Design and Fabrication of Nanofibrous Dura Mater with Antifibrosis and Neuroprotection Effects on SH-SY5Y Cells
The development and treatment of some diseases, such as large-area cerebral infarction, cerebral hemorrhage, brain tumor, and craniocerebral trauma, which may involve the injury of the dura mater, elicit the need to repair this membrane by dural grafts. However, common dural grafts tend to result in...
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MDPI AG
2022-05-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/9/1882 |
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| author | Zhiyuan Zhao Tong Wu Yu Cui Rui Zhao Qi Wan Rui Xu |
| author_facet | Zhiyuan Zhao Tong Wu Yu Cui Rui Zhao Qi Wan Rui Xu |
| author_sort | Zhiyuan Zhao |
| collection | DOAJ |
| description | The development and treatment of some diseases, such as large-area cerebral infarction, cerebral hemorrhage, brain tumor, and craniocerebral trauma, which may involve the injury of the dura mater, elicit the need to repair this membrane by dural grafts. However, common dural grafts tend to result in dural adhesions and scar tissue and have no further neuroprotective effects. In order to reduce or avoid the complications of dural repair, we used PLGA, tetramethylpyrazine, and chitosan as raw materials to prepare a nanofibrous dura mater (NDM) with excellent biocompatibility and adequate mechanical characteristics, which can play a neuroprotective role and have an antifibrotic effect. We fabricated PLGA NDM by electrospinning, and then chitosan was grafted on the nanofibrous dura mater by the EDC-NHS cross-linking method to obtain PLGA/CS NDM. Then, we also prepared PLGA/TMP/CS NDM by coaxial electrospinning. Our study shows that the PLGA/TMP/CS NDM can inhibit the excessive proliferation of fibroblasts, as well as provide a sustained protective effect on the SH-SY5Y cells treated with oxygen–glucose deprivation/reperfusion (OGD/R). In conclusion, our study may provide a new alternative to dural grafts in undesirable cases of dural injuries. |
| first_indexed | 2024-03-10T03:46:19Z |
| format | Article |
| id | doaj.art-e145de36b49d4bf5a8c5e6e4673832a8 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T03:46:19Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-e145de36b49d4bf5a8c5e6e4673832a82023-11-23T09:07:39ZengMDPI AGPolymers2073-43602022-05-01149188210.3390/polym14091882Design and Fabrication of Nanofibrous Dura Mater with Antifibrosis and Neuroprotection Effects on SH-SY5Y CellsZhiyuan Zhao0Tong Wu1Yu Cui2Rui Zhao3Qi Wan4Rui Xu5Department of Interventional Radiology, The Affiliated Hospital of Qingdao University, Jiangsu Road 16, Qingdao 266000, ChinaInstitute of Neuroregeneration and Neurorehabilitation, Qingdao University, Qingdao 266071, ChinaInstitute of Neuroregeneration and Neurorehabilitation, Qingdao University, Qingdao 266071, ChinaDepartment of Interventional Radiology, The Affiliated Hospital of Qingdao University, Jiangsu Road 16, Qingdao 266000, ChinaInstitute of Neuroregeneration and Neurorehabilitation, Qingdao University, Qingdao 266071, ChinaDepartment of Interventional Radiology, The Affiliated Hospital of Qingdao University, Jiangsu Road 16, Qingdao 266000, ChinaThe development and treatment of some diseases, such as large-area cerebral infarction, cerebral hemorrhage, brain tumor, and craniocerebral trauma, which may involve the injury of the dura mater, elicit the need to repair this membrane by dural grafts. However, common dural grafts tend to result in dural adhesions and scar tissue and have no further neuroprotective effects. In order to reduce or avoid the complications of dural repair, we used PLGA, tetramethylpyrazine, and chitosan as raw materials to prepare a nanofibrous dura mater (NDM) with excellent biocompatibility and adequate mechanical characteristics, which can play a neuroprotective role and have an antifibrotic effect. We fabricated PLGA NDM by electrospinning, and then chitosan was grafted on the nanofibrous dura mater by the EDC-NHS cross-linking method to obtain PLGA/CS NDM. Then, we also prepared PLGA/TMP/CS NDM by coaxial electrospinning. Our study shows that the PLGA/TMP/CS NDM can inhibit the excessive proliferation of fibroblasts, as well as provide a sustained protective effect on the SH-SY5Y cells treated with oxygen–glucose deprivation/reperfusion (OGD/R). In conclusion, our study may provide a new alternative to dural grafts in undesirable cases of dural injuries.https://www.mdpi.com/2073-4360/14/9/1882nanofibrous dura materantifibrosisneuroprotectionPLGAtetramethylpyrazine |
| spellingShingle | Zhiyuan Zhao Tong Wu Yu Cui Rui Zhao Qi Wan Rui Xu Design and Fabrication of Nanofibrous Dura Mater with Antifibrosis and Neuroprotection Effects on SH-SY5Y Cells Polymers nanofibrous dura mater antifibrosis neuroprotection PLGA tetramethylpyrazine |
| title | Design and Fabrication of Nanofibrous Dura Mater with Antifibrosis and Neuroprotection Effects on SH-SY5Y Cells |
| title_full | Design and Fabrication of Nanofibrous Dura Mater with Antifibrosis and Neuroprotection Effects on SH-SY5Y Cells |
| title_fullStr | Design and Fabrication of Nanofibrous Dura Mater with Antifibrosis and Neuroprotection Effects on SH-SY5Y Cells |
| title_full_unstemmed | Design and Fabrication of Nanofibrous Dura Mater with Antifibrosis and Neuroprotection Effects on SH-SY5Y Cells |
| title_short | Design and Fabrication of Nanofibrous Dura Mater with Antifibrosis and Neuroprotection Effects on SH-SY5Y Cells |
| title_sort | design and fabrication of nanofibrous dura mater with antifibrosis and neuroprotection effects on sh sy5y cells |
| topic | nanofibrous dura mater antifibrosis neuroprotection PLGA tetramethylpyrazine |
| url | https://www.mdpi.com/2073-4360/14/9/1882 |
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