A Microchip for High-Throughput Axon Growth Drug Screening
It has been recently known that not only the presence of inhibitory molecules associated with myelin but also the reduced growth capability of the axons limit mature central nervous system (CNS) axonal regeneration after injury. Conventional axon growth studies are typically conducted using multi-we...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-07-01
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| Series: | Micromachines |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2072-666X/7/7/114 |
| _version_ | 1818621036277530624 |
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| author | Hyun Soo Kim Sehoon Jeong Chiwan Koo Arum Han Jaewon Park |
| author_facet | Hyun Soo Kim Sehoon Jeong Chiwan Koo Arum Han Jaewon Park |
| author_sort | Hyun Soo Kim |
| collection | DOAJ |
| description | It has been recently known that not only the presence of inhibitory molecules associated with myelin but also the reduced growth capability of the axons limit mature central nervous system (CNS) axonal regeneration after injury. Conventional axon growth studies are typically conducted using multi-well cell culture plates that are very difficult to use for investigating localized effects of drugs and limited to low throughput. Unfortunately, there is currently no other in vitro tool that allows investigating localized axonal responses to biomolecules in high-throughput for screening potential drugs that might promote axonal growth. We have developed a compartmentalized neuron culture platform enabling localized biomolecular treatments in parallel to axons that are physically and fluidically isolated from their neuronal somata. The 24 axon compartments in the developed platform are designed to perform four sets of six different localized biomolecular treatments simultaneously on a single device. In addition, the novel microfluidic configuration allows culture medium of 24 axon compartments to be replenished altogether by a single aspiration process, making high-throughput drug screening a reality. |
| first_indexed | 2024-12-16T18:02:53Z |
| format | Article |
| id | doaj.art-e127cc46178a40af83b0b2a4b9b5632b |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-12-16T18:02:53Z |
| publishDate | 2016-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-e127cc46178a40af83b0b2a4b9b5632b2022-12-21T22:22:01ZengMDPI AGMicromachines2072-666X2016-07-017711410.3390/mi7070114mi7070114A Microchip for High-Throughput Axon Growth Drug ScreeningHyun Soo Kim0Sehoon Jeong1Chiwan Koo2Arum Han3Jaewon Park4Department of Electrical and Computer Engineering, Texas A&M University, College Station, TX 77843, USADepartment of Biomedical Engineering, Texas A&M University, College Station, TX 77843, USADepartment of Electronics and Control Engineering, Hanbat National University, Daejeon 305-719, KoreaDepartment of Electrical and Computer Engineering, Texas A&M University, College Station, TX 77843, USADepartment of Electrical and Computer Engineering, Texas A&M University, College Station, TX 77843, USAIt has been recently known that not only the presence of inhibitory molecules associated with myelin but also the reduced growth capability of the axons limit mature central nervous system (CNS) axonal regeneration after injury. Conventional axon growth studies are typically conducted using multi-well cell culture plates that are very difficult to use for investigating localized effects of drugs and limited to low throughput. Unfortunately, there is currently no other in vitro tool that allows investigating localized axonal responses to biomolecules in high-throughput for screening potential drugs that might promote axonal growth. We have developed a compartmentalized neuron culture platform enabling localized biomolecular treatments in parallel to axons that are physically and fluidically isolated from their neuronal somata. The 24 axon compartments in the developed platform are designed to perform four sets of six different localized biomolecular treatments simultaneously on a single device. In addition, the novel microfluidic configuration allows culture medium of 24 axon compartments to be replenished altogether by a single aspiration process, making high-throughput drug screening a reality.http://www.mdpi.com/2072-666X/7/7/114microfluidic neuron culture platformcompartmentalized culturelocalized biomolecular treatmenthigh-throughput screening |
| spellingShingle | Hyun Soo Kim Sehoon Jeong Chiwan Koo Arum Han Jaewon Park A Microchip for High-Throughput Axon Growth Drug Screening Micromachines microfluidic neuron culture platform compartmentalized culture localized biomolecular treatment high-throughput screening |
| title | A Microchip for High-Throughput Axon Growth Drug Screening |
| title_full | A Microchip for High-Throughput Axon Growth Drug Screening |
| title_fullStr | A Microchip for High-Throughput Axon Growth Drug Screening |
| title_full_unstemmed | A Microchip for High-Throughput Axon Growth Drug Screening |
| title_short | A Microchip for High-Throughput Axon Growth Drug Screening |
| title_sort | microchip for high throughput axon growth drug screening |
| topic | microfluidic neuron culture platform compartmentalized culture localized biomolecular treatment high-throughput screening |
| url | http://www.mdpi.com/2072-666X/7/7/114 |
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