FISH Chromosome Mapping Allowing Karyotype Analysis in Medicago truncatula Lines Jemalong J5 and R-108-1
Two lines of Medicago truncatula, R-108-1 and Jemalong J5, exhibit differences in their symbiotic properties, morphological markers, and capacity for regeneration and transformation. Using cytogenetic techniques, we detected differences in chromomycin A3 staining on the two sides of the secondary co...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
1999-11-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.11.947 |
| _version_ | 1811291357448241152 |
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| author | Malika Cerbah Zoltan Kevei Sonja Siljak-Yakovlev Eva Kondorosi Adam Kondorosi Toan Hanh Trinh |
| author_facet | Malika Cerbah Zoltan Kevei Sonja Siljak-Yakovlev Eva Kondorosi Adam Kondorosi Toan Hanh Trinh |
| author_sort | Malika Cerbah |
| collection | DOAJ |
| description | Two lines of Medicago truncatula, R-108-1 and Jemalong J5, exhibit differences in their symbiotic properties, morphological markers, and capacity for regeneration and transformation. Using cytogenetic techniques, we detected differences in chromomycin A3 staining on the two sides of the secondary constriction of the nucleolar chromosome pair of J5 and R-108-1, while DAPI (4′, 6-diamidino-2-phenylindole) staining revealed no differences between the two lines. FISH (fluorescent in situ hybridization) analysis showed a difference between J5 and R-108-1 for the same 5S rRNA genes; three loci were found in J5 but only two loci in R-108-1. However, for the 18S-5.8S-26S rDNA genes only a single locus was found in both lines. Based on these analyses, a karyotype for the two lines was established. |
| first_indexed | 2024-04-13T04:28:07Z |
| format | Article |
| id | doaj.art-e1542d0082e0473aa8ef7cd026f16ec5 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:28:07Z |
| publishDate | 1999-11-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-e1542d0082e0473aa8ef7cd026f16ec52022-12-22T03:02:27ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77061999-11-01121194795010.1094/MPMI.1999.12.11.947FISH Chromosome Mapping Allowing Karyotype Analysis in Medicago truncatula Lines Jemalong J5 and R-108-1Malika CerbahZoltan KeveiSonja Siljak-YakovlevEva KondorosiAdam KondorosiToan Hanh TrinhTwo lines of Medicago truncatula, R-108-1 and Jemalong J5, exhibit differences in their symbiotic properties, morphological markers, and capacity for regeneration and transformation. Using cytogenetic techniques, we detected differences in chromomycin A3 staining on the two sides of the secondary constriction of the nucleolar chromosome pair of J5 and R-108-1, while DAPI (4′, 6-diamidino-2-phenylindole) staining revealed no differences between the two lines. FISH (fluorescent in situ hybridization) analysis showed a difference between J5 and R-108-1 for the same 5S rRNA genes; three loci were found in J5 but only two loci in R-108-1. However, for the 18S-5.8S-26S rDNA genes only a single locus was found in both lines. Based on these analyses, a karyotype for the two lines was established.https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.11.947 |
| spellingShingle | Malika Cerbah Zoltan Kevei Sonja Siljak-Yakovlev Eva Kondorosi Adam Kondorosi Toan Hanh Trinh FISH Chromosome Mapping Allowing Karyotype Analysis in Medicago truncatula Lines Jemalong J5 and R-108-1 Molecular Plant-Microbe Interactions |
| title | FISH Chromosome Mapping Allowing Karyotype Analysis in Medicago truncatula Lines Jemalong J5 and R-108-1 |
| title_full | FISH Chromosome Mapping Allowing Karyotype Analysis in Medicago truncatula Lines Jemalong J5 and R-108-1 |
| title_fullStr | FISH Chromosome Mapping Allowing Karyotype Analysis in Medicago truncatula Lines Jemalong J5 and R-108-1 |
| title_full_unstemmed | FISH Chromosome Mapping Allowing Karyotype Analysis in Medicago truncatula Lines Jemalong J5 and R-108-1 |
| title_short | FISH Chromosome Mapping Allowing Karyotype Analysis in Medicago truncatula Lines Jemalong J5 and R-108-1 |
| title_sort | fish chromosome mapping allowing karyotype analysis in medicago truncatula lines jemalong j5 and r 108 1 |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.11.947 |
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