Distribution of Hydrogen-Producing Bacteria in Tibetan Hot Springs, China
Investigating the distribution of hydrogen-producing bacteria (HPB) is of great significance to understanding the source of biological hydrogen production in geothermal environments. Here, we explored the compositions of HPB populations in the sediments of hot springs from the Daggyai, Quzhuomu, Qus...
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.569020/full |
| _version_ | 1818355637811150848 |
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| author | Li Ma Geng Wu Jian Yang Liuqin Huang Dorji Phurbu Wen-Jun Li Hongchen Jiang |
| author_facet | Li Ma Geng Wu Jian Yang Liuqin Huang Dorji Phurbu Wen-Jun Li Hongchen Jiang |
| author_sort | Li Ma |
| collection | DOAJ |
| description | Investigating the distribution of hydrogen-producing bacteria (HPB) is of great significance to understanding the source of biological hydrogen production in geothermal environments. Here, we explored the compositions of HPB populations in the sediments of hot springs from the Daggyai, Quzhuomu, Quseyongba, and Moluojiang geothermal zones on the Tibetan Plateau, with the use of Illumina MiSeq high-throughput sequencing of 16S rRNA genes and hydA genes. In the present study, the hydA genes were successfully amplified from the hot springs with a temperature of 46–87°C. The hydA gene phylogenetic analysis showed that the top three phyla of the HPB populations were Bacteroidetes (14.48%), Spirochaetes (14.12%), and Thermotogae (10.45%), while Proteobacteria were absent in the top 10 of the HPB populations, although Proteobacteria were dominant in the 16S rRNA gene sequences. Canonical correspondence analysis results indicate that the HPB community structure in the studied Tibetan hot springs was correlated with various environmental factors, such as temperature, pH, and elevation. The HPB community structure also showed a spatial distribution pattern; samples from the same area showed similar community structures. Furthermore, one HPB isolate affiliated with Firmicutes was obtained and demonstrated the capacity of hydrogen production. These results are important for us to understand the distribution and function of HPB in hot springs. |
| first_indexed | 2024-12-13T19:44:29Z |
| format | Article |
| id | doaj.art-e13df678820e42948a360d261e7f2375 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T19:44:29Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-e13df678820e42948a360d261e7f23752022-12-21T23:33:35ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-07-011210.3389/fmicb.2021.569020569020Distribution of Hydrogen-Producing Bacteria in Tibetan Hot Springs, ChinaLi Ma0Geng Wu1Jian Yang2Liuqin Huang3Dorji Phurbu4Wen-Jun Li5Hongchen Jiang6State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, ChinaState Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, ChinaState Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, ChinaState Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, ChinaTibet Plateau Institute of Biology, Lhasa, ChinaState Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou, ChinaState Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, ChinaInvestigating the distribution of hydrogen-producing bacteria (HPB) is of great significance to understanding the source of biological hydrogen production in geothermal environments. Here, we explored the compositions of HPB populations in the sediments of hot springs from the Daggyai, Quzhuomu, Quseyongba, and Moluojiang geothermal zones on the Tibetan Plateau, with the use of Illumina MiSeq high-throughput sequencing of 16S rRNA genes and hydA genes. In the present study, the hydA genes were successfully amplified from the hot springs with a temperature of 46–87°C. The hydA gene phylogenetic analysis showed that the top three phyla of the HPB populations were Bacteroidetes (14.48%), Spirochaetes (14.12%), and Thermotogae (10.45%), while Proteobacteria were absent in the top 10 of the HPB populations, although Proteobacteria were dominant in the 16S rRNA gene sequences. Canonical correspondence analysis results indicate that the HPB community structure in the studied Tibetan hot springs was correlated with various environmental factors, such as temperature, pH, and elevation. The HPB community structure also showed a spatial distribution pattern; samples from the same area showed similar community structures. Furthermore, one HPB isolate affiliated with Firmicutes was obtained and demonstrated the capacity of hydrogen production. These results are important for us to understand the distribution and function of HPB in hot springs.https://www.frontiersin.org/articles/10.3389/fmicb.2021.569020/fullTibethot springshydrogen-producing bacteria[FeFe]-hydrogenasetemperature |
| spellingShingle | Li Ma Geng Wu Jian Yang Liuqin Huang Dorji Phurbu Wen-Jun Li Hongchen Jiang Distribution of Hydrogen-Producing Bacteria in Tibetan Hot Springs, China Frontiers in Microbiology Tibet hot springs hydrogen-producing bacteria [FeFe]-hydrogenase temperature |
| title | Distribution of Hydrogen-Producing Bacteria in Tibetan Hot Springs, China |
| title_full | Distribution of Hydrogen-Producing Bacteria in Tibetan Hot Springs, China |
| title_fullStr | Distribution of Hydrogen-Producing Bacteria in Tibetan Hot Springs, China |
| title_full_unstemmed | Distribution of Hydrogen-Producing Bacteria in Tibetan Hot Springs, China |
| title_short | Distribution of Hydrogen-Producing Bacteria in Tibetan Hot Springs, China |
| title_sort | distribution of hydrogen producing bacteria in tibetan hot springs china |
| topic | Tibet hot springs hydrogen-producing bacteria [FeFe]-hydrogenase temperature |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.569020/full |
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