Apigenin reduces the suppressive effect of exosomes derived from irritable bowel syndrome patients on the autophagy of human colon epithelial cells by promoting ATG14
Abstract Background Inflammatory bowel disease (IBS) is a chronic disorder of the gastrointestinal tract. Exosomes have been involved in various pathological processes including IBS. Apigenin has been reported to suppress inflammatory bowel disease (IBS). However, the regulatory roles of exosomes de...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-03-01
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| Series: | World Journal of Surgical Oncology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12957-023-02963-5 |
| _version_ | 1827983955276595200 |
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| author | Rui Fu Saiyue Liu Mingjin Zhu Jiajie Zhu Mingxian Chen |
| author_facet | Rui Fu Saiyue Liu Mingjin Zhu Jiajie Zhu Mingxian Chen |
| author_sort | Rui Fu |
| collection | DOAJ |
| description | Abstract Background Inflammatory bowel disease (IBS) is a chronic disorder of the gastrointestinal tract. Exosomes have been involved in various pathological processes including IBS. Apigenin has been reported to suppress inflammatory bowel disease (IBS). However, the regulatory roles of exosomes derived from IBS patients (IBS-exos) on human colon epithelial cells are still unclear. Methods Exosomes were collected from IBS patients (IBS-exos) and co-cultured with CACO-2 cells. Apigenin was used to treat IBS-exos-treated CACO-2 cells. By exploring the public data bank, we figured out the regulators control the autophagy of CACO-2 cells. Results Administration of apigenin dose-dependently abolished the inhibitory effect of IBS-exo on the autophagy of CACO-2 cells. A mechanistic study showed that miR-148b-3p bound to 3′UTR to suppress ATG14 and decrease autophagy. Moreover, results suggested that ATG14 overexpression promoted the autophagy of CACO-2 cells in the presence of miR-148b-3p mimic. Conclusion The current study showed that apigenin dose-dependently abolished the inhibitory effect of IBS-exo on CACO-2 cell autophagy by regulating miR-148b-3p/ATG14 signaling. |
| first_indexed | 2024-04-09T22:54:24Z |
| format | Article |
| id | doaj.art-e13cdf5657d147f6a4da9767565e30c4 |
| institution | Directory Open Access Journal |
| issn | 1477-7819 |
| language | English |
| last_indexed | 2024-04-09T22:54:24Z |
| publishDate | 2023-03-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj.art-e13cdf5657d147f6a4da9767565e30c42023-03-22T11:24:23ZengBMCWorld Journal of Surgical Oncology1477-78192023-03-012111910.1186/s12957-023-02963-5Apigenin reduces the suppressive effect of exosomes derived from irritable bowel syndrome patients on the autophagy of human colon epithelial cells by promoting ATG14Rui Fu0Saiyue Liu1Mingjin Zhu2Jiajie Zhu3Mingxian Chen4Department of Gastroenterology, Tongde Hospital of Zhejiang ProvinceDepartment of Gastroenterology, Tongde Hospital of Zhejiang ProvinceDepartment of Adverse Drug Reaction Monitoring, Zhejiang Province Center of Adverse Drug Reaction MonitoringDepartment of Gastroenterology, Tongde Hospital of Zhejiang ProvinceDepartment of Gastroenterology, Tongde Hospital of Zhejiang ProvinceAbstract Background Inflammatory bowel disease (IBS) is a chronic disorder of the gastrointestinal tract. Exosomes have been involved in various pathological processes including IBS. Apigenin has been reported to suppress inflammatory bowel disease (IBS). However, the regulatory roles of exosomes derived from IBS patients (IBS-exos) on human colon epithelial cells are still unclear. Methods Exosomes were collected from IBS patients (IBS-exos) and co-cultured with CACO-2 cells. Apigenin was used to treat IBS-exos-treated CACO-2 cells. By exploring the public data bank, we figured out the regulators control the autophagy of CACO-2 cells. Results Administration of apigenin dose-dependently abolished the inhibitory effect of IBS-exo on the autophagy of CACO-2 cells. A mechanistic study showed that miR-148b-3p bound to 3′UTR to suppress ATG14 and decrease autophagy. Moreover, results suggested that ATG14 overexpression promoted the autophagy of CACO-2 cells in the presence of miR-148b-3p mimic. Conclusion The current study showed that apigenin dose-dependently abolished the inhibitory effect of IBS-exo on CACO-2 cell autophagy by regulating miR-148b-3p/ATG14 signaling.https://doi.org/10.1186/s12957-023-02963-5ApigeninExosomeIrritable bowel syndromeAutophagyMicroRNA |
| spellingShingle | Rui Fu Saiyue Liu Mingjin Zhu Jiajie Zhu Mingxian Chen Apigenin reduces the suppressive effect of exosomes derived from irritable bowel syndrome patients on the autophagy of human colon epithelial cells by promoting ATG14 World Journal of Surgical Oncology Apigenin Exosome Irritable bowel syndrome Autophagy MicroRNA |
| title | Apigenin reduces the suppressive effect of exosomes derived from irritable bowel syndrome patients on the autophagy of human colon epithelial cells by promoting ATG14 |
| title_full | Apigenin reduces the suppressive effect of exosomes derived from irritable bowel syndrome patients on the autophagy of human colon epithelial cells by promoting ATG14 |
| title_fullStr | Apigenin reduces the suppressive effect of exosomes derived from irritable bowel syndrome patients on the autophagy of human colon epithelial cells by promoting ATG14 |
| title_full_unstemmed | Apigenin reduces the suppressive effect of exosomes derived from irritable bowel syndrome patients on the autophagy of human colon epithelial cells by promoting ATG14 |
| title_short | Apigenin reduces the suppressive effect of exosomes derived from irritable bowel syndrome patients on the autophagy of human colon epithelial cells by promoting ATG14 |
| title_sort | apigenin reduces the suppressive effect of exosomes derived from irritable bowel syndrome patients on the autophagy of human colon epithelial cells by promoting atg14 |
| topic | Apigenin Exosome Irritable bowel syndrome Autophagy MicroRNA |
| url | https://doi.org/10.1186/s12957-023-02963-5 |
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