Interaction between Two Iridovirus Core Proteins and Their Effects on Ranavirus (RGV) Replication in Cells from Different Species
The two putative proteins RGV-63R and RGV-91R encoded by <i>Rana grylio</i> virus (RGV) are DNA polymerase and proliferating cell nuclear antigen (PCNA) respectively, and are core proteins of iridoviruses. Here, the interaction between RGV-63R and RGV-91R was detected by a yeast two-hybr...
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MDPI AG
2019-05-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/11/5/416 |
| _version_ | 1818681509247188992 |
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| author | Xiao-Tao Zeng Qi-Ya Zhang |
| author_facet | Xiao-Tao Zeng Qi-Ya Zhang |
| author_sort | Xiao-Tao Zeng |
| collection | DOAJ |
| description | The two putative proteins RGV-63R and RGV-91R encoded by <i>Rana grylio</i> virus (RGV) are DNA polymerase and proliferating cell nuclear antigen (PCNA) respectively, and are core proteins of iridoviruses. Here, the interaction between RGV-63R and RGV-91R was detected by a yeast two-hybrid (Y2H) assay and further confirmed by co-immunoprecipitation (co-IP) assays. Subsequently, RGV-63R or RGV-91R were expressed alone or co-expressed in two kinds of aquatic animal cells including amphibian Chinese giant salamander thymus cells (GSTCs) and fish <i>Epithelioma papulosum cyprinid</i> cells (EPCs) to investigate their localizations and effects on RGV genome replication. The results showed that their localizations in the two kinds of cells are consistent. RGV-63R localized in the cytoplasm, while RGV-91R localized in the nucleus. However, when co-expressed, RGV-63R localized in both the cytoplasm and the nucleus, and colocalized with RGV-91R in the nucleus. 91R△NLS represents the RGV-91R deleting nuclear localization signal, which is localized in the cytoplasm and colocalized with RGV-63R in the cytoplasm. qPCR analysis revealed that sole expression and co-expression of the two proteins in the cells of two species significantly promoted RGV genome replication, while varying degrees of viral genome replication levels may be linked to the cell types. This study provides novel molecular evidence for ranavirus cross-species infection and replication. |
| first_indexed | 2024-12-17T10:04:05Z |
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| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-17T10:04:05Z |
| publishDate | 2019-05-01 |
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| series | Viruses |
| spelling | doaj.art-e13f694e18f4469aa532bc77afec06462022-12-21T21:53:13ZengMDPI AGViruses1999-49152019-05-0111541610.3390/v11050416v11050416Interaction between Two Iridovirus Core Proteins and Their Effects on Ranavirus (RGV) Replication in Cells from Different SpeciesXiao-Tao Zeng0Qi-Ya Zhang1State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Wuhan 430072, ChinaThe two putative proteins RGV-63R and RGV-91R encoded by <i>Rana grylio</i> virus (RGV) are DNA polymerase and proliferating cell nuclear antigen (PCNA) respectively, and are core proteins of iridoviruses. Here, the interaction between RGV-63R and RGV-91R was detected by a yeast two-hybrid (Y2H) assay and further confirmed by co-immunoprecipitation (co-IP) assays. Subsequently, RGV-63R or RGV-91R were expressed alone or co-expressed in two kinds of aquatic animal cells including amphibian Chinese giant salamander thymus cells (GSTCs) and fish <i>Epithelioma papulosum cyprinid</i> cells (EPCs) to investigate their localizations and effects on RGV genome replication. The results showed that their localizations in the two kinds of cells are consistent. RGV-63R localized in the cytoplasm, while RGV-91R localized in the nucleus. However, when co-expressed, RGV-63R localized in both the cytoplasm and the nucleus, and colocalized with RGV-91R in the nucleus. 91R△NLS represents the RGV-91R deleting nuclear localization signal, which is localized in the cytoplasm and colocalized with RGV-63R in the cytoplasm. qPCR analysis revealed that sole expression and co-expression of the two proteins in the cells of two species significantly promoted RGV genome replication, while varying degrees of viral genome replication levels may be linked to the cell types. This study provides novel molecular evidence for ranavirus cross-species infection and replication.https://www.mdpi.com/1999-4915/11/5/416<i>Rana grylio</i> virus (RGV)iridovirus core proteinsprotein interactionaquatic animalscross-species transmissionyeast two-hybrid (Y2H)co-immunoprecipitation (Co-IP) |
| spellingShingle | Xiao-Tao Zeng Qi-Ya Zhang Interaction between Two Iridovirus Core Proteins and Their Effects on Ranavirus (RGV) Replication in Cells from Different Species Viruses <i>Rana grylio</i> virus (RGV) iridovirus core proteins protein interaction aquatic animals cross-species transmission yeast two-hybrid (Y2H) co-immunoprecipitation (Co-IP) |
| title | Interaction between Two Iridovirus Core Proteins and Their Effects on Ranavirus (RGV) Replication in Cells from Different Species |
| title_full | Interaction between Two Iridovirus Core Proteins and Their Effects on Ranavirus (RGV) Replication in Cells from Different Species |
| title_fullStr | Interaction between Two Iridovirus Core Proteins and Their Effects on Ranavirus (RGV) Replication in Cells from Different Species |
| title_full_unstemmed | Interaction between Two Iridovirus Core Proteins and Their Effects on Ranavirus (RGV) Replication in Cells from Different Species |
| title_short | Interaction between Two Iridovirus Core Proteins and Their Effects on Ranavirus (RGV) Replication in Cells from Different Species |
| title_sort | interaction between two iridovirus core proteins and their effects on ranavirus rgv replication in cells from different species |
| topic | <i>Rana grylio</i> virus (RGV) iridovirus core proteins protein interaction aquatic animals cross-species transmission yeast two-hybrid (Y2H) co-immunoprecipitation (Co-IP) |
| url | https://www.mdpi.com/1999-4915/11/5/416 |
| work_keys_str_mv | AT xiaotaozeng interactionbetweentwoiridoviruscoreproteinsandtheireffectsonranavirusrgvreplicationincellsfromdifferentspecies AT qiyazhang interactionbetweentwoiridoviruscoreproteinsandtheireffectsonranavirusrgvreplicationincellsfromdifferentspecies |