Development of Microscopy Apparatus Switchable between Fluorescence and Ultralow-Frequency Raman Modes
In this study, a microscopy apparatus that can switch between the fluorescence microscopy and ultralow-frequency Raman microscopy modes was developed. The apparatus can be easily constructed by equipping a standard epi-illumination microscope with an additional port featuring a removable half mirror...
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| Format: | Article |
| Language: | English |
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Hindawi Limited
2022-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2022/2694545 |
| _version_ | 1811181871873130496 |
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| author | Ken Takazawa |
| author_facet | Ken Takazawa |
| author_sort | Ken Takazawa |
| collection | DOAJ |
| description | In this study, a microscopy apparatus that can switch between the fluorescence microscopy and ultralow-frequency Raman microscopy modes was developed. The apparatus can be easily constructed by equipping a standard epi-illumination microscope with an additional port featuring a removable half mirror. Owing to the switchability, fluorescence imaging, and spectroscopy, Raman spectroscopy in the frequency range down to ∼10 cm−1 can be performed using the apparatus. To demonstrate the advantageous features of this apparatus, micron-sized crystals of perylene, which have two polymorphic forms, were analyzed. The two polymorphs were clearly identified based on their shapes, fluorescence spectra, and ultralow-frequency Raman spectra, all of which can be observed with our apparatus alone. These results indicate that the apparatus is a powerful tool for the analysis and characterization of various nano-/micron-sized crystals. |
| first_indexed | 2024-04-11T09:24:07Z |
| format | Article |
| id | doaj.art-e1261d63a5af41e498ae3493d3b0429a |
| institution | Directory Open Access Journal |
| issn | 2090-8873 |
| language | English |
| last_indexed | 2024-04-11T09:24:07Z |
| publishDate | 2022-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj.art-e1261d63a5af41e498ae3493d3b0429a2022-12-22T04:32:06ZengHindawi LimitedJournal of Analytical Methods in Chemistry2090-88732022-01-01202210.1155/2022/2694545Development of Microscopy Apparatus Switchable between Fluorescence and Ultralow-Frequency Raman ModesKen Takazawa0National Institute for Materials ScienceIn this study, a microscopy apparatus that can switch between the fluorescence microscopy and ultralow-frequency Raman microscopy modes was developed. The apparatus can be easily constructed by equipping a standard epi-illumination microscope with an additional port featuring a removable half mirror. Owing to the switchability, fluorescence imaging, and spectroscopy, Raman spectroscopy in the frequency range down to ∼10 cm−1 can be performed using the apparatus. To demonstrate the advantageous features of this apparatus, micron-sized crystals of perylene, which have two polymorphic forms, were analyzed. The two polymorphs were clearly identified based on their shapes, fluorescence spectra, and ultralow-frequency Raman spectra, all of which can be observed with our apparatus alone. These results indicate that the apparatus is a powerful tool for the analysis and characterization of various nano-/micron-sized crystals.http://dx.doi.org/10.1155/2022/2694545 |
| spellingShingle | Ken Takazawa Development of Microscopy Apparatus Switchable between Fluorescence and Ultralow-Frequency Raman Modes Journal of Analytical Methods in Chemistry |
| title | Development of Microscopy Apparatus Switchable between Fluorescence and Ultralow-Frequency Raman Modes |
| title_full | Development of Microscopy Apparatus Switchable between Fluorescence and Ultralow-Frequency Raman Modes |
| title_fullStr | Development of Microscopy Apparatus Switchable between Fluorescence and Ultralow-Frequency Raman Modes |
| title_full_unstemmed | Development of Microscopy Apparatus Switchable between Fluorescence and Ultralow-Frequency Raman Modes |
| title_short | Development of Microscopy Apparatus Switchable between Fluorescence and Ultralow-Frequency Raman Modes |
| title_sort | development of microscopy apparatus switchable between fluorescence and ultralow frequency raman modes |
| url | http://dx.doi.org/10.1155/2022/2694545 |
| work_keys_str_mv | AT kentakazawa developmentofmicroscopyapparatusswitchablebetweenfluorescenceandultralowfrequencyramanmodes |