Evaluation of the gene encoding the enzyme βHPMEH for the bacterial wilt inhibition caused by Ralstonia solanacearum
Ralstonia solanacearum is the causal agent of the devastating bacterial wilt disease that attacks important agricultural crops such as potato, tomato, banana, among others, causing serious yield losses. Control of R. solanacearum is difficult because of its wide range of alternate hosts, its long su...
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| Format: | Article |
| Language: | English |
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Universidad Nacional Mayor de San Marcos
2015-10-01
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| Series: | Revista Peruana de Biología |
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| Online Access: | http://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/11353 |
| _version_ | 1811335152269262848 |
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| author | Elizabeth Fernandez Liliam Gutarra Jan Kreuze |
| author_facet | Elizabeth Fernandez Liliam Gutarra Jan Kreuze |
| author_sort | Elizabeth Fernandez |
| collection | DOAJ |
| description | Ralstonia solanacearum is the causal agent of the devastating bacterial wilt disease that attacks important agricultural crops such as potato, tomato, banana, among others, causing serious yield losses. Control of R. solanacearum is difficult because of its wide range of alternate hosts, its long survival in soil, its biological and genetic variation, the lack of natural resistance sources and the insufficiency of the appropriate chemical control measures. Quorum sensing is the term that describes the phenomenon whereby the accumulation of molecules allows bacteria to know the number of bacteria found in the environment (population density). R. solanacearum has a quorum sensing system for the regulation of the expression of virulence genes; the molecule 3-OH-PAME is the self-regulatory signal. The molecule ΒHPMEH hydrolyzes 3-OH-PAME nullifying the signal of virulence, and thus, the quorum sensing communication in R. solanacearum. In order to evaluate the βhpmeh gene we designed two vectors that express this gene under the control of two different promoters. Both vectors were verified by restriction analysis and sequencing. Agroinfiltration assays were used to analyze gene expression and the effect against R. solanacearum in potato (Solanum tuberosum) leaves. The results of the transient expression experiments showed that the expression of gene βhpmeh caused a delay in the appearance of symptoms of bacterial wilt and thus is a good candidate for whole genetic plant transformation. |
| first_indexed | 2024-04-13T17:19:55Z |
| format | Article |
| id | doaj.art-e14e8180a12041ba85106f4f4d1d79f8 |
| institution | Directory Open Access Journal |
| issn | 1561-0837 1727-9933 |
| language | English |
| last_indexed | 2024-04-13T17:19:55Z |
| publishDate | 2015-10-01 |
| publisher | Universidad Nacional Mayor de San Marcos |
| record_format | Article |
| series | Revista Peruana de Biología |
| spelling | doaj.art-e14e8180a12041ba85106f4f4d1d79f82022-12-22T02:38:02ZengUniversidad Nacional Mayor de San MarcosRevista Peruana de Biología1561-08371727-99332015-10-0122219319810.15381/rpb.v22i2.1135310072Evaluation of the gene encoding the enzyme βHPMEH for the bacterial wilt inhibition caused by Ralstonia solanacearumElizabeth Fernandez0Liliam Gutarra1Jan Kreuze2Laboratorio de Bacteriología y Biotecnología Aplicada, Centro Internacional de la Papa, Apartado 1558, Lima 12, Perú.Laboratorio de Bacteriología y Biotecnología Aplicada, Centro Internacional de la Papa, Apartado 1558, Lima 12, Perú.Laboratorio de Bacteriología y Biotecnología Aplicada, Centro Internacional de la Papa, Apartado 1558, Lima 12, Perú.Ralstonia solanacearum is the causal agent of the devastating bacterial wilt disease that attacks important agricultural crops such as potato, tomato, banana, among others, causing serious yield losses. Control of R. solanacearum is difficult because of its wide range of alternate hosts, its long survival in soil, its biological and genetic variation, the lack of natural resistance sources and the insufficiency of the appropriate chemical control measures. Quorum sensing is the term that describes the phenomenon whereby the accumulation of molecules allows bacteria to know the number of bacteria found in the environment (population density). R. solanacearum has a quorum sensing system for the regulation of the expression of virulence genes; the molecule 3-OH-PAME is the self-regulatory signal. The molecule ΒHPMEH hydrolyzes 3-OH-PAME nullifying the signal of virulence, and thus, the quorum sensing communication in R. solanacearum. In order to evaluate the βhpmeh gene we designed two vectors that express this gene under the control of two different promoters. Both vectors were verified by restriction analysis and sequencing. Agroinfiltration assays were used to analyze gene expression and the effect against R. solanacearum in potato (Solanum tuberosum) leaves. The results of the transient expression experiments showed that the expression of gene βhpmeh caused a delay in the appearance of symptoms of bacterial wilt and thus is a good candidate for whole genetic plant transformation.http://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/11353Expresión transitoriamarchitez bacterianaquorum sensingRalstonia solanacearumquorum quenching. |
| spellingShingle | Elizabeth Fernandez Liliam Gutarra Jan Kreuze Evaluation of the gene encoding the enzyme βHPMEH for the bacterial wilt inhibition caused by Ralstonia solanacearum Revista Peruana de Biología Expresión transitoria marchitez bacteriana quorum sensing Ralstonia solanacearum quorum quenching. |
| title | Evaluation of the gene encoding the enzyme βHPMEH for the bacterial wilt inhibition caused by Ralstonia solanacearum |
| title_full | Evaluation of the gene encoding the enzyme βHPMEH for the bacterial wilt inhibition caused by Ralstonia solanacearum |
| title_fullStr | Evaluation of the gene encoding the enzyme βHPMEH for the bacterial wilt inhibition caused by Ralstonia solanacearum |
| title_full_unstemmed | Evaluation of the gene encoding the enzyme βHPMEH for the bacterial wilt inhibition caused by Ralstonia solanacearum |
| title_short | Evaluation of the gene encoding the enzyme βHPMEH for the bacterial wilt inhibition caused by Ralstonia solanacearum |
| title_sort | evaluation of the gene encoding the enzyme βhpmeh for the bacterial wilt inhibition caused by ralstonia solanacearum |
| topic | Expresión transitoria marchitez bacteriana quorum sensing Ralstonia solanacearum quorum quenching. |
| url | http://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/11353 |
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