Redox-Active Pyocyanin Secreted by Pseudomonas aeruginosa 7NSK2 Triggers Systemic Resistance to Magnaporthe grisea but Enhances Rhizoctonia solani Susceptibility in Rice
Pseudomonas aeruginosa 7NSK2 induces resistance in dicots through a synergistic interaction of the phenazine pyocyanin and the salicylic acid-derivative pyochelin. Root inoculation of the monocot model rice with 7NSK2 partially protected leaves against blast disease (Magnaporthe grisea) but failed t...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2006-12-01
|
| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-19-1406 |
| _version_ | 1811290238553686016 |
|---|---|
| author | David De Vleesschauwer Pierre Cornelis Monica Höfte |
| author_facet | David De Vleesschauwer Pierre Cornelis Monica Höfte |
| author_sort | David De Vleesschauwer |
| collection | DOAJ |
| description | Pseudomonas aeruginosa 7NSK2 induces resistance in dicots through a synergistic interaction of the phenazine pyocyanin and the salicylic acid-derivative pyochelin. Root inoculation of the monocot model rice with 7NSK2 partially protected leaves against blast disease (Magnaporthe grisea) but failed to consistently reduce sheath blight (Rhizoctonia solani). Only mutations interfering with pyocyanin production led to a significant decrease in induced systemic resistance (ISR) to M. grisea, and in trans complementation for pyocyanin production restored the ability to elicit ISR. Intriguingly, pyocyanin-deficient mutants, unlike the wild type, triggered ISR against R. solani. Hence, bacterial pyocyanin plays a differential role in 7NSK2-mediated ISR in rice. Application of purified pyocyanin to hydroponically grown rice seedlings increased H2O2 levels locally on the root surface as well as a biphasic H2O2 generation pattern in distal leaves. Co-application of pyocyanin and the antioxidant sodium ascorbate alleviated the opposite effects of pyocyanin on rice blast and sheath blight development, suggesting that the differential effectiveness of pyocyanin with respect to 7NSK2-triggered ISR is mediated by transiently elevated H2O2 levels in planta. The cumulative results suggest that reactive oxygen species act as a double-edged sword in the interaction of rice with the hemibiotroph M. grisea and the necrotroph R. solani. |
| first_indexed | 2024-04-13T04:09:23Z |
| format | Article |
| id | doaj.art-e154d829a4b44be3ace0053ea83ce1d7 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:09:23Z |
| publishDate | 2006-12-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-e154d829a4b44be3ace0053ea83ce1d72022-12-22T03:03:10ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062006-12-0119121406141910.1094/MPMI-19-1406Redox-Active Pyocyanin Secreted by Pseudomonas aeruginosa 7NSK2 Triggers Systemic Resistance to Magnaporthe grisea but Enhances Rhizoctonia solani Susceptibility in RiceDavid De VleesschauwerPierre CornelisMonica HöftePseudomonas aeruginosa 7NSK2 induces resistance in dicots through a synergistic interaction of the phenazine pyocyanin and the salicylic acid-derivative pyochelin. Root inoculation of the monocot model rice with 7NSK2 partially protected leaves against blast disease (Magnaporthe grisea) but failed to consistently reduce sheath blight (Rhizoctonia solani). Only mutations interfering with pyocyanin production led to a significant decrease in induced systemic resistance (ISR) to M. grisea, and in trans complementation for pyocyanin production restored the ability to elicit ISR. Intriguingly, pyocyanin-deficient mutants, unlike the wild type, triggered ISR against R. solani. Hence, bacterial pyocyanin plays a differential role in 7NSK2-mediated ISR in rice. Application of purified pyocyanin to hydroponically grown rice seedlings increased H2O2 levels locally on the root surface as well as a biphasic H2O2 generation pattern in distal leaves. Co-application of pyocyanin and the antioxidant sodium ascorbate alleviated the opposite effects of pyocyanin on rice blast and sheath blight development, suggesting that the differential effectiveness of pyocyanin with respect to 7NSK2-triggered ISR is mediated by transiently elevated H2O2 levels in planta. The cumulative results suggest that reactive oxygen species act as a double-edged sword in the interaction of rice with the hemibiotroph M. grisea and the necrotroph R. solani.https://apsjournals.apsnet.org/doi/10.1094/MPMI-19-1406hypersensitive responseoxidative burstPyricularia grisearhizobacteria |
| spellingShingle | David De Vleesschauwer Pierre Cornelis Monica Höfte Redox-Active Pyocyanin Secreted by Pseudomonas aeruginosa 7NSK2 Triggers Systemic Resistance to Magnaporthe grisea but Enhances Rhizoctonia solani Susceptibility in Rice Molecular Plant-Microbe Interactions hypersensitive response oxidative burst Pyricularia grisea rhizobacteria |
| title | Redox-Active Pyocyanin Secreted by Pseudomonas aeruginosa 7NSK2 Triggers Systemic Resistance to Magnaporthe grisea but Enhances Rhizoctonia solani Susceptibility in Rice |
| title_full | Redox-Active Pyocyanin Secreted by Pseudomonas aeruginosa 7NSK2 Triggers Systemic Resistance to Magnaporthe grisea but Enhances Rhizoctonia solani Susceptibility in Rice |
| title_fullStr | Redox-Active Pyocyanin Secreted by Pseudomonas aeruginosa 7NSK2 Triggers Systemic Resistance to Magnaporthe grisea but Enhances Rhizoctonia solani Susceptibility in Rice |
| title_full_unstemmed | Redox-Active Pyocyanin Secreted by Pseudomonas aeruginosa 7NSK2 Triggers Systemic Resistance to Magnaporthe grisea but Enhances Rhizoctonia solani Susceptibility in Rice |
| title_short | Redox-Active Pyocyanin Secreted by Pseudomonas aeruginosa 7NSK2 Triggers Systemic Resistance to Magnaporthe grisea but Enhances Rhizoctonia solani Susceptibility in Rice |
| title_sort | redox active pyocyanin secreted by pseudomonas aeruginosa 7nsk2 triggers systemic resistance to magnaporthe grisea but enhances rhizoctonia solani susceptibility in rice |
| topic | hypersensitive response oxidative burst Pyricularia grisea rhizobacteria |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-19-1406 |
| work_keys_str_mv | AT daviddevleesschauwer redoxactivepyocyaninsecretedbypseudomonasaeruginosa7nsk2triggerssystemicresistancetomagnaporthegriseabutenhancesrhizoctoniasolanisusceptibilityinrice AT pierrecornelis redoxactivepyocyaninsecretedbypseudomonasaeruginosa7nsk2triggerssystemicresistancetomagnaporthegriseabutenhancesrhizoctoniasolanisusceptibilityinrice AT monicahofte redoxactivepyocyaninsecretedbypseudomonasaeruginosa7nsk2triggerssystemicresistancetomagnaporthegriseabutenhancesrhizoctoniasolanisusceptibilityinrice |