High clonality of Mycobacterium avium subsp. paratuberculosis field isolates from red deer revealed by two different methodological approaches of comparative genomic analysis
Mycobacterium avium subsp. paratuberculosis (MAP) is the aetiological agent of paratuberculosis (Johne’s disease) in both domestic and wild ruminants. In the present study, using a whole-genome sequence (WGS) approach, we investigated the genetic diversity of 15 Mycobacterium avium field strains iso...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1301667/full |
| _version_ | 1797323718205112320 |
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| author | Silvia Turco Simone Russo Daniele Pietrucci Anita Filippi Marco Milanesi Camilla Luzzago Chiara Garbarino Giorgia Palladini Giovanni Chillemi Giovanni Chillemi Matteo Ricchi |
| author_facet | Silvia Turco Simone Russo Daniele Pietrucci Anita Filippi Marco Milanesi Camilla Luzzago Chiara Garbarino Giorgia Palladini Giovanni Chillemi Giovanni Chillemi Matteo Ricchi |
| author_sort | Silvia Turco |
| collection | DOAJ |
| description | Mycobacterium avium subsp. paratuberculosis (MAP) is the aetiological agent of paratuberculosis (Johne’s disease) in both domestic and wild ruminants. In the present study, using a whole-genome sequence (WGS) approach, we investigated the genetic diversity of 15 Mycobacterium avium field strains isolated in the last 10 years from red deer inhabiting the Stelvio National Park and affected by paratuberculosis. Combining de novo assembly and a reference-based method, followed by a pangenome analysis, we highlight a very close relationship among 13 MAP field isolates, suggesting that a single infecting event occurred in this population. Moreover, two isolates have been classified as Mycobacterium avium subsp. hominissuis, distinct from the other MAPs under comparison but close to each other. This is the first time that this subspecies has been found in Italy in samples without evident epidemiological correlations, having been isolated in two different locations of the Stelvio National Park and in different years. Our study highlights the importance of a multidisciplinary approach incorporating molecular epidemiology and ecology into traditional infectious disease knowledge in order to investigate the nature of infectious disease in wildlife populations. |
| first_indexed | 2024-03-08T05:32:30Z |
| format | Article |
| id | doaj.art-e1276530c20f411ea45f36349a4b2aa0 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-08T05:32:30Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-e1276530c20f411ea45f36349a4b2aa02024-02-06T04:46:47ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-02-011110.3389/fvets.2024.13016671301667High clonality of Mycobacterium avium subsp. paratuberculosis field isolates from red deer revealed by two different methodological approaches of comparative genomic analysisSilvia Turco0Simone Russo1Daniele Pietrucci2Anita Filippi3Marco Milanesi4Camilla Luzzago5Chiara Garbarino6Giorgia Palladini7Giovanni Chillemi8Giovanni Chillemi9Matteo Ricchi10Dipartimento di Scienze Agrarie e Forestali (DAFNE), Università degli Studi della Tuscia, Viterbo, ItalyNational Reference Centre and WOAH Reference Laboratory for Paratuberculosis, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna “Bruno Ubertini”, Piacenza, ItalyDipartimento per l'Innovazione nei Sistemi Biologici, Agroalimentari e Forestali (DIBAF), Università degli Studi della Tuscia, Viterbo, ItalyNational Reference Centre and WOAH Reference Laboratory for Paratuberculosis, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna “Bruno Ubertini”, Piacenza, ItalyDipartimento per l'Innovazione nei Sistemi Biologici, Agroalimentari e Forestali (DIBAF), Università degli Studi della Tuscia, Viterbo, ItalyDepartment of Veterinary Medicine and Animal Sciences, Coordinated Research Centre "EpiSoMI", University of Milan, Lodi, ItalyNational Reference Centre and WOAH Reference Laboratory for Paratuberculosis, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna “Bruno Ubertini”, Piacenza, ItalyNational Reference Centre and WOAH Reference Laboratory for Paratuberculosis, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna “Bruno Ubertini”, Piacenza, ItalyDipartimento per l'Innovazione nei Sistemi Biologici, Agroalimentari e Forestali (DIBAF), Università degli Studi della Tuscia, Viterbo, ItalyInstitute of Translational Pharmacology, National Research Council, CNR, Rome, ItalyNational Reference Centre and WOAH Reference Laboratory for Paratuberculosis, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna “Bruno Ubertini”, Piacenza, ItalyMycobacterium avium subsp. paratuberculosis (MAP) is the aetiological agent of paratuberculosis (Johne’s disease) in both domestic and wild ruminants. In the present study, using a whole-genome sequence (WGS) approach, we investigated the genetic diversity of 15 Mycobacterium avium field strains isolated in the last 10 years from red deer inhabiting the Stelvio National Park and affected by paratuberculosis. Combining de novo assembly and a reference-based method, followed by a pangenome analysis, we highlight a very close relationship among 13 MAP field isolates, suggesting that a single infecting event occurred in this population. Moreover, two isolates have been classified as Mycobacterium avium subsp. hominissuis, distinct from the other MAPs under comparison but close to each other. This is the first time that this subspecies has been found in Italy in samples without evident epidemiological correlations, having been isolated in two different locations of the Stelvio National Park and in different years. Our study highlights the importance of a multidisciplinary approach incorporating molecular epidemiology and ecology into traditional infectious disease knowledge in order to investigate the nature of infectious disease in wildlife populations.https://www.frontiersin.org/articles/10.3389/fvets.2024.1301667/fullMycobacterium avium subsp. paratuberculosisred deerwild animalsItalywhole genome sequencingepidemiology |
| spellingShingle | Silvia Turco Simone Russo Daniele Pietrucci Anita Filippi Marco Milanesi Camilla Luzzago Chiara Garbarino Giorgia Palladini Giovanni Chillemi Giovanni Chillemi Matteo Ricchi High clonality of Mycobacterium avium subsp. paratuberculosis field isolates from red deer revealed by two different methodological approaches of comparative genomic analysis Frontiers in Veterinary Science Mycobacterium avium subsp. paratuberculosis red deer wild animals Italy whole genome sequencing epidemiology |
| title | High clonality of Mycobacterium avium subsp. paratuberculosis field isolates from red deer revealed by two different methodological approaches of comparative genomic analysis |
| title_full | High clonality of Mycobacterium avium subsp. paratuberculosis field isolates from red deer revealed by two different methodological approaches of comparative genomic analysis |
| title_fullStr | High clonality of Mycobacterium avium subsp. paratuberculosis field isolates from red deer revealed by two different methodological approaches of comparative genomic analysis |
| title_full_unstemmed | High clonality of Mycobacterium avium subsp. paratuberculosis field isolates from red deer revealed by two different methodological approaches of comparative genomic analysis |
| title_short | High clonality of Mycobacterium avium subsp. paratuberculosis field isolates from red deer revealed by two different methodological approaches of comparative genomic analysis |
| title_sort | high clonality of mycobacterium avium subsp paratuberculosis field isolates from red deer revealed by two different methodological approaches of comparative genomic analysis |
| topic | Mycobacterium avium subsp. paratuberculosis red deer wild animals Italy whole genome sequencing epidemiology |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1301667/full |
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