Fucoidan prevent murine autoimmune diabetes via suppression TLR4-signaling pathways, regulation DC/Treg induced immune tolerance and improving gut microecology
Abstract Background This study was to investigate the effect and its possible mechanism of fucoidan on the development of spontaneous autoimmune diabetes in non-obese diabetic (NOD) mice. Methods 7-week-old NOD mice were randomly divided into three groups: control group, low-dose (300 mg/kg) and hig...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-12-01
|
| Series: | Nutrition & Metabolism |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12986-019-0392-1 |
| _version_ | 1819077541956157440 |
|---|---|
| author | Meilan Xue Hui Liang Xinqiang Ji Ying Liu Yinlin Ge Lin Hou Ting Sun |
| author_facet | Meilan Xue Hui Liang Xinqiang Ji Ying Liu Yinlin Ge Lin Hou Ting Sun |
| author_sort | Meilan Xue |
| collection | DOAJ |
| description | Abstract Background This study was to investigate the effect and its possible mechanism of fucoidan on the development of spontaneous autoimmune diabetes in non-obese diabetic (NOD) mice. Methods 7-week-old NOD mice were randomly divided into three groups: control group, low-dose (300 mg/kg) and high-dose (600 mg/kg) fucoidan-treatment groups. After 5 weeks of treatment, 10 mice per group were randomly selected to be sacrificed after feces collection. The remaining 12 mice per group were fed until 26 weeks of age to assess the incidence of diabetes. Results Treatment with fucoidan increased serum insulin level, delayed the onset and decreased the development of diabetes in NOD mice. Fucoidan reduced the levels of strong Th1 proinflammatory cytokines, but induced Th2-bias ed. cytokine response. And dentridic cells (DCs) in fucoidan treatment group were characterized as low expression of MHC class II and CD86 molecules. TLR4 expressions and the downstream molecules in pancreas were down-regulated in fucoidan-treated groups. There were significant differences in the composition of gut flora between NOD control group and fucoidan group. Lactobacillus and Akkermansia were significantly enriched in fucoidan group. Conclusions Fucoidan could prevent the development of autoimmune diabetes in NOD mice via regulating DC/Treg induced immune tolerance, improving gut microecology, down-regulating TLR4 signaling pathway, and maintaining pancreatic internal environment. |
| first_indexed | 2024-12-21T18:58:51Z |
| format | Article |
| id | doaj.art-e14fff56ad0a42ec9ba4be3bc9b9cc75 |
| institution | Directory Open Access Journal |
| issn | 1743-7075 |
| language | English |
| last_indexed | 2024-12-21T18:58:51Z |
| publishDate | 2019-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Nutrition & Metabolism |
| spelling | doaj.art-e14fff56ad0a42ec9ba4be3bc9b9cc752022-12-21T18:53:33ZengBMCNutrition & Metabolism1743-70752019-12-0116111510.1186/s12986-019-0392-1Fucoidan prevent murine autoimmune diabetes via suppression TLR4-signaling pathways, regulation DC/Treg induced immune tolerance and improving gut microecologyMeilan Xue0Hui Liang1Xinqiang Ji2Ying Liu3Yinlin Ge4Lin Hou5Ting Sun6Department of Biochemistry and Molecular Biology, Basic Medical College, Qingdao University of MedicineThe Institute of Human Nutrition, Qingdao University of MedicineDepartment of Gynaecology, the Affiliated Hospital of Qingdao UniversityDepartment of Biochemistry and Molecular Biology, Basic Medical College, Qingdao University of MedicineDepartment of Biochemistry and Molecular Biology, Basic Medical College, Qingdao University of MedicineDepartment of Biochemistry and Molecular Biology, Basic Medical College, Qingdao University of MedicineDepartment of Biochemistry and Molecular Biology, Basic Medical College, Qingdao University of MedicineAbstract Background This study was to investigate the effect and its possible mechanism of fucoidan on the development of spontaneous autoimmune diabetes in non-obese diabetic (NOD) mice. Methods 7-week-old NOD mice were randomly divided into