Biological activities and DNA interactions of aqueous extract of Phlomis linearis (Boiss. & Bal.)
Phlomis linearis Boiss. & Bal. of the Lamiaceae family is one of the endemic species in Turkey, i.e., growing in the east, central, and southeast parts of Anatolia and used for herbal tea. This study was designed to identify the biochemical and bioactivity properties of this endemic species by D...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bektas Tepe
2023-03-01
|
| Series: | International Journal of Plant Based Pharmaceuticals |
| Subjects: | |
| Online Access: | https://ijpbp.com/index.php/ijpbp/article/view/75 |
| _version_ | 1827875120972038144 |
|---|---|
| author | Betül Aydın Lütfiye Yasemin Gönder Nebahat Aytuna Çerçi Yiğit Can Ateş İlayda Sezin Yalçınkaya Nüveyre Canbolat Leyla Açık Nurcan Karacan Emine Akyüz Turumtay Halbay Turumtay |
| author_facet | Betül Aydın Lütfiye Yasemin Gönder Nebahat Aytuna Çerçi Yiğit Can Ateş İlayda Sezin Yalçınkaya Nüveyre Canbolat Leyla Açık Nurcan Karacan Emine Akyüz Turumtay Halbay Turumtay |
| author_sort | Betül Aydın |
| collection | DOAJ |
| description | Phlomis linearis Boiss. & Bal. of the Lamiaceae family is one of the endemic species in Turkey, i.e., growing in the east, central, and southeast parts of Anatolia and used for herbal tea. This study was designed to identify the biochemical and bioactivity properties of this endemic species by DPPH scavenging activity, metal chelating activity, total phenolic content, HPLC-DAD analysis, and MTT assay. Furthermore, the plant extract was evaluated for its antimicrobial activity against bacteria and fungi by using the microdilution method. The interactions between extract and plasmid DNA and their restriction endonuclease reactions were investigated by agarose gel electrophoresis. To support our hypothesis, we performed a molecular docking analysis. The DPPH scavenging activity of the plant extract was 53.86 ± 0.50 µg/ml in terms of IC50 value. The IC50 value of the plant extract was determined as 14.71 ± 4.01 mg/ml for metal chelating assay. The phenolic content of the extract was 231.55 ± 2.11 mg/g dry weight expressed as gallic acid equivalents (GAE). HPLC-DAD results revealed that the phenolic compounds were mainly derivatives of rosmarinic acid, chlorogenic acid, luteolin, luteolin-7-glycoside, luteolin derivatives, rutin derivatives, and apigenin derivatives. Besides, the cytotoxic activity of the plant extract against L929 fibroblast, H1299 non-small-cell lung carcinoma, and Caco-2 colorectal adenocarcinoma cell lines was determined by MTT assay. Phenolic content and molecular docking results correlated with each other. |
| first_indexed | 2024-03-12T17:02:22Z |
| format | Article |
| id | doaj.art-e128e55ff9d94cc3bb91c9fcc76934b6 |
| institution | Directory Open Access Journal |
| issn | 2791-7509 |
| language | English |
| last_indexed | 2024-03-12T17:02:22Z |
| publishDate | 2023-03-01 |
| publisher | Bektas Tepe |
| record_format | Article |
| series | International Journal of Plant Based Pharmaceuticals |
| spelling | doaj.art-e128e55ff9d94cc3bb91c9fcc76934b62023-08-07T09:56:15ZengBektas TepeInternational Journal of Plant Based Pharmaceuticals2791-75092023-03-0131738510.29228/ijpbp.1975Biological activities and DNA interactions of aqueous extract of Phlomis linearis (Boiss. & Bal.)Betül Aydın0https://orcid.org/0000-0002-9092-1350Lütfiye Yasemin Gönder1https://orcid.org/0000-0001-6849-3364Nebahat Aytuna Çerçi2https://orcid.org/0000-0002-7864-7213Yiğit Can Ateş3https://orcid.org/0000-0002-4564-987Xİlayda Sezin Yalçınkaya4https://orcid.org/0000-0002-8746-2528Nüveyre Canbolat5https://orcid.org/0000-0003-4466-4602Leyla Açık6https://orcid.org/0000-0002-3672-8429Nurcan Karacan7https://orcid.org/0000-0002-9273-7987Emine Akyüz Turumtay8https://orcid.org/0000-0002-1504-4810Halbay Turumtay9https://orcid.org/0000-0003-4224-8103Gazi University, Faculty of Science, Department of Biology, Ankara, TürkiyeThe Turkish Sugar Authority, Ankara, TürkiyeKırıkkale University, Scientific and Technological Research Application and Research Center, Kırıkkale, TürkiyeKoç University, Faculty of Science, Department of Molecular Biology and Genetics, İstanbul, TürkiyeGazi University, Faculty of Science, Department of Biology, Ankara, TürkiyeGazi University, Faculty of Science, Department of