Expression and Clinical Significance of TM9SF3 in Lung Adenocarcinoma
Objective To investigate the expression and clinical significance of TM9SF3 in lung adenocarcinoma (LUAD). Methods TCGA and GEPIA databases were used to screen the differentially-expressed TM9SF family molecules and analyze their effects on patient prognosis with LUAD. The expression and localizatio...
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| Format: | Article |
| Language: | zho |
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Magazine House of Cancer Research on Prevention and Treatment
2022-11-01
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| Series: | Zhongliu Fangzhi Yanjiu |
| Subjects: | |
| Online Access: | http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2022.22.0212 |
| _version_ | 1797819849536176128 |
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| author | YU Hualin XU Yinghua WANG Mingwei LI Shuguang ZHANG Wenwen YANG Jinsong LI Wei |
| author_facet | YU Hualin XU Yinghua WANG Mingwei LI Shuguang ZHANG Wenwen YANG Jinsong LI Wei |
| author_sort | YU Hualin |
| collection | DOAJ |
| description | Objective To investigate the expression and clinical significance of TM9SF3 in lung adenocarcinoma (LUAD). Methods TCGA and GEPIA databases were used to screen the differentially-expressed TM9SF family molecules and analyze their effects on patient prognosis with LUAD. The expression and localization of TM9SF3 in LUAD patients were verified by a human proteomic mapping database, Western blot assay, and polymerase chain reaction assay. Herein, the GSEA was used for the signal pathway enrichment analysis of TM9SF3-related genes. Meanwhile, the TIMER database and CIBERSORT algorithm were used to analyze the correlation between differentially-expressed TM9SF3 and the degree of immune cell infiltration. Results The expression of TM9SF3 in LUAD was significantly increased and had a significant adverse effect on the prognosis of LUAD patients. In addition, immunoblotting and polymerase chain reaction confirmed that TM9SF3 was highly expressed in LUAD. Meanwhile, the genes related to TM9SF3 expression were mainly enriched in cell signaling pathways regulating immune cell activity. The expression of TM9SF3 was significantly correlated with the expression changes of six immune cells. Conclusion TM9SF3 is differentially expressed in LUAD and may be used as a potential prognostic marker for LUAD patients. TM9SF3 can also change the level of immune cell infiltration in LUAD patients and is expected to be a new potential target for LUAD immunotherapy. |
| first_indexed | 2024-03-13T09:29:39Z |
| format | Article |
| id | doaj.art-e1457adeedaf464a93d404bc8af67d62 |
| institution | Directory Open Access Journal |
| issn | 1000-8578 |
| language | zho |
| last_indexed | 2024-03-13T09:29:39Z |
| publishDate | 2022-11-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj.art-e1457adeedaf464a93d404bc8af67d622023-05-26T04:23:49ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85782022-11-0149111146115210.3971/j.issn.1000-8578.2022.22.02128578.2022.22.0212Expression and Clinical Significance of TM9SF3 in Lung AdenocarcinomaYU Hualin0XU Yinghua1WANG Mingwei2LI Shuguang3ZHANG Wenwen4YANG Jinsong5LI Wei6Department of Radiotherapy, Affiliated Nantong Hospital 3 of Nantong University, Nantong 226001, ChinaDepartment of Radiotherapy, Affiliated Nantong Hospital 3 of Nantong University, Nantong 226001, ChinaDepartment of Radiotherapy, Affiliated Nantong Hospital 3 of Nantong University, Nantong 226001, ChinaDepartment of Radiotherapy, Affiliated Nantong Hospital 3 of Nantong University, Nantong 226001, ChinaDepartment of Radiotherapy, Affiliated Nantong Hospital 3 of Nantong University, Nantong 226001, ChinaDepartment of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, ChinaDepartment of Radiotherapy, Affiliated Nantong Hospital 3 of Nantong University, Nantong 226001, ChinaObjective To investigate the expression and clinical significance of TM9SF3 in lung adenocarcinoma (LUAD). Methods TCGA and GEPIA databases were used to screen the differentially-expressed TM9SF family molecules and analyze their effects on patient prognosis with LUAD. The expression and localization of TM9SF3 in LUAD patients were verified by a human proteomic mapping database, Western blot assay, and polymerase chain reaction assay. Herein, the GSEA was used for the signal pathway enrichment analysis of TM9SF3-related genes. Meanwhile, the TIMER database and CIBERSORT algorithm were used to analyze the correlation between differentially-expressed TM9SF3 and the degree of immune cell infiltration. Results The expression of TM9SF3 in LUAD was significantly increased and had a significant adverse effect on the prognosis of LUAD patients. In addition, immunoblotting and polymerase chain reaction confirmed that TM9SF3 was highly expressed in LUAD. Meanwhile, the genes related to TM9SF3 expression were mainly enriched in cell signaling pathways regulating immune cell activity. The expression of TM9SF3 was significantly correlated with the expression changes of six immune cells. Conclusion TM9SF3 is differentially expressed in LUAD and may be used as a potential prognostic marker for LUAD patients. TM9SF3 can also change the level of immune cell infiltration in LUAD patients and is expected to be a new potential target for LUAD immunotherapy.http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2022.22.0212lung adenocarcinomaimmunotherapytm9sf3prognosisimmune microenvironment |
| spellingShingle | YU Hualin XU Yinghua WANG Mingwei LI Shuguang ZHANG Wenwen YANG Jinsong LI Wei Expression and Clinical Significance of TM9SF3 in Lung Adenocarcinoma Zhongliu Fangzhi Yanjiu lung adenocarcinoma immunotherapy tm9sf3 prognosis immune microenvironment |
| title | Expression and Clinical Significance of TM9SF3 in Lung Adenocarcinoma |
| title_full | Expression and Clinical Significance of TM9SF3 in Lung Adenocarcinoma |
| title_fullStr | Expression and Clinical Significance of TM9SF3 in Lung Adenocarcinoma |
| title_full_unstemmed | Expression and Clinical Significance of TM9SF3 in Lung Adenocarcinoma |
| title_short | Expression and Clinical Significance of TM9SF3 in Lung Adenocarcinoma |
| title_sort | expression and clinical significance of tm9sf3 in lung adenocarcinoma |
| topic | lung adenocarcinoma immunotherapy tm9sf3 prognosis immune microenvironment |
| url | http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2022.22.0212 |
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