Bletilla striata polysaccharides ameliorates lipopolysaccharide-induced injury in intestinal epithelial cells
Background/Aims: This study was carried out to investigate the effect of Bletilla striata polysaccharide (BSP) treating on lipopolysaccharide (LPS)-induced intestinal epithelial barrier disruption in rat intestinal epithelial cell (IEC) line. Materials and Methods: LPS was used to stimulate the IEC-...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | The Saudi Journal of Gastroenterology |
| Subjects: | |
| Online Access: | http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2019;volume=25;issue=5;spage=302;epage=308;aulast=Luo |
| _version_ | 1828789634258173952 |
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| author | Lei Luo Yuqing Liu Xin Cai Yao Wang Juan Xue Juan Zhang Fan Yang |
| author_facet | Lei Luo Yuqing Liu Xin Cai Yao Wang Juan Xue Juan Zhang Fan Yang |
| author_sort | Lei Luo |
| collection | DOAJ |
| description | Background/Aims: This study was carried out to investigate the effect of Bletilla striata polysaccharide (BSP) treating on lipopolysaccharide (LPS)-induced intestinal epithelial barrier disruption in rat intestinal epithelial cell (IEC) line.
Materials and Methods: LPS was used to stimulate the IEC-18 cells (1 μg/ml), with or without different concentrations of BSP (25, 50 and 100 μg/ml) for 24 h. Transepithelial electrical resistance (TEER) was measured to detect the permeability of cells. Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the cell supernatant were detected with the enzyme-linked immunosorbent assay (ELISA). Real-time polymerase chain reaction (PCR) was employed to detect the mRNA levels of zonulae occludens (ZO)-1 and occludin. Western blot and immunofluorescence analysis were used for analyzing the expression level and the distribution patterns of ZO-1 and occludin protein.
Results: After treatment with BSP, the IL-6 and TNF-α levels in the cell supernatant were significantly decreased compared with the experiment group (P < 0.05 or 0.01). The permeability of IEC was decreased in BSP groups when compared with the experiment group (P < 0.05 or 0.01). In addition, compared with the experiment group, treatment with BSP up-regulated mRNA and protein expression levels of ZO-1 and occludin, and kept the ZO-1 and occludin protein intact in IEC-18 cells injured with LPS (P < 0.05 or 0.01).
Conclusion: BSP has the capacity to protect IEC-18 cells from LPS-induced injury. The mechanisms may be associated with decreasing the inflammatory cytokine levels of IL-6 and TNF-α, and elevating the expression of ZO-1 and occludin, which might serve as a new protective agent for LPS-induced intestinal epithelial barrier disruption. |
| first_indexed | 2024-12-12T01:16:02Z |
| format | Article |
| id | doaj.art-e123fdec620b4bcfac1cab3decda01f5 |
| institution | Directory Open Access Journal |
| issn | 1319-3767 1998-4049 |
| language | English |
| last_indexed | 2024-12-12T01:16:02Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | The Saudi Journal of Gastroenterology |
| spelling | doaj.art-e123fdec620b4bcfac1cab3decda01f52022-12-22T00:43:21ZengWolters Kluwer Medknow PublicationsThe Saudi Journal of Gastroenterology1319-37671998-40492019-01-0125530230810.4103/sjg.SJG_520_18Bletilla striata polysaccharides ameliorates lipopolysaccharide-induced injury in intestinal epithelial cellsLei LuoYuqing LiuXin CaiYao WangJuan XueJuan ZhangFan YangBackground/Aims: This study was carried out to investigate the effect of Bletilla striata polysaccharide (BSP) treating on lipopolysaccharide (LPS)-induced intestinal epithelial barrier disruption in rat intestinal epithelial cell (IEC) line. Materials and Methods: LPS was used to stimulate the IEC-18 cells (1 μg/ml), with or without different concentrations of BSP (25, 50 and 100 μg/ml) for 24 h. Transepithelial electrical resistance (TEER) was measured to detect the permeability of cells. Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the cell supernatant were detected with the enzyme-linked immunosorbent assay (ELISA). Real-time polymerase chain reaction (PCR) was employed to detect the mRNA levels of zonulae occludens (ZO)-1 and occludin. Western blot and immunofluorescence analysis were used for analyzing the expression level and the distribution patterns of ZO-1 and occludin protein. Results: After treatment with BSP, the IL-6 and TNF-α levels in the cell supernatant were significantly decreased compared with the experiment group (P < 0.05 or 0.01). The permeability of IEC was decreased in BSP groups when compared with the experiment group (P < 0.05 or 0.01). In addition, compared with the experiment group, treatment with BSP up-regulated mRNA and protein expression levels of ZO-1 and occludin, and kept the ZO-1 and occludin protein intact in IEC-18 cells injured with LPS (P < 0.05 or 0.01). Conclusion: BSP has the capacity to protect IEC-18 cells from LPS-induced injury. The mechanisms may be associated with decreasing the inflammatory cytokine levels of IL-6 and TNF-α, and elevating the expression of ZO-1 and occludin, which might serve as a new protective agent for LPS-induced intestinal epithelial barrier disruption.http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2019;volume=25;issue=5;spage=302;epage=308;aulast=LuoBletilla striata polysaccharideIEC-18 cellsoccludinZO-1 |
| spellingShingle | Lei Luo Yuqing Liu Xin Cai Yao Wang Juan Xue Juan Zhang Fan Yang Bletilla striata polysaccharides ameliorates lipopolysaccharide-induced injury in intestinal epithelial cells The Saudi Journal of Gastroenterology Bletilla striata polysaccharide IEC-18 cells occludin ZO-1 |
| title | Bletilla striata polysaccharides ameliorates lipopolysaccharide-induced injury in intestinal epithelial cells |
| title_full | Bletilla striata polysaccharides ameliorates lipopolysaccharide-induced injury in intestinal epithelial cells |
| title_fullStr | Bletilla striata polysaccharides ameliorates lipopolysaccharide-induced injury in intestinal epithelial cells |
| title_full_unstemmed | Bletilla striata polysaccharides ameliorates lipopolysaccharide-induced injury in intestinal epithelial cells |
| title_short | Bletilla striata polysaccharides ameliorates lipopolysaccharide-induced injury in intestinal epithelial cells |
| title_sort | bletilla striata polysaccharides ameliorates lipopolysaccharide induced injury in intestinal epithelial cells |
| topic | Bletilla striata polysaccharide IEC-18 cells occludin ZO-1 |
| url | http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2019;volume=25;issue=5;spage=302;epage=308;aulast=Luo |
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