Cytogenetic abnormalities in patients with hematological malignancies in Lahore city, Pakistan
Abstract Hematological and hematopoietic cells malignancies of the genes and hematopoietic cells are associated with the genetic mutation, often at the chromosomal level. The standard cytogenetic study is widely accepted as one of the main diagnostics and prognostic determinants in patients. Therefo...
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
2021-10-01
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| Series: | Brazilian Journal of Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100271&tlng=en |
| _version_ | 1798021392706306048 |
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| author | U. A. Awan N. Farooq A. Sarwar H. M. S. Jehangir M. S. Hashmi M. Alamgir F. Waheed M. Khurram H. Ahmed A. A. Khattak M. S. Afzal |
| author_facet | U. A. Awan N. Farooq A. Sarwar H. M. S. Jehangir M. S. Hashmi M. Alamgir F. Waheed M. Khurram H. Ahmed A. A. Khattak M. S. Afzal |
| author_sort | U. A. Awan |
| collection | DOAJ |
| description | Abstract Hematological and hematopoietic cells malignancies of the genes and hematopoietic cells are associated with the genetic mutation, often at the chromosomal level. The standard cytogenetic study is widely accepted as one of the main diagnostics and prognostic determinants in patients. Therefore, the current descriptive and cross-sectional study sought to determine the cytogenetic analysis of frequent hematological malignancies in Pakistan. A total of 202 peripheral bone marrow or blood samples from patients with benign and malignant hematological malignancy were taken using a conventional G-banding technique. Among enrolled patients, the mean age was 21.5 years ± 23.4, and gender-wise distribution showed a marked predominance of the male 147 (73%) population compared to the female 55 (27%). Patients in the age group (2-10 years) had the highest frequency, 48 (24%), of hematological neoplasms, followed by age (11-20 years) with 40 (20%). Normal karyotypes (46, XX/46, XY) was found in 51% (n=103) patients. Furthermore, the frequency of complex karyotype was 30 (15%), while normal was seen in 171 (85%) patients. Pre-B Acute Lymphoblastic Leukemia (Pre-B ALL) was the most prevalent malignancy of 66 (33%), followed by Chronic Myelogenous Leukemia (CML) of 41 (20%) and Acute Lymphocytic Leukemia of 29 (14%). Translocation was the most prevalent 50 (25%), followed by hypotriploidy 14 (7%) and monosomy 8 (4%) on chromosome aberration analysis. In addition, t(9:22) translocation was found to be 20 (10%) in CML, with the majority in the age group (31-40 years). This study recommends that karyotyping should be tested frequently in hematological conditions because it may provide insight into the relative chromosomal changes associated with particular malignancies. |
| first_indexed | 2024-04-11T17:13:08Z |
| format | Article |
| id | doaj.art-e144198c010b437f96aedfa31d2d9d25 |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-04-11T17:13:08Z |
| publishDate | 2021-10-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-e144198c010b437f96aedfa31d2d9d252022-12-22T04:12:51ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752021-10-018310.1590/1519-6984.249911Cytogenetic abnormalities in patients with hematological malignancies in Lahore city, PakistanU. A. Awanhttps://orcid.org/0000-0003-1183-2788N. FarooqA. SarwarH. M. S. JehangirM. S. HashmiM. AlamgirF. WaheedM. Khurramhttps://orcid.org/0000-0001-5804-5613H. Ahmedhttps://orcid.org/0000-0002-0382-3569A. A. Khattakhttps://orcid.org/0000-0002-9400-2984M. S. Afzalhttps://orcid.org/0000-0001-9807-5710Abstract Hematological and hematopoietic cells malignancies of the genes and hematopoietic cells are associated with the genetic mutation, often at the chromosomal level. The standard cytogenetic study is widely accepted as one of the main diagnostics and prognostic determinants in patients. Therefore, the current descriptive and cross-sectional study sought to determine the cytogenetic analysis of frequent hematological malignancies in Pakistan. A total of 202 peripheral bone marrow or blood samples from patients with benign and malignant hematological malignancy were taken using a conventional G-banding technique. Among enrolled patients, the mean age was 21.5 years ± 23.4, and gender-wise distribution showed a marked predominance of the male 147 (73%) population compared to the female 55 (27%). Patients in the age group (2-10 years) had the highest frequency, 48 (24%), of hematological neoplasms, followed by age (11-20 years) with 40 (20%). Normal karyotypes (46, XX/46, XY) was found in 51% (n=103) patients. Furthermore, the frequency of complex karyotype was 30 (15%), while normal was seen in 171 (85%) patients. Pre-B Acute Lymphoblastic Leukemia (Pre-B ALL) was the most prevalent malignancy of 66 (33%), followed by Chronic Myelogenous Leukemia (CML) of 41 (20%) and Acute Lymphocytic Leukemia of 29 (14%). Translocation was the most prevalent 50 (25%), followed by hypotriploidy 14 (7%) and monosomy 8 (4%) on chromosome aberration analysis. In addition, t(9:22) translocation was found to be 20 (10%) in CML, with the majority in the age group (31-40 years). This study recommends that karyotyping should be tested frequently in hematological conditions because it may provide insight into the relative chromosomal changes associated with particular malignancies.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100271&tlng=encytogenetic testinghematological malignancieschromosomesleukemia |
| spellingShingle | U. A. Awan N. Farooq A. Sarwar H. M. S. Jehangir M. S. Hashmi M. Alamgir F. Waheed M. Khurram H. Ahmed A. A. Khattak M. S. Afzal Cytogenetic abnormalities in patients with hematological malignancies in Lahore city, Pakistan Brazilian Journal of Biology cytogenetic testing hematological malignancies chromosomes leukemia |
| title | Cytogenetic abnormalities in patients with hematological malignancies in Lahore city, Pakistan |
| title_full | Cytogenetic abnormalities in patients with hematological malignancies in Lahore city, Pakistan |
| title_fullStr | Cytogenetic abnormalities in patients with hematological malignancies in Lahore city, Pakistan |
| title_full_unstemmed | Cytogenetic abnormalities in patients with hematological malignancies in Lahore city, Pakistan |
| title_short | Cytogenetic abnormalities in patients with hematological malignancies in Lahore city, Pakistan |
| title_sort | cytogenetic abnormalities in patients with hematological malignancies in lahore city pakistan |
| topic | cytogenetic testing hematological malignancies chromosomes leukemia |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100271&tlng=en |
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