A topical ointment formulation containing leaves' powder of Lawsonia inermis accelerate excision wound healing in Wistar rats

Aim: Lawsonia inermis (LI), a naturally grown or cultivated shrub in Northeast of Africa and India, has been traditionally used as a strong remedy for several injuries. However, few studies have reported its use as a cicatrizing agent. The aim of this study was to evaluate the effect of daily applic...

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Main Authors: Kalbaza Ahmed Yassine, Hemida Houari, Benchohra Mokhtar, Amara Karim, Salem Hadjer, Bediaf Imane
Format: Article
Language:English
Published: Veterinary World 2020-07-01
Series:Veterinary World
Subjects:
Online Access:http://www.veterinaryworld.org/Vol.13/July-2020/5.pdf
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author Kalbaza Ahmed Yassine
Hemida Houari
Benchohra Mokhtar
Amara Karim
Salem Hadjer
Bediaf Imane
author_facet Kalbaza Ahmed Yassine
Hemida Houari
Benchohra Mokhtar
Amara Karim
Salem Hadjer
Bediaf Imane
author_sort Kalbaza Ahmed Yassine
collection DOAJ
description Aim: Lawsonia inermis (LI), a naturally grown or cultivated shrub in Northeast of Africa and India, has been traditionally used as a strong remedy for several injuries. However, few studies have reported its use as a cicatrizing agent. The aim of this study was to evaluate the effect of daily application of an ointment prepared with LI leaves' powder on wound healing in Wistar rats. Materials and Methods: Twenty female Wistar rats were used in this study. Excisional wound model was realized by removing skin from the dorsal part of the neck of each animal. Wounds have been then treated by a daily application of LI ointment prepared by mixing leaves' powder to petroleum jelly in test group and by simple application of petroleum jelly in control group. Evaluation of wound healing activity was then based on calculating the percentage of wound contraction, period of epithelialization, and wound index every 3 days for a period of 24 days, then, a histological study of the healed excised wound was performed. Results: Treatment with LI has shown excellent wound healing activity, since it has increased percent of wound contraction, and reduced period of epithelialization and wound index as compared to control (p<0.05). These results have been supported by the histological findings that revealed better epithelialization, dermal differentiation, collagen fiber orientation, and angiogenesis in LI treated rats compared to control (p<0.05). Conclusion: We can conclude that LI leaves' can be used as a potential wound healing agent.
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spelling doaj.art-e13d61f91e0149f4a5626dfeee9c1f1d2022-12-21T18:32:33ZengVeterinary WorldVeterinary World0972-89882231-09162020-07-011371280128710.14202/vetworld.2020.1280-1287A topical ointment formulation containing leaves' powder of Lawsonia inermis accelerate excision wound healing in Wistar ratsKalbaza Ahmed Yassine0Hemida Houari1Benchohra Mokhtar2Amara Karim3Salem Hadjer4Bediaf Imane5Department of Veterinary Sciences, Institute of Veterinary and Agronomic Sciences, University of BATNA-1, Algeria; Laboratory of Agro-Biotechnology and Nutrition in Semi-Arid Regions, University of Tiaret, Algeria.Laboratory of Agro-Biotechnology and Nutrition in Semi-Arid Regions, University of Tiaret, Algeria; Department of Animal Health, Institute of Veterinary Sciences, University of Tiaret, Algeria.Laboratory of Agro-Biotechnology and Nutrition in Semi-Arid Regions, University of Tiaret, Algeria; Department of Animal Health, Institute of Veterinary Sciences, University of Tiaret, Algeria.Laboratory of Agro-Biotechnology and Nutrition in Semi-Arid Regions, University of Tiaret, Algeria; Department of Animal Health, Institute of Veterinary Sciences, University of Tiaret, Algeria.Department of Veterinary Sciences, Institute of Veterinary and Agronomic Sciences, University of BATNA-1, Algeria.Department of Veterinary Sciences, Institute of Veterinary and Agronomic Sciences, University of BATNA-1, Algeria.Aim: Lawsonia inermis (LI), a naturally grown or cultivated shrub in Northeast of Africa and India, has been traditionally used as a strong remedy for several injuries. However, few studies have reported its use as a cicatrizing agent. The aim of this study was to evaluate the effect of daily application of an ointment prepared with LI leaves' powder on wound healing in Wistar rats. Materials and Methods: Twenty female Wistar rats were used in this study. Excisional wound model was realized by removing skin from the dorsal part of the neck of each animal. Wounds have been then treated by a daily application of LI ointment prepared by mixing leaves' powder to petroleum jelly in test group and by simple application of petroleum jelly in control group. Evaluation of wound healing activity was then based on calculating the percentage of wound contraction, period of epithelialization, and wound index every 3 days for a period of 24 days, then, a histological study of the healed excised wound was performed. Results: Treatment with LI has shown excellent wound healing activity, since it has increased percent of wound contraction, and reduced period of epithelialization and wound index as compared to control (p<0.05). These results have been supported by the histological findings that revealed better epithelialization, dermal differentiation, collagen fiber orientation, and angiogenesis in LI treated rats compared to control (p<0.05). Conclusion: We can conclude that LI leaves' can be used as a potential wound healing agent.http://www.veterinaryworld.org/Vol.13/July-2020/5.pdfexcisionlawsonia inermispetroleum jellywistar ratswound healing
spellingShingle Kalbaza Ahmed Yassine
Hemida Houari
Benchohra Mokhtar
Amara Karim
Salem Hadjer
Bediaf Imane
A topical ointment formulation containing leaves' powder of Lawsonia inermis accelerate excision wound healing in Wistar rats
Veterinary World
excision
lawsonia inermis
petroleum jelly
wistar rats
wound healing
title A topical ointment formulation containing leaves' powder of Lawsonia inermis accelerate excision wound healing in Wistar rats
title_full A topical ointment formulation containing leaves' powder of Lawsonia inermis accelerate excision wound healing in Wistar rats
title_fullStr A topical ointment formulation containing leaves' powder of Lawsonia inermis accelerate excision wound healing in Wistar rats
title_full_unstemmed A topical ointment formulation containing leaves' powder of Lawsonia inermis accelerate excision wound healing in Wistar rats
title_short A topical ointment formulation containing leaves' powder of Lawsonia inermis accelerate excision wound healing in Wistar rats
title_sort topical ointment formulation containing leaves powder of lawsonia inermis accelerate excision wound healing in wistar rats
topic excision
lawsonia inermis
petroleum jelly
wistar rats
wound healing
url http://www.veterinaryworld.org/Vol.13/July-2020/5.pdf
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