Chemistry, Ankara, TürkiyeGazi University, Faculty of Science, Department of Biology, Ankara, TürkiyeGazi University, Faculty of Science, Department of Chemistry, Ankara, TürkiyeRecep Tayyip Erdogan University, Faculty of Science, Department of Chemistry, Rize, TürkiyeKaradeniz Technical University, Faculty of Technology, Department of Energy Systems Engineering, Trabzon, TürkiyePhlomis linearis Boiss. & Bal. of the Lamiaceae family is one of the endemic species in Turkey, i.e., growing in the east, central, and southeast parts of Anatolia and used for herbal tea. This study was designed to identify the biochemical and bioactivity properties of this endemic species by DPPH scavenging activity, metal chelating activity, total phenolic content, HPLC-DAD analysis, and MTT assay. Furthermore, the plant extract was evaluated for its antimicrobial activity against bacteria and fungi by using the microdilution method. The interactions between extract and plasmid DNA and their restriction endonuclease reactions were investigated by agarose gel electrophoresis. To support our hypothesis, we performed a molecular docking analysis. The DPPH scavenging activity of the plant extract was 53.86 ± 0.50 µg/ml in terms of IC50 value. The IC50 value of the plant extract was determined as 14.71 ± 4.01 mg/ml for metal chelating assay. The phenolic content of the extract was 231.55 ± 2.11 mg/g dry weight expressed as gallic acid equivalents (GAE). HPLC-DAD results revealed that the phenolic compounds were mainly derivatives of rosmarinic acid, chlorogenic acid, luteolin, luteolin-7-glycoside, luteolin derivatives, rutin derivatives, and apigenin derivatives. Besides, the cytotoxic activity of the plant extract against L929 fibroblast, H1299 non-small-cell lung carcinoma, and Caco-2 colorectal adenocarcinoma cell lines was determined by MTT assay. Phenolic content and molecular docking results correlated with each other.https://ijpbp.com/index.php/ijpbp/article/view/75phlomis linearisbiological activitycytotoxicitydna interactionmolecular docking |
| spellingShingle | Betül Aydın Lütfiye Yasemin Gönder Nebahat Aytuna Çerçi Yiğit Can Ateş İlayda Sezin Yalçınkaya Nüveyre Canbolat Leyla Açık Nurcan Karacan Emine Akyüz Turumtay Halbay Turumtay Biological activities and DNA interactions of aqueous extract of Phlomis linearis (Boiss. & Bal.) International Journal of Plant Based Pharmaceuticals phlomis linearis biological activity cytotoxicity dna interaction molecular docking |
| title | Biological activities and DNA interactions of aqueous extract of Phlomis linearis (Boiss. & Bal.) |
| title_full | Biological activities and DNA interactions of aqueous extract of Phlomis linearis (Boiss. & Bal.) |
| title_fullStr | Biological activities and DNA interactions of aqueous extract of Phlomis linearis (Boiss. & Bal.) |
| title_full_unstemmed | Biological activities and DNA interactions of aqueous extract of Phlomis linearis (Boiss. & Bal.) |
| title_short | Biological activities and DNA interactions of aqueous extract of Phlomis linearis (Boiss. & Bal.) |
| title_sort | biological activities and dna interactions of aqueous extract of phlomis linearis boiss bal |
| topic | phlomis linearis biological activity cytotoxicity dna interaction molecular docking |
| url | https://ijpbp.com/index.php/ijpbp/article/view/75 |
| work_keys_str_mv | AT betulaydın biologicalactivitiesanddnainteractionsofaqueousextractofphlomislinearisboissbal AT lutfiyeyasemingonder biologicalactivitiesanddnainteractionsofaqueousextractofphlomislinearisboissbal AT nebahataytunacerci biologicalactivitiesanddnainteractionsofaqueousextractofphlomislinearisboissbal AT yigitcanates biologicalactivitiesanddnainteractionsofaqueousextractofphlomislinearisboissbal AT ilaydasezinyalcınkaya biologicalactivitiesanddnainteractionsofaqueousextractofphlomislinearisboissbal AT nuveyrecanbolat biologicalactivitiesanddnainteractionsofaqueousextractofphlomislinearisboissbal AT leylaacık biologicalactivitiesanddnainteractionsofaqueousextractofphlomislinearisboissbal AT nurcankaracan biologicalactivitiesanddnainteractionsofaqueousextractofphlomislinearisboissbal AT emineakyuzturumtay biologicalactivitiesanddnainteractionsofaqueousextractofphlomislinearisboissbal AT halbayturumtay biologicalactivitiesanddnainteractionsofaqueousextractofphlomislinearisboissbal |