Oxytocin reduces adipose tissue inflammation in obese mice
Abstract Background Obesity and adipose tissue expansion is characterized by a chronic state of systemic inflammation that contributes to disease. The neuropeptide, oxytocin, working through its receptor has been shown to attenuate inflammation in sepsis, wound healing, and cardiovascular disease. T...
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| Format: | Article |
| Language: | English |
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BMC
2020-08-01
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| Series: | Lipids in Health and Disease |
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| Online Access: | http://link.springer.com/article/10.1186/s12944-020-01364-x |
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| author | Angela Szeto Monia Cecati Raisa Ahmed Philip M. McCabe Armando J. Mendez |
| author_facet | Angela Szeto Monia Cecati Raisa Ahmed Philip M. McCabe Armando J. Mendez |
| author_sort | Angela Szeto |
| collection | DOAJ |
| description | Abstract Background Obesity and adipose tissue expansion is characterized by a chronic state of systemic inflammation that contributes to disease. The neuropeptide, oxytocin, working through its receptor has been shown to attenuate inflammation in sepsis, wound healing, and cardiovascular disease. The current study examined the effects of chronic oxytocin infusions on adipose tissue inflammation in a murine model of obesity, the leptin receptor-deficient (db/db) mouse. Methods The effect of obesity on oxytocin receptor protein and mRNA expression in adipose tissue was evaluated by Western blotting and real-time polymerase chain reaction. Mice were implanted with osmotic minipumps filled with oxytocin or vehicle for 8 weeks. At study endpoint adipose tissue inflammation was assessed by measurement of cytokine and adipokine mRNA tissue levels, adipocyte size and macrophage infiltration via histopathology, and plasma levels of adiponectin and serum amyloid A as markers of systemic inflammation. Results The expression of adipose tissue oxytocin receptor was increased in obese db/db mice compared to lean controls. In adipose tissue oxytocin infusion reduced adipocyte size, macrophage infiltration, IL-6 and TNFα mRNA expression, and increased the expression of the anti-inflammatory adipokine, adiponectin. In plasma, oxytocin infusion reduced the level of serum amyloid A, a marker of systemic inflammation, and increased circulating adiponectin. Conclusions In an animal model of obesity and diabetes chronic oxytocin treatment led to a reduction in visceral adipose tissue inflammation and plasma markers of systemic inflammation, which may play a role in disease progression. |
| first_indexed | 2024-12-23T04:06:59Z |
| format | Article |
| id | doaj.art-e12d6bd5f4b443c08af6251b24507d80 |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-12-23T04:06:59Z |
| publishDate | 2020-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-e12d6bd5f4b443c08af6251b24507d802022-12-21T18:00:36ZengBMCLipids in Health and Disease1476-511X2020-08-0119111110.1186/s12944-020-01364-xOxytocin reduces adipose tissue inflammation in obese miceAngela Szeto0Monia Cecati1Raisa Ahmed2Philip M. McCabe3Armando J. Mendez4Department of Psychology, University of MiamiDepartment of Medicine and Diabetes Research Institute, University of Miami Miller School of MedicineDepartment of Psychology, University of MiamiDepartment of Psychology, University of MiamiDepartment of Medicine and Diabetes Research Institute, University of Miami Miller School of MedicineAbstract Background Obesity and adipose tissue expansion is characterized by a chronic state of systemic inflammation that contributes to disease. The neuropeptide, oxytocin, working through its receptor has been shown to attenuate inflammation in sepsis, wound healing, and cardiovascular disease. The current study examined the effects of chronic oxytocin infusions on adipose tissue inflammation in a murine model of obesity, the leptin receptor-deficient (db/db) mouse. Methods The effect of obesity on oxytocin receptor protein and mRNA expression in adipose tissue was evaluated by Western blotting and real-time polymerase chain reaction. Mice were implanted with osmotic minipumps filled with oxytocin or vehicle for 8 weeks. At study endpoint adipose tissue inflammation was assessed by measurement of cytokine and adipokine mRNA tissue levels, adipocyte size and macrophage infiltration via histopathology, and plasma levels of adiponectin and serum amyloid A as markers of systemic inflammation. Results The expression of adipose tissue oxytocin receptor was increased in obese db/db mice compared to lean controls. In adipose tissue oxytocin infusion reduced adipocyte size, macrophage infiltration, IL-6 and TNFα mRNA expression, and increased the expression of the anti-inflammatory adipokine, adiponectin. In plasma, oxytocin infusion reduced the level of serum amyloid A, a marker of systemic inflammation, and increased circulating adiponectin. Conclusions In an animal model of obesity and diabetes chronic oxytocin treatment led to a reduction in visceral adipose tissue inflammation and plasma markers of systemic inflammation, which may play a role in disease progression.http://link.springer.com/article/10.1186/s12944-020-01364-xAdipose tissueAnti-inflammationObesityOxytocinObese mouse model |
| spellingShingle | Angela Szeto Monia Cecati Raisa Ahmed Philip M. McCabe Armando J. Mendez Oxytocin reduces adipose tissue inflammation in obese mice Lipids in Health and Disease Adipose tissue Anti-inflammation Obesity Oxytocin Obese mouse model |
| title | Oxytocin reduces adipose tissue inflammation in obese mice |
| title_full | Oxytocin reduces adipose tissue inflammation in obese mice |
| title_fullStr | Oxytocin reduces adipose tissue inflammation in obese mice |
| title_full_unstemmed | Oxytocin reduces adipose tissue inflammation in obese mice |
| title_short | Oxytocin reduces adipose tissue inflammation in obese mice |
| title_sort | oxytocin reduces adipose tissue inflammation in obese mice |
| topic | Adipose tissue Anti-inflammation Obesity Oxytocin Obese mouse model |
| url | http://link.springer.com/article/10.1186/s12944-020-01364-x |
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