Potato Peels Mediated Synthesis of Cu(II)-nanoparticles from Tyrosinase Reacted with bis-(<i>N</i>-aminoethylethanolamine) (Tyr-Cu(II)-AEEA NPs) and Their Cytotoxicity against Michigan Cancer Foundation-7 Breast Cancer Cell Line
The synthesis of nanoparticles is most important in the context of cancer therapy, particularly copper nanoparticles, which are widely used. In this work, copper(II)-tyrosinase was isolated from potato peel powder. Copper nanoparticles (Tyr-Cu(II)-AEEA NPs) were synthesized via the reaction of tyros...
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2021-11-01
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author | Akbar Idhayadhulla Aseer Manilal Anis Ahamed Saud Alarifi Gurusamy Raman |
author_facet | Akbar Idhayadhulla Aseer Manilal Anis Ahamed Saud Alarifi Gurusamy Raman |
author_sort | Akbar Idhayadhulla |
collection | DOAJ |
description | The synthesis of nanoparticles is most important in the context of cancer therapy, particularly copper nanoparticles, which are widely used. In this work, copper(II)-tyrosinase was isolated from potato peel powder. Copper nanoparticles (Tyr-Cu(II)-AEEA NPs) were synthesized via the reaction of tyrosinase with <i>N</i>-aminoethylethanolamine to produce Cu(II)-NPs and these were characterized by means of FT-IR, UV-Spectroscopy, XRD, SEM, TEM and a particle size analyzer. These Tyr-Cu(II)-AEEA NPs were tested as anticancer agents against MCF-7 breast cancer cells. Fluorescence microscopy and DNA fragmentation were also performed, which revealed the inhibiting potentials of Cu(II)-AEEA NPs and consequent cell death; Tyr-Cu(II)-AEEA NPs show potential cytotoxicity activity and this nano material could be contemplated as an anticancer medicament in future investigations. |
first_indexed | 2024-03-10T05:56:16Z |
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language | English |
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publishDate | 2021-11-01 |
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series | Molecules |
spelling | doaj.art-e13e947188624a3591aa01c0d2855a212023-11-22T21:24:41ZengMDPI AGMolecules1420-30492021-11-012621666510.3390/molecules26216665Potato Peels Mediated Synthesis of Cu(II)-nanoparticles from Tyrosinase Reacted with bis-(<i>N</i>-aminoethylethanolamine) (Tyr-Cu(II)-AEEA NPs) and Their Cytotoxicity against Michigan Cancer Foundation-7 Breast Cancer Cell LineAkbar Idhayadhulla0Aseer Manilal1Anis Ahamed2Saud Alarifi3Gurusamy Raman4Research Department of Chemistry, Nehru Memorial College, Affiliated Bharathidasan University, Puthanamapatti 621007, Tamil Nadu, IndiaDepartment of Medical Laboratory Science, College of Medicine and Health Sciences, Arba Minch University, Arba Minch P.O. Box No. 21, EthiopiaDepartment of Botany & Microbiology, College of Sciences, King Saud University (KSU), P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Zoology, College of Sciences, King Saud University (KSU), P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Life Science, Yeungnam University, Gyeongsan 38541, Gyeongbuk-do, KoreaThe synthesis of nanoparticles is most important in the context of cancer therapy, particularly copper nanoparticles, which are widely used. In this work, copper(II)-tyrosinase was isolated from potato peel powder. Copper nanoparticles (Tyr-Cu(II)-AEEA NPs) were synthesized via the reaction of tyrosinase with <i>N</i>-aminoethylethanolamine to produce Cu(II)-NPs and these were characterized by means of FT-IR, UV-Spectroscopy, XRD, SEM, TEM and a particle size analyzer. These Tyr-Cu(II)-AEEA NPs were tested as anticancer agents against MCF-7 breast cancer cells. Fluorescence microscopy and DNA fragmentation were also performed, which revealed the inhibiting potentials of Cu(II)-AEEA NPs and consequent cell death; Tyr-Cu(II)-AEEA NPs show potential cytotoxicity activity and this nano material could be contemplated as an anticancer medicament in future investigations.https://www.mdpi.com/1420-3049/26/21/6665copper nanoparticlesMCF-7 breast cancer cellcytotoxicity screeningDNA fragmentationfluorescence microscopy |
spellingShingle | Akbar Idhayadhulla Aseer Manilal Anis Ahamed Saud Alarifi Gurusamy Raman Potato Peels Mediated Synthesis of Cu(II)-nanoparticles from Tyrosinase Reacted with bis-(<i>N</i>-aminoethylethanolamine) (Tyr-Cu(II)-AEEA NPs) and Their Cytotoxicity against Michigan Cancer Foundation-7 Breast Cancer Cell Line Molecules copper nanoparticles MCF-7 breast cancer cell cytotoxicity screening DNA fragmentation fluorescence microscopy |
title | Potato Peels Mediated Synthesis of Cu(II)-nanoparticles from Tyrosinase Reacted with bis-(<i>N</i>-aminoethylethanolamine) (Tyr-Cu(II)-AEEA NPs) and Their Cytotoxicity against Michigan Cancer Foundation-7 Breast Cancer Cell Line |
title_full | Potato Peels Mediated Synthesis of Cu(II)-nanoparticles from Tyrosinase Reacted with bis-(<i>N</i>-aminoethylethanolamine) (Tyr-Cu(II)-AEEA NPs) and Their Cytotoxicity against Michigan Cancer Foundation-7 Breast Cancer Cell Line |
title_fullStr | Potato Peels Mediated Synthesis of Cu(II)-nanoparticles from Tyrosinase Reacted with bis-(<i>N</i>-aminoethylethanolamine) (Tyr-Cu(II)-AEEA NPs) and Their Cytotoxicity against Michigan Cancer Foundation-7 Breast Cancer Cell Line |
title_full_unstemmed | Potato Peels Mediated Synthesis of Cu(II)-nanoparticles from Tyrosinase Reacted with bis-(<i>N</i>-aminoethylethanolamine) (Tyr-Cu(II)-AEEA NPs) and Their Cytotoxicity against Michigan Cancer Foundation-7 Breast Cancer Cell Line |
title_short | Potato Peels Mediated Synthesis of Cu(II)-nanoparticles from Tyrosinase Reacted with bis-(<i>N</i>-aminoethylethanolamine) (Tyr-Cu(II)-AEEA NPs) and Their Cytotoxicity against Michigan Cancer Foundation-7 Breast Cancer Cell Line |
title_sort | potato peels mediated synthesis of cu ii nanoparticles from tyrosinase reacted with bis i n i aminoethylethanolamine tyr cu ii aeea nps and their cytotoxicity against michigan cancer foundation 7 breast cancer cell line |
topic | copper nanoparticles MCF-7 breast cancer cell cytotoxicity screening DNA fragmentation fluorescence microscopy |
url | https://www.mdpi.com/1420-3049/26/21/6665 |
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