Silk Fibroin Films Facilitate Single-Step Targeted Expression of Optogenetic Proteins
Summary: Optical methods of interrogating neural circuits have emerged as powerful tools for understanding how the brain drives behaviors. Optogenetic proteins are widely used to control neuronal activity, while genetically encoded fluorescent reporters are used to monitor activity. These proteins a...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018-03-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124718302912 |
| _version_ | 1828518639021588480 |
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| author | Skyler L. Jackman Christopher H. Chen Selmaan N. Chettih Shay Q. Neufeld Iain R. Drew Chimuanya K. Agba Isabella Flaquer Alexis N. Stefano Thomas J. Kennedy Justine E. Belinsky Keiramarie Roberston Celia C. Beron Bernardo L. Sabatini Christopher D. Harvey Wade G. Regehr |
| author_facet | Skyler L. Jackman Christopher H. Chen Selmaan N. Chettih Shay Q. Neufeld Iain R. Drew Chimuanya K. Agba Isabella Flaquer Alexis N. Stefano Thomas J. Kennedy Justine E. Belinsky Keiramarie Roberston Celia C. Beron Bernardo L. Sabatini Christopher D. Harvey Wade G. Regehr |
| author_sort | Skyler L. Jackman |
| collection | DOAJ |
| description | Summary: Optical methods of interrogating neural circuits have emerged as powerful tools for understanding how the brain drives behaviors. Optogenetic proteins are widely used to control neuronal activity, while genetically encoded fluorescent reporters are used to monitor activity. These proteins are often expressed by injecting viruses, which frequently leads to inconsistent experiments due to misalignment of expression and optical components. Here, we describe how silk fibroin films simplify optogenetic experiments by providing targeted delivery of viruses. Films composed of silk fibroin and virus are applied to the surface of implantable optical components. After surgery, silk releases the virus to transduce nearby cells and provide localized expression around optical fibers and endoscopes. Silk films can also be used to express genetically encoded sensors in large cortical regions by using cranial windows coated with a silk/virus mixture. The ease of use and improved performance provided by silk make this a promising approach for optogenetic studies. : Jackman et al. show that coating optical implants with silk fibroin mixed with AAV allows single-step implantation and expression of optogenetic proteins like channelrhodopsin and GCaMP. Keywords: silk, optogenetics, optical fiber implants, cranial windows, in vivo imaging, 2-photon calcium imaging, tapered optical fibers, biomaterials, viral vectors, stereotaxic injections |
| first_indexed | 2024-12-11T18:58:18Z |
| format | Article |
| id | doaj.art-e13ca224def64c05abf9118f1cf56701 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-11T18:58:18Z |
| publishDate | 2018-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-e13ca224def64c05abf9118f1cf567012022-12-22T00:54:05ZengElsevierCell Reports2211-12472018-03-01221233513361Silk Fibroin Films Facilitate Single-Step Targeted Expression of Optogenetic ProteinsSkyler L. Jackman0Christopher H. Chen1Selmaan N. Chettih2Shay Q. Neufeld3Iain R. Drew4Chimuanya K. Agba5Isabella Flaquer6Alexis N. Stefano7Thomas J. Kennedy8Justine E. Belinsky9Keiramarie Roberston10Celia C. Beron11Bernardo L. Sabatini12Christopher D. Harvey13Wade G. Regehr14Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USADepartment of Neurobiology, Harvard Medical School, Boston, MA 02115, USADepartment of Neurobiology, Harvard Medical School, Boston, MA 02115, USADepartment of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USADepartment of Neurobiology, Harvard Medical School, Boston, MA 02115, USADepartment of Neurobiology, Harvard Medical School, Boston, MA 02115, USADepartment of Neurobiology, Harvard Medical School, Boston, MA 02115, USADepartment of Neurobiology, Harvard Medical School, Boston, MA 02115, USADepartment of Neurobiology, Harvard Medical School, Boston, MA 02115, USADepartment of Neurobiology, Harvard Medical School, Boston, MA 02115, USADepartment of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USADepartment of Neurobiology, Harvard Medical School, Boston, MA 02115, USADepartment of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USADepartment of Neurobiology, Harvard Medical School, Boston, MA 02115, USADepartment of Neurobiology, Harvard Medical School, Boston, MA 02115, USA; Corresponding authorSummary: Optical methods of interrogating neural circuits have emerged as powerful tools for understanding how the brain drives behaviors. Optogenetic proteins are widely used to control neuronal activity, while genetically encoded fluorescent reporters are used to monitor activity. These proteins are often expressed by injecting viruses, which frequently leads to inconsistent experiments due to misalignment of expression and optical components. Here, we describe how silk fibroin films simplify optogenetic experiments by providing targeted delivery of viruses. Films composed of silk fibroin and virus are applied to the surface of implantable optical components. After surgery, silk releases the virus to transduce nearby cells and provide localized expression around optical fibers and endoscopes. Silk films can also be used to express genetically encoded sensors in large cortical regions by using cranial windows coated with a silk/virus mixture. The ease of use and improved performance provided by silk make this a promising approach for optogenetic studies. : Jackman et al. show that coating optical implants with silk fibroin mixed with AAV allows single-step implantation and expression of optogenetic proteins like channelrhodopsin and GCaMP. Keywords: silk, optogenetics, optical fiber implants, cranial windows, in vivo imaging, 2-photon calcium imaging, tapered optical fibers, biomaterials, viral vectors, stereotaxic injectionshttp://www.sciencedirect.com/science/article/pii/S2211124718302912 |
| spellingShingle | Skyler L. Jackman Christopher H. Chen Selmaan N. Chettih Shay Q. Neufeld Iain R. Drew Chimuanya K. Agba Isabella Flaquer Alexis N. Stefano Thomas J. Kennedy Justine E. Belinsky Keiramarie Roberston Celia C. Beron Bernardo L. Sabatini Christopher D. Harvey Wade G. Regehr Silk Fibroin Films Facilitate Single-Step Targeted Expression of Optogenetic Proteins Cell Reports |
| title | Silk Fibroin Films Facilitate Single-Step Targeted Expression of Optogenetic Proteins |
| title_full | Silk Fibroin Films Facilitate Single-Step Targeted Expression of Optogenetic Proteins |
| title_fullStr | Silk Fibroin Films Facilitate Single-Step Targeted Expression of Optogenetic Proteins |
| title_full_unstemmed | Silk Fibroin Films Facilitate Single-Step Targeted Expression of Optogenetic Proteins |
| title_short | Silk Fibroin Films Facilitate Single-Step Targeted Expression of Optogenetic Proteins |
| title_sort | silk fibroin films facilitate single step targeted expression of optogenetic proteins |
| url | http://www.sciencedirect.com/science/article/pii/S2211124718302912 |
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