | 2242-4075 2242-4075 |
| language | English |
| last_indexed | 2024-12-14T04:36:55Z |
| publishDate | 2019-06-01 |
| publisher | Finnish Society of Forest Science |
| record_format | Article |
| series | Silva Fennica |
| spelling | doaj.art-e11b32ffe9534575a8bd771c1e7291c12022-12-21T23:16:55ZengFinnish Society of Forest ScienceSilva Fennica2242-40752242-40752019-06-0153210.14214/sf.10132Colchicine did not affect the viability of induced 2n pollen in Populus tomentosaYan Liu0Yuan Zhang1Qing Zhou2Jian Wu3Pingdong Zhang4National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; College of Bioscience and Biotechnology, Beijing Forestry University, Beijing 100083, ChinaNational Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; College of Bioscience and Biotechnology, Beijing Forestry University, Beijing 100083, ChinaNational Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; College of Bioscience and Biotechnology, Beijing Forestry University, Beijing 100083, ChinaNational Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; College of Bioscience and Biotechnology, Beijing Forestry University, Beijing 100083, ChinaNational Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; College of Bioscience and Biotechnology, Beijing Forestry University, Beijing 100083, ChinaColchicine is widely used as a mutagen to induce production of diploid gametes in plants. However, whether colchicine affects induced pollen viability remains unclear. To clarify whether colchicine affected the viability of induced pollen, we induced production of diploid pollen by colchicine, followed by pollen germination in vitro and crossing induced pollen with normal gametes to produce triploid in Populus tomentosa Carrière. The results showed that the predominant meiotic stages and the number of colchicine injections had significant effects on the occurrence rates of induced 2n pollen. When the colchicine injection was given at diakinesis, a significant decrease in the pollen production per bud was observed (p < 0.001). The morphology of the colchicine-induced 2n pollen was similar to that of the natural 2n pollen in its ectexine structure. The pollen germination experiments revealed that there was also no significant difference in germination rates between the induced diploid pollen and natural 2n pollen grains, and 68 triploids were created by crossing colchicine-induced pollen. Our findings revealed that colchicine injection could induce P. tomentosa to produce 2n pollen and will not lead to dysfunction of induced diploid pollen.colchicine-induced 2n pollenpollen productionpollen viabilitypollen germination ratetriploid |
| spellingShingle | Yan Liu Yuan Zhang Qing Zhou Jian Wu Pingdong Zhang Colchicine did not affect the viability of induced 2n pollen in Populus tomentosa Silva Fennica colchicine-induced 2n pollen pollen production pollen viability pollen germination rate triploid |
| title | Colchicine did not affect the viability of induced 2n pollen in Populus tomentosa |
| title_full | Colchicine did not affect the viability of induced 2n pollen in Populus tomentosa |
| title_fullStr | Colchicine did not affect the viability of induced 2n pollen in Populus tomentosa |
| title_full_unstemmed | Colchicine did not affect the viability of induced 2n pollen in Populus tomentosa |
| title_short | Colchicine did not affect the viability of induced 2n pollen in Populus tomentosa |
| title_sort | colchicine did not affect the viability of induced 2n pollen in populus tomentosa |
| topic | colchicine-induced 2n pollen pollen production pollen viability pollen germination rate triploid |
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