Design and Study of Nanoceria Modified by 5-Fluorouracil for Gel and Polymer Dermal Film Preparation
In this work we studied nanoceria (CeO<sub>2</sub>NPs) and nanoceria modified by 5-fluorouracil (5FU) as potential APIs. Nanoceria were synthesized by precipitation in a matrix of hydroxyethyl cellulose or hydroxypropylmethyl cellulose, using cerium (III) nitrate and meglumine. Nanoceria...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/16/8/1082 |
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| author | Nina Melnikova Ilya Sheferov Dmitry Panteleev Anastasia Emasheva Irina Druzhkova Nadezhda Ignatova Tatiana Mishchenko Maria Vedunova |
| author_facet | Nina Melnikova Ilya Sheferov Dmitry Panteleev Anastasia Emasheva Irina Druzhkova Nadezhda Ignatova Tatiana Mishchenko Maria Vedunova |
| author_sort | Nina Melnikova |
| collection | DOAJ |
| description | In this work we studied nanoceria (CeO<sub>2</sub>NPs) and nanoceria modified by 5-fluorouracil (5FU) as potential APIs. Nanoceria were synthesized by precipitation in a matrix of hydroxyethyl cellulose or hydroxypropylmethyl cellulose, using cerium (III) nitrate and meglumine. Nanoceria properties were estimated by UV, FTIR and X-ray photoelectron spectra; scanning electron and atomic force microscopy; powder X-ray diffraction patterns and energy dispersive X-ray microanalysis. The cytotoxicity of nanoceria and polymer-protected nanoparticles was evaluated using the established cell line NCTC clone 929 (C3H/An mouse, subcutaneous connective tissue, clone of L. line). The morphology and metabolic activity of nanoparticles at 10 μg∙mL<sup>−1</sup> of cells was not significant. In addition, the cytotoxic effects of nanoceria were assessed on two human colorectal cancer cell lines (HT29 and HCT116), murine melanoma B16 cells and normal human skin fibroblasts. An inhibitory effect was shown for HCT116 human colorectal cancer cells. The IC<sub>50</sub> values for pure CeO<sub>2</sub>NPs and CeO<sub>2</sub>NPs-5FU were 219.0 ± 45.6 μg∙mL<sup>−1</sup> and 89.2 ± 14.0 μg∙mL<sup>−1</sup>, respectively. On the other hand, the IC<sub>50</sub> of 5FU in the combination of CeO<sub>2</sub>NPs-5FU was 2-fold higher than that of pure 5FU, amounting to 5.0 nmol∙mL<sup>−1</sup>. New compositions of nanoceria modified by 5-fluorouracil in a polymer matrix were designed as a dermal polymer film and gel. The permeability of the components was studied using a Franz cell. |
| first_indexed | 2024-03-10T23:40:01Z |
| format | Article |
| id | doaj.art-e13c98f161fc411ebc8091c6965e4b63 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T23:40:01Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-e13c98f161fc411ebc8091c6965e4b632023-11-19T02:33:37ZengMDPI AGPharmaceuticals1424-82472023-07-01168108210.3390/ph16081082Design and Study of Nanoceria Modified by 5-Fluorouracil for Gel and Polymer Dermal Film PreparationNina Melnikova0Ilya Sheferov1Dmitry Panteleev2Anastasia Emasheva3Irina Druzhkova4Nadezhda Ignatova5Tatiana Mishchenko6Maria Vedunova7Faculty of Chemistry, Lobachevsky State University, 23 Gagarin Av., 603022 Nizhny Novgorod, RussiaFaculty of Chemistry, Lobachevsky State University, 23 Gagarin Av., 603022 Nizhny Novgorod, RussiaDepartment of Pharmaceutical Chemistry, Privolzhsky Research Medical University, 10/1 Minin Sq., 603950 Nizhny Novgorod, RussiaFaculty of Chemistry, Lobachevsky State University, 23 Gagarin Av., 603022 Nizhny Novgorod, RussiaResearch Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin Sq., 603950 Nizhny Novgorod, RussiaResearch Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin Sq., 603950 Nizhny Novgorod, RussiaInstitute of Biology and Biomedicine, Lobachevsky State University, 23 Gagarin Av., 603022 Nizhny Novgorod, RussiaInstitute of Biology and Biomedicine, Lobachevsky State University, 23 Gagarin Av., 603022 Nizhny Novgorod, RussiaIn this work we studied nanoceria (CeO<sub>2</sub>NPs) and nanoceria modified by 5-fluorouracil (5FU) as potential APIs. Nanoceria were synthesized by precipitation in a matrix of hydroxyethyl cellulose or hydroxypropylmethyl cellulose, using cerium (III) nitrate and meglumine. Nanoceria properties were estimated by UV, FTIR and X-ray photoelectron spectra; scanning electron and atomic force microscopy; powder X-ray diffraction patterns and energy dispersive X-ray microanalysis. The cytotoxicity of nanoceria and polymer-protected nanoparticles was evaluated using the established cell line NCTC clone 929 (C3H/An mouse, subcutaneous connective tissue, clone of L. line). The morphology and metabolic activity of nanoparticles at 10 μg∙mL<sup>−1</sup> of cells was not significant. In addition, the cytotoxic effects of nanoceria were assessed on two human colorectal cancer cell lines (HT29 and HCT116), murine melanoma B16 cells and normal human skin fibroblasts. An inhibitory effect was shown for HCT116 human colorectal cancer cells. The IC<sub>50</sub> values for pure CeO<sub>2</sub>NPs and CeO<sub>2</sub>NPs-5FU were 219.0 ± 45.6 μg∙mL<sup>−1</sup> and 89.2 ± 14.0 μg∙mL<sup>−1</sup>, respectively. On the other hand, the IC<sub>50</sub> of 5FU in the combination of CeO<sub>2</sub>NPs-5FU was 2-fold higher than that of pure 5FU, amounting to 5.0 nmol∙mL<sup>−1</sup>. New compositions of nanoceria modified by 5-fluorouracil in a polymer matrix were designed as a dermal polymer film and gel. The permeability of the components was studied using a Franz cell.https://www.mdpi.com/1424-8247/16/8/1082nanoceria modified by 5FUpolymer dermal filmshydrogelcytotoxicity |
| spellingShingle | Nina Melnikova Ilya Sheferov Dmitry Panteleev Anastasia Emasheva Irina Druzhkova Nadezhda Ignatova Tatiana Mishchenko Maria Vedunova Design and Study of Nanoceria Modified by 5-Fluorouracil for Gel and Polymer Dermal Film Preparation Pharmaceuticals nanoceria modified by 5FU polymer dermal films hydrogel cytotoxicity |
| title | Design and Study of Nanoceria Modified by 5-Fluorouracil for Gel and Polymer Dermal Film Preparation |
| title_full | Design and Study of Nanoceria Modified by 5-Fluorouracil for Gel and Polymer Dermal Film Preparation |
| title_fullStr | Design and Study of Nanoceria Modified by 5-Fluorouracil for Gel and Polymer Dermal Film Preparation |
| title_full_unstemmed | Design and Study of Nanoceria Modified by 5-Fluorouracil for Gel and Polymer Dermal Film Preparation |
| title_short | Design and Study of Nanoceria Modified by 5-Fluorouracil for Gel and Polymer Dermal Film Preparation |
| title_sort | design and study of nanoceria modified by 5 fluorouracil for gel and polymer dermal film preparation |
| topic | nanoceria modified by 5FU polymer dermal films hydrogel cytotoxicity |
| url | https://www.mdpi.com/1424-8247/16/8/1082 |
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