Slow Cone Reflectance Changes during Bleaching Determined by Adaptive Optics Scanning Laser Ophthalmoscope in Living Human Eyes.
To investigate the changes in the reflectance of human cone photoreceptors by an adaptive optics scanning laser ophthalmoscope (AO-SLO) during photobleaching. A custom-built AO-SLO with an observation light of 840-nm was used to measure the cone densities and the reflectance changes during bleaching...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4488269?pdf=render |
| _version_ | 1818335383574806528 |
|---|---|
| author | Masakazu Hirota Suguru Miyagawa Hiroyuki Kanda Takao Endo Tibor Karl Lohmann Tomomitsu Miyoshi Takeshi Morimoto Takashi Fujikado |
| author_facet | Masakazu Hirota Suguru Miyagawa Hiroyuki Kanda Takao Endo Tibor Karl Lohmann Tomomitsu Miyoshi Takeshi Morimoto Takashi Fujikado |
| author_sort | Masakazu Hirota |
| collection | DOAJ |
| description | To investigate the changes in the reflectance of human cone photoreceptors by an adaptive optics scanning laser ophthalmoscope (AO-SLO) during photobleaching. A custom-built AO-SLO with an observation light of 840-nm was used to measure the cone densities and the reflectance changes during bleaching by 630 nm red light emitting diodes. Measurements were made at 1° and 3° temporal to the fovea within an area of 1° × 1° in 8 eyes of 8 normal subjects. After dark-adaptation, images of the cone mosaics were recorded continuously for 5-min before, 5-min during, and after 5-min of light stimulation with a sampling rate of 5-Hz. The first positive peak (P1) was observed at 72.2 ± 15.0-s and a second positive peak (P2) at 257.5 ± 34.5-s at 1°. The increase of the reflectance of P1 was significantly larger at 1° (34.4 ± 13.9%) than at 3° (26.0 ± 10.5%; P = 0.03, Wilcoxon's signed rank test). The average cone density at 1° (51123.13 ± 1401.23 cells/mm2) was significantly larger than that at 3° (30876.13 ± 1459.28 cells/mm2; P <0.001, Wilcoxon's signed rank test). The changes in the reflectance of the cones during bleaching by red light had two peaks. The two peaks may be caused by regeneration of cone photopigment during bleaching. |
| first_indexed | 2024-12-13T14:22:33Z |
| format | Article |
| id | doaj.art-e151afa6486446a0bbb1af5aea69c0da |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T14:22:33Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e151afa6486446a0bbb1af5aea69c0da2022-12-21T23:42:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01106e013148510.1371/journal.pone.0131485Slow Cone Reflectance Changes during Bleaching Determined by Adaptive Optics Scanning Laser Ophthalmoscope in Living Human Eyes.Masakazu HirotaSuguru MiyagawaHiroyuki KandaTakao EndoTibor Karl LohmannTomomitsu MiyoshiTakeshi MorimotoTakashi FujikadoTo investigate the changes in the reflectance of human cone photoreceptors by an adaptive optics scanning laser ophthalmoscope (AO-SLO) during photobleaching. A custom-built AO-SLO with an observation light of 840-nm was used to measure the cone densities and the reflectance changes during bleaching by 630 nm red light emitting diodes. Measurements were made at 1° and 3° temporal to the fovea within an area of 1° × 1° in 8 eyes of 8 normal subjects. After dark-adaptation, images of the cone mosaics were recorded continuously for 5-min before, 5-min during, and after 5-min of light stimulation with a sampling rate of 5-Hz. The first positive peak (P1) was observed at 72.2 ± 15.0-s and a second positive peak (P2) at 257.5 ± 34.5-s at 1°. The increase of the reflectance of P1 was significantly larger at 1° (34.4 ± 13.9%) than at 3° (26.0 ± 10.5%; P = 0.03, Wilcoxon's signed rank test). The average cone density at 1° (51123.13 ± 1401.23 cells/mm2) was significantly larger than that at 3° (30876.13 ± 1459.28 cells/mm2; P <0.001, Wilcoxon's signed rank test). The changes in the reflectance of the cones during bleaching by red light had two peaks. The two peaks may be caused by regeneration of cone photopigment during bleaching.http://europepmc.org/articles/PMC4488269?pdf=render |
| spellingShingle | Masakazu Hirota Suguru Miyagawa Hiroyuki Kanda Takao Endo Tibor Karl Lohmann Tomomitsu Miyoshi Takeshi Morimoto Takashi Fujikado Slow Cone Reflectance Changes during Bleaching Determined by Adaptive Optics Scanning Laser Ophthalmoscope in Living Human Eyes. PLoS ONE |
| title | Slow Cone Reflectance Changes during Bleaching Determined by Adaptive Optics Scanning Laser Ophthalmoscope in Living Human Eyes. |
| title_full | Slow Cone Reflectance Changes during Bleaching Determined by Adaptive Optics Scanning Laser Ophthalmoscope in Living Human Eyes. |
| title_fullStr | Slow Cone Reflectance Changes during Bleaching Determined by Adaptive Optics Scanning Laser Ophthalmoscope in Living Human Eyes. |
| title_full_unstemmed | Slow Cone Reflectance Changes during Bleaching Determined by Adaptive Optics Scanning Laser Ophthalmoscope in Living Human Eyes. |
| title_short | Slow Cone Reflectance Changes during Bleaching Determined by Adaptive Optics Scanning Laser Ophthalmoscope in Living Human Eyes. |
| title_sort | slow cone reflectance changes during bleaching determined by adaptive optics scanning laser ophthalmoscope in living human eyes |
| url | http://europepmc.org/articles/PMC4488269?pdf=render |
| work_keys_str_mv | AT masakazuhirota slowconereflectancechangesduringbleachingdeterminedbyadaptiveopticsscanninglaserophthalmoscopeinlivinghumaneyes AT sugurumiyagawa slowconereflectancechangesduringbleachingdeterminedbyadaptiveopticsscanninglaserophthalmoscopeinlivinghumaneyes AT hiroyukikanda slowconereflectancechangesduringbleachingdeterminedbyadaptiveopticsscanninglaserophthalmoscopeinlivinghumaneyes AT takaoendo slowconereflectancechangesduringbleachingdeterminedbyadaptiveopticsscanninglaserophthalmoscopeinlivinghumaneyes AT tiborkarllohmann slowconereflectancechangesduringbleachingdeterminedbyadaptiveopticsscanninglaserophthalmoscopeinlivinghumaneyes AT tomomitsumiyoshi slowconereflectancechangesduringbleachingdeterminedbyadaptiveopticsscanninglaserophthalmoscopeinlivinghumaneyes AT takeshimorimoto slowconereflectancechangesduringbleachingdeterminedbyadaptiveopticsscanninglaserophthalmoscopeinlivinghumaneyes AT takashifujikado slowconereflectancechangesduringbleachingdeterminedbyadaptiveopticsscanninglaserophthalmoscopeinlivinghumaneyes |