three groups: control group, low-dose (300 mg/kg) and high-dose (600 mg/kg) fucoidan-treatment groups. After 5 weeks of treatment, 10 mice per group were randomly selected to be sacrificed after feces collection. The remaining 12 mice per group were fed until 26 weeks of age to assess the incidence of diabetes. Results Treatment with fucoidan increased serum insulin level, delayed the onset and decreased the development of diabetes in NOD mice. Fucoidan reduced the levels of strong Th1 proinflammatory cytokines, but induced Th2-bias ed. cytokine response. And dentridic cells (DCs) in fucoidan treatment group were characterized as low expression of MHC class II and CD86 molecules. TLR4 expressions and the downstream molecules in pancreas were down-regulated in fucoidan-treated groups. There were significant differences in the composition of gut flora between NOD control group and fucoidan group. Lactobacillus and Akkermansia were significantly enriched in fucoidan group. Conclusions Fucoidan could prevent the development of autoimmune diabetes in NOD mice via regulating DC/Treg induced immune tolerance, improving gut microecology, down-regulating TLR4 signaling pathway, and maintaining pancreatic internal environment.https://doi.org/10.1186/s12986-019-0392-1Type 1 diabetesNon-obese diabetic miceFucoidanImmune toleranceGut microecology |
| spellingShingle | Meilan Xue Hui Liang Xinqiang Ji Ying Liu Yinlin Ge Lin Hou Ting Sun Fucoidan prevent murine autoimmune diabetes via suppression TLR4-signaling pathways, regulation DC/Treg induced immune tolerance and improving gut microecology Nutrition & Metabolism Type 1 diabetes Non-obese diabetic mice Fucoidan Immune tolerance Gut microecology |
| title | Fucoidan prevent murine autoimmune diabetes via suppression TLR4-signaling pathways, regulation DC/Treg induced immune tolerance and improving gut microecology |
| title_full | Fucoidan prevent murine autoimmune diabetes via suppression TLR4-signaling pathways, regulation DC/Treg induced immune tolerance and improving gut microecology |
| title_fullStr | Fucoidan prevent murine autoimmune diabetes via suppression TLR4-signaling pathways, regulation DC/Treg induced immune tolerance and improving gut microecology |
| title_full_unstemmed | Fucoidan prevent murine autoimmune diabetes via suppression TLR4-signaling pathways, regulation DC/Treg induced immune tolerance and improving gut microecology |
| title_short | Fucoidan prevent murine autoimmune diabetes via suppression TLR4-signaling pathways, regulation DC/Treg induced immune tolerance and improving gut microecology |
| title_sort | fucoidan prevent murine autoimmune diabetes via suppression tlr4 signaling pathways regulation dc treg induced immune tolerance and improving gut microecology |
| topic | Type 1 diabetes Non-obese diabetic mice Fucoidan Immune tolerance Gut microecology |
| url | https://doi.org/10.1186/s12986-019-0392-1 |
| work_keys_str_mv | AT meilanxue fucoidanpreventmurineautoimmunediabetesviasuppressiontlr4signalingpathwaysregulationdctreginducedimmunetoleranceandimprovinggutmicroecology AT huiliang fucoidanpreventmurineautoimmunediabetesviasuppressiontlr4signalingpathwaysregulationdctreginducedimmunetoleranceandimprovinggutmicroecology AT xinqiangji fucoidanpreventmurineautoimmunediabetesviasuppressiontlr4signalingpathwaysregulationdctreginducedimmunetoleranceandimprovinggutmicroecology AT yingliu fucoidanpreventmurineautoimmunediabetesviasuppressiontlr4signalingpathwaysregulationdctreginducedimmunetoleranceandimprovinggutmicroecology AT yinlinge fucoidanpreventmurineautoimmunediabetesviasuppressiontlr4signalingpathwaysregulationdctreginducedimmunetoleranceandimprovinggutmicroecology AT linhou fucoidanpreventmurineautoimmunediabetesviasuppressiontlr4signalingpathwaysregulationdctreginducedimmunetoleranceandimprovinggutmicroecology AT tingsun fucoidanpreventmurineautoimmunediabetesviasuppressiontlr4signalingpathwaysregulationdctreginducedimmunetoleranceandimprovinggutmicroecology |