Establishment of an Indirect Competitive ELISA for Zearalenone
In this study, an indirect competitive ELISA (ic-ELISA) was developed for the detection of zearalenone in corn flour. The optimal working concentration of antigen and monoclonal antibody was determined by chessboard method. Antigen 37 ℃ coated for 1 h, unsealed and enzyme catalyzed substrate action...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
The editorial department of Science and Technology of Food Industry
2022-02-01
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| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021050177 |
| _version_ | 1828285849620447232 |
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| author | Song TANG Yansong LI Cuiling SHANG Pan HU Shiying LU Honglin REN Zengshan LIU Yu ZHOU |
| author_facet | Song TANG Yansong LI Cuiling SHANG Pan HU Shiying LU Honglin REN Zengshan LIU Yu ZHOU |
| author_sort | Song TANG |
| collection | DOAJ |
| description | In this study, an indirect competitive ELISA (ic-ELISA) was developed for the detection of zearalenone in corn flour. The optimal working concentration of antigen and monoclonal antibody was determined by chessboard method. Antigen 37 ℃ coated for 1 h, unsealed and enzyme catalyzed substrate action time of 15 min were determined the optimal conditions. The results showed that the detection range (IC20~IC80) was 11~292 pg/mL, and the minimum detection limit (IC10) was 6 pg/mL. The recoveries of the zearalenone from corn flour were 81.29%~105.80%. There was no cross reaction with ochratoxin A, aflatoxin B1, deoxynivalenol, fumonisin B1, T-2 toxin. The ic-ELISA could be used for detection of zearalenon in corn flour. |
| first_indexed | 2024-04-13T09:21:36Z |
| format | Article |
| id | doaj.art-e1342eebb6234308a9c2319172ec80db |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-13T09:21:36Z |
| publishDate | 2022-02-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-e1342eebb6234308a9c2319172ec80db2022-12-22T02:52:35ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-02-0143430030410.13386/j.issn1002-0306.20210501772021050177-4Establishment of an Indirect Competitive ELISA for ZearalenoneSong TANG0Yansong LI1Cuiling SHANG2Pan HU3Shiying LU4Honglin REN5Zengshan LIU6Yu ZHOU7Institute of Zoonosis, Jilin University, Changchun 130062, ChinaInstitute of Zoonosis, Jilin University, Changchun 130062, ChinaInstitute of Zoonosis, Jilin University, Changchun 130062, ChinaInstitute of Zoonosis, Jilin University, Changchun 130062, ChinaInstitute of Zoonosis, Jilin University, Changchun 130062, ChinaInstitute of Zoonosis, Jilin University, Changchun 130062, ChinaInstitute of Zoonosis, Jilin University, Changchun 130062, ChinaInstitute of Zoonosis, Jilin University, Changchun 130062, ChinaIn this study, an indirect competitive ELISA (ic-ELISA) was developed for the detection of zearalenone in corn flour. The optimal working concentration of antigen and monoclonal antibody was determined by chessboard method. Antigen 37 ℃ coated for 1 h, unsealed and enzyme catalyzed substrate action time of 15 min were determined the optimal conditions. The results showed that the detection range (IC20~IC80) was 11~292 pg/mL, and the minimum detection limit (IC10) was 6 pg/mL. The recoveries of the zearalenone from corn flour were 81.29%~105.80%. There was no cross reaction with ochratoxin A, aflatoxin B1, deoxynivalenol, fumonisin B1, T-2 toxin. The ic-ELISA could be used for detection of zearalenon in corn flour.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021050177zearalenonetoxindetectionindirect competitive enzyme linked immunosorbent assay (ic-elisa) |
| spellingShingle | Song TANG Yansong LI Cuiling SHANG Pan HU Shiying LU Honglin REN Zengshan LIU Yu ZHOU Establishment of an Indirect Competitive ELISA for Zearalenone Shipin gongye ke-ji zearalenone toxin detection indirect competitive enzyme linked immunosorbent assay (ic-elisa) |
| title | Establishment of an Indirect Competitive ELISA for Zearalenone |
| title_full | Establishment of an Indirect Competitive ELISA for Zearalenone |
| title_fullStr | Establishment of an Indirect Competitive ELISA for Zearalenone |
| title_full_unstemmed | Establishment of an Indirect Competitive ELISA for Zearalenone |
| title_short | Establishment of an Indirect Competitive ELISA for Zearalenone |
| title_sort | establishment of an indirect competitive elisa for zearalenone |
| topic | zearalenone toxin detection indirect competitive enzyme linked immunosorbent assay (ic-elisa) |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021050177 |